Examination of pretreatment methods for DNA extraction from nails
In the conventional method of DNA extraction from nails, it takes approximately half a day to dissolve the nails. In this study, we examined whether using the HOrizontal Nail MAshing (HONMA) method, in which pressure is applied to the nail to crush it flat and increase its surface area, would improv...
Saved in:
Published in | Forensic science international. Genetics supplement series Vol. 8; pp. 110 - 111 |
---|---|
Main Authors | , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Elsevier B.V
01.12.2022
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | In the conventional method of DNA extraction from nails, it takes approximately half a day to dissolve the nails. In this study, we examined whether using the HOrizontal Nail MAshing (HONMA) method, in which pressure is applied to the nail to crush it flat and increase its surface area, would improve DNA extraction efficiency. Fingernails (5 mg) provided by ten volunteers were used as samples. Nail pieces (1–3 pieces), shredded with nail clippers, were thinly stretched by applying 2 t of pressure to each piece using a hydraulic press. DNA was extracted by incubation at 56 °C for 10 min and 1 h during proteolysis. DNA yield from the nails pretreated using the HONMA method increased by 0.20–7.10 times compared with that from unprocessed nails. In particular, 10-min incubation using the HONMA method resulted in an average 2.05-fold increase in DNA yield compared with that under overnight incubation. However, the impact of using the HONMA method varied widely among individuals, and the amount of extracted DNA decreased in some cases, suggesting that the yield may differ depending on the nail quality. |
---|---|
AbstractList | In the conventional method of DNA extraction from nails, it takes approximately half a day to dissolve the nails. In this study, we examined whether using the HOrizontal Nail MAshing (HONMA) method, in which pressure is applied to the nail to crush it flat and increase its surface area, would improve DNA extraction efficiency. Fingernails (5 mg) provided by ten volunteers were used as samples. Nail pieces (1–3 pieces), shredded with nail clippers, were thinly stretched by applying 2 t of pressure to each piece using a hydraulic press. DNA was extracted by incubation at 56 °C for 10 min and 1 h during proteolysis. DNA yield from the nails pretreated using the HONMA method increased by 0.20–7.10 times compared with that from unprocessed nails. In particular, 10-min incubation using the HONMA method resulted in an average 2.05-fold increase in DNA yield compared with that under overnight incubation. However, the impact of using the HONMA method varied widely among individuals, and the amount of extracted DNA decreased in some cases, suggesting that the yield may differ depending on the nail quality. |
Author | Asogawa, Minoru Nakamaru, Naomi Satoh, Fumiko Sugisawa, Masatoshi Sasaki, Chizuko Saito, Junichiro Sakamoto, Momoko Ochiai, Eriko Nagato, Junpei Irie, Wataru |
Author_xml | – sequence: 1 givenname: Eriko surname: Ochiai fullname: Ochiai, Eriko email: ochiai@med.kitasato-u.ac.jp organization: Department of Legal Medicine, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami-ku, Sagamihara, Kanagawa 252-0374, Japan – sequence: 2 givenname: Minoru surname: Asogawa fullname: Asogawa, Minoru organization: Department of Legal Medicine, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami-ku, Sagamihara, Kanagawa 252-0374, Japan – sequence: 3 givenname: Wataru surname: Irie fullname: Irie, Wataru organization: Department of Legal Medicine, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami-ku, Sagamihara, Kanagawa 252-0374, Japan – sequence: 4 givenname: Chizuko surname: Sasaki fullname: Sasaki, Chizuko organization: Department of Legal Medicine, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami-ku, Sagamihara, Kanagawa 252-0374, Japan – sequence: 5 givenname: Naomi surname: Nakamaru fullname: Nakamaru, Naomi organization: Department of Legal Medicine, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami-ku, Sagamihara, Kanagawa 252-0374, Japan – sequence: 6 givenname: Momoko surname: Sakamoto fullname: Sakamoto, Momoko organization: Department of Legal Medicine, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami-ku, Sagamihara, Kanagawa 252-0374, Japan – sequence: 7 givenname: Junpei surname: Nagato fullname: Nagato, Junpei organization: Department of Legal Medicine, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami-ku, Sagamihara, Kanagawa 252-0374, Japan – sequence: 8 givenname: Junichiro surname: Saito fullname: Saito, Junichiro organization: Department of Legal Medicine, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami-ku, Sagamihara, Kanagawa 252-0374, Japan – sequence: 9 givenname: Masatoshi surname: Sugisawa fullname: Sugisawa, Masatoshi organization: 1st Public Security Solutions Department, NEC Corporation, 5-7-1 Shiba, Minato-ku, Tokyo 108-8001, Japan – sequence: 10 givenname: Fumiko surname: Satoh fullname: Satoh, Fumiko organization: Department of Legal Medicine, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami-ku, Sagamihara, Kanagawa 252-0374, Japan |
BookMark | eNp9kE1LAzEYhIMo2Fb_gYf8gV3fZL-Si1Bq_YCilx68hWz2jaZ0k5IEqf_e1opHTzOHmWF4puTcB4-E3DAoGbD2dlPa5N5TKjlwXoIsoZJnZMJE1xSsa97O_3wrLsk0pQ1AByDrCZkv93p0XmcXPA2W7iLmiDqP6DMdMX-EIVEbIr1_mVPc56jNT9TGMFKv3TZdkQurtwmvf3VG1g_L9eKpWL0-Pi_mq8JwwXPB-wGlqLGBrmmYMBIYMt0zgVzUrZa6NgOrLaAxaFlvq77qDbSyRYmmqaoZqU-zJoaUIlq1i27U8UsxUEcKaqNOFNSRggKpDhQOtbtTDQ_XPh1GlYxDb3BwEU1WQ3D_D3wDsK5rAw |
CitedBy_id | crossref_primary_10_1080_00032719_2023_2296671 |
Cites_doi | 10.1016/j.fsigen.2008.07.008 10.1007/s00414-020-02289-x 10.1016/j.jmoldx.2014.02.004 |
ContentType | Journal Article |
Copyright | 2022 Elsevier B.V. |
Copyright_xml | – notice: 2022 Elsevier B.V. |
DBID | AAYXX CITATION |
DOI | 10.1016/j.fsigss.2022.09.039 |
DatabaseName | CrossRef |
DatabaseTitle | CrossRef |
DatabaseTitleList | |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Public Health |
EISSN | 1875-175X |
EndPage | 111 |
ExternalDocumentID | 10_1016_j_fsigss_2022_09_039 S1875176822000373 |
GroupedDBID | --- --K --M .~1 0R~ 1B1 1~. 1~5 4.4 457 4G. 53G 5GY 5VS 7-5 71M 8P~ AACTN AAEDT AAEDW AAFJI AAIAV AAIKJ AAKOC AALRI AAOAW AAQFI AAXUO ABBQC ABGSF ABLVK ABMAC ABMMH ABMZM ABUDA ABXDB ABYKQ ACDAQ ACGFS ACRLP ACXMD ADBBV ADEZE ADMUD ADUVX AEBSH AEHWI AEKER AFKWA AFTJW AFXIZ AGHFR AGUBO AGYEJ AIEXJ AIKHN AITUG AJBFU AJOXV AJRQY AKYCK ALMA_UNASSIGNED_HOLDINGS AMFUW AMRAJ ANZVX AOMHK AVARZ AXJTR BKOJK BLXMC BNPGV CS3 DOVZS EBS EFJIC EFLBG EJD EP2 EP3 FDB FEDTE FIRID FNPLU FYGXN GBLVA HVGLF HX~ HZ~ J1W KOM LCYCR M41 MO0 N9A O-L O9- OAUVE OZT P-8 P-9 PC. PRBVW Q38 RIG ROL SDF SES SPCBC SSB SSH SSO SSU SSZ T5K ~G- AAXKI AAYXX ADVLN AFJKZ AKRWK CITATION |
ID | FETCH-LOGICAL-c282t-2bde984e5075518c901e1ab18e2846a9a4cd14f0eccef1bf3b3bc0696e9ec533 |
IEDL.DBID | AIKHN |
ISSN | 1875-1768 |
IngestDate | Thu Sep 26 17:42:08 EDT 2024 Fri Feb 23 02:39:34 EST 2024 |
IsDoiOpenAccess | false |
IsOpenAccess | true |
IsPeerReviewed | true |
IsScholarly | true |
Keywords | Personal identification DNA extraction Nail |
Language | English |
LinkModel | DirectLink |
MergedId | FETCHMERGED-LOGICAL-c282t-2bde984e5075518c901e1ab18e2846a9a4cd14f0eccef1bf3b3bc0696e9ec533 |
OpenAccessLink | http://manuscript.elsevier.com/S1875176822000373/pdf/S1875176822000373.pdf |
PageCount | 2 |
ParticipantIDs | crossref_primary_10_1016_j_fsigss_2022_09_039 elsevier_sciencedirect_doi_10_1016_j_fsigss_2022_09_039 |
PublicationCentury | 2000 |
PublicationDate | December 2022 2022-12-00 |
PublicationDateYYYYMMDD | 2022-12-01 |
PublicationDate_xml | – month: 12 year: 2022 text: December 2022 |
PublicationDecade | 2020 |
PublicationTitle | Forensic science international. Genetics supplement series |
PublicationYear | 2022 |
Publisher | Elsevier B.V |
Publisher_xml | – name: Elsevier B.V |
References | Preuner, Danzer, Pröll (bib2) 2014; 16 Allouche, Hamdoum, Mangin (bib1) 2008; 3 Inkret, Podovšovnik, Zupanc (bib3) 2020; 134 Preuner (10.1016/j.fsigss.2022.09.039_bib2) 2014; 16 Inkret (10.1016/j.fsigss.2022.09.039_bib3) 2020; 134 Allouche (10.1016/j.fsigss.2022.09.039_bib1) 2008; 3 |
References_xml | – volume: 3 start-page: 46 year: 2008 end-page: 49 ident: bib1 article-title: Genetic identification of decomposed cadavers using nails as DNA source publication-title: Forensic Sci. Int. Genet. contributor: fullname: Mangin – volume: 16 start-page: 459 year: 2014 end-page: 466 ident: bib2 article-title: High-Quality DNA from fingernails for genetic analysis publication-title: J. Mol. Diagn. contributor: fullname: Pröll – volume: 134 start-page: 1629 year: 2020 end-page: 1638 ident: bib3 article-title: Nails as a primary sample type for molecular genetic identification of highly decomposed human remains publication-title: Int. J. Leg. Med. contributor: fullname: Zupanc – volume: 3 start-page: 46 issue: 1 year: 2008 ident: 10.1016/j.fsigss.2022.09.039_bib1 article-title: Genetic identification of decomposed cadavers using nails as DNA source publication-title: Forensic Sci. Int. Genet. doi: 10.1016/j.fsigen.2008.07.008 contributor: fullname: Allouche – volume: 134 start-page: 1629 year: 2020 ident: 10.1016/j.fsigss.2022.09.039_bib3 article-title: Nails as a primary sample type for molecular genetic identification of highly decomposed human remains publication-title: Int. J. Leg. Med. doi: 10.1007/s00414-020-02289-x contributor: fullname: Inkret – volume: 16 start-page: 459 issue: 4 year: 2014 ident: 10.1016/j.fsigss.2022.09.039_bib2 article-title: High-Quality DNA from fingernails for genetic analysis publication-title: J. Mol. Diagn. doi: 10.1016/j.jmoldx.2014.02.004 contributor: fullname: Preuner |
SSID | ssj0070094 |
Score | 2.300866 |
Snippet | In the conventional method of DNA extraction from nails, it takes approximately half a day to dissolve the nails. In this study, we examined whether using the... |
SourceID | crossref elsevier |
SourceType | Aggregation Database Publisher |
StartPage | 110 |
SubjectTerms | DNA extraction Nail Personal identification |
Title | Examination of pretreatment methods for DNA extraction from nails |
URI | https://dx.doi.org/10.1016/j.fsigss.2022.09.039 |
Volume | 8 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3PS8MwGP2Y20UQ8SfOHyMHr3VNk3XNscyN6WAHnbhbaNpEKtKNdYIn_3a_NC0oiAdPpaUp5aV830t57wXgmvNBKgaDxAtSxj3OlO9FGVWeSUSYRcIuQqq0z3k4feL3y8GyBaPGC2NllXXtdzW9qtb1lX6NZn-d5_1HilSbIlsOgipFhe1AB9sR523oxHez6bwpyEOrnrPrLrzf5iFGjYOuknmZMn8pbW53ELjAU_F7h_rWdSYHsF_TRRK7NzqEli6OYM_9ayPOQnQM8fgjsYoWizFZGWI1hI1-nLgtokuC5JTczmOCxXjjzAzEWktIkeRv5QksJuPFaOrVWyN4Ka6Rtl6gMi0irpHN2Ui1FLu6pomikcZ2EyYi4WlGufFxgrShyjDFVOqHItRCp8jwTqFdrAp9BmQYqoz5lqVEhjPDhLVQ4TMi7OUBZboLXoOGXLsADNkow16lQ09a9KQvJKLXhWEDmfwxkRJr9J8jz_898gJ27ZlTmVxCe7t511fIFbaqBzs3n7RXfxH2OHt4nn0BvSe-ug |
link.rule.ids | 315,786,790,4521,24144,27955,27956,45618,45712 |
linkProvider | Elsevier |
linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1bS8MwGP2Y24OCiFec1zz4WtZcesljmZPOzb44YW-haVOpSDfWCf58k6YFBfHB17Yp5SR830k55wTgjjEv456XOiSjzGFUuk6YY-kUKffzkJtNSJP2mfjxC3tcessejDsvjJFVtrXf1vSmWrdXRi2ao3VZjp6xptpYs2VCmhQVugMD5gWY9GEQTWdx0hXkwKjnzL5LP2_yEMPOQdfIvIq6fK1NbjchNvCU_96hvnWdh0M4aOkiiuwXHUFPVcewb_-1IWshOoFo8pkaRYvBGK0KZDSEnX4c2SOia6TJKbpPIqSL8caaGZCxlqAqLd_rU1g8TBbj2GmPRnAyvUfaOkTmiodMaTZnItUy3dUVTiUOlW43fspTluWYFa6eIFVgWVBJZeb63FdcZZrhnUG_WlXqHFDgy5y6hqWEBaMF5cZCpd8R6l5OMFVDcDo0xNoGYIhOGfYmLHrCoCdcLjR6Qwg6yMSPiRS6Rv858uLfI29hN148zcV8mswuYc_csYqTK-hvNx_qWvOGrbxp18UXXy-_Bw |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Examination+of+pretreatment+methods+for+DNA+extraction+from+nails&rft.jtitle=Forensic+science+international.+Genetics+supplement+series&rft.au=Ochiai%2C+Eriko&rft.au=Asogawa%2C+Minoru&rft.au=Irie%2C+Wataru&rft.au=Sasaki%2C+Chizuko&rft.date=2022-12-01&rft.pub=Elsevier+B.V&rft.issn=1875-1768&rft.eissn=1875-175X&rft.volume=8&rft.spage=110&rft.epage=111&rft_id=info:doi/10.1016%2Fj.fsigss.2022.09.039&rft.externalDocID=S1875176822000373 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1875-1768&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1875-1768&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1875-1768&client=summon |