Whole‐cell biosynthesis of cytarabine by an unnecessary protein‐reduced Escherichia coli that coexpresses purine and uracil phosphorylase

• Published in: Biotechnology and bioengineering Vol. 119; no. 7; pp. 1768 - 1780
• Main Authors: Ping, Li, Ruxian, Jing, Mengping, Zhou, Pei, Jia, Zhuoya, Li, Guosheng, Liu, Zhenyu, Wang, Hailei, Wang
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01.07.2022
• Subjects: Adenine; Biosynthesis; Catalysis; Catalysts; Chemical synthesis; Conversion; Cytarabine; E coli; Enzymes; Escherichia coli; gene deletion; Genetic modification; Genomes; Interference; Phosphorylase; Phosphorylases; Protein purification; Proteins; Substrates; unnecessary proteins; Uracil; whole‐cell catalysis; cytarabine; unnecessary proteins; whole-cell catalysis; Escherichia coli; gene deletion

Currently, whole‐cell catalysts face challenges due to the complexity of reaction systems, although they have a cost advantage over pure enzymes. In this study, cytarabine was synthesized by purified purine phosphorylase 1 (PNP1) and uracil phosphorylase (UP), and the conversion of cytarabine from a...