Stain-free enucleation of mouse and human oocytes with a 1033 nm femtosecond laser

• Published in: Journal of biomedical optics Vol. 29; no. 6; p. 065002
• Main Authors: Osychenko, Alina A, Zalessky, Alexandr D, Bachurin, Alexey V, Martirosyan, David Yu, Egorova, Maria S, Nadtochenko, Viktor A
• Format: Journal Article
• Language: English
• Published: United States Society of Photo-Optical Instrumentation Engineers 01.06.2024
• Subjects: Animals; Female; Humans; Lasers; Metaphase; Mice; Microscopy, Confocal - methods; Microscopy, Polarization - methods; Oocytes - cytology; Spindle Apparatus; recipient cytoplast; femtosecond laser; spindle; enucleation

Preparation of a recipient cytoplast by oocyte enucleation is an essential task for animal cloning and assisted reproductive technologies in humans. The femtosecond laser is a precise and low-invasive tool for oocyte enucleation, and it should be an appropriate alternative to traditional enucleation...