Characterization of a Cu2+, SDS, alcohol and glucose tolerant GH1 β-glucosidase from Bacillus sp. CGMCC 1.16541

• Published in: Antonie van Leeuwenhoek Vol. 113; no. 10; pp. 1467 - 1477
• Main Authors: Yin, Yi-Rui, Sang, Peng, Yang, Feng-Li, Li, Tao, Yang, Run-Feng, Liu, Hong-Yan, Luo, Zong-Long, Li, Wen-Jun, Yang, Li-Quan
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.10.2020; Springer Nature B.V
• Subjects: Alcohol; Alcohols; Amino acid sequence; Amino acids; Bacillus; Bagasse; Biomedical and Life Sciences; Cellobiase; Cellobiose; Cellulase; Cellulose; Copper; Cupric ions; Drug tolerance; E coli; Glucose; Glucose tolerance; Glucosidase; Industrial applications; Life Sciences; Medical Microbiology; Microbiology; Molecular weight; Original Paper; p-Nitrophenyl-b-D-glucopyranoside; pH effects; Plant Sciences; Sodium dodecyl sulfate; Sodium lauryl sulfate; Soil Science & Conservation; Sugarcane; Xylopyranoside; β-Glucosidase; Glucose tolerance; β-Glucosidase; Cu; SDS; Alcohol; sp; Heterogeneous expression

A β-glucosidase gene ( bsbgl1a ) from Bacillus sp. CGMCC 1.16541 was expressed in Escherichia coli BL21 and subsequently characterized. The amino acid sequence shared 83.64% identity with β-glucosidase (WP_066390903.1) from Fictibacillus phosphorivorans . The recombinant β-glucosidase (BsBgl1A) had...