Simultaneous quantitation of urine aldosterone and tetrahydroaldosterone in healthy Chinese subjects using a validated LC–MS/MS method
Aldosterone (ALD) is excreted in urine mainly as glucuronide conjugates of ALD and tetrahydroaldosterone. Measuring these urinary metabolites might be an alternative screening test to plasma ALD for primary aldosteronism. We report a validated LC–MS/MS method to measure both analytes simultaneously....
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| Published in | Biomedical chromatography Vol. 37; no. 9; pp. e5694 - n/a |
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| Main Authors | , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
England
01.09.2023
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| Abstract | Aldosterone (ALD) is excreted in urine mainly as glucuronide conjugates of ALD and tetrahydroaldosterone. Measuring these urinary metabolites might be an alternative screening test to plasma ALD for primary aldosteronism. We report a validated LC–MS/MS method to measure both analytes simultaneously. Urine samples underwent enzymatic hydrolysis to release the analytes from their glucuronide conjugates followed by organic solvent extraction and LC–MS/MS. The analytical performance of this method was evaluated. The within‐batch and between‐batch coefficients of variation for urine ALD and urine THA were all ≤5.2 and ≤3.7%. The lower limit of quantification was 0.5 nmol/L, and the linearity was up to at least 2770 nmol/L for both analytes. No significant matrix interference and carryover were observed. Both analytes in urine were stable for at least 48 h at 10°C and at least 18 months at −80°C. Local reference intervals were established from 126 healthy normotensive Chinese subjects (53% women, age: 20–65 years). Reference intervals for urine ALD and tetrahydroaldosterone were 2–38 and 9–139 nmol/day, respectively. This validated method can be applied to screening and diagnosing primary aldosteronism. |
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| AbstractList | Aldosterone (ALD) is excreted in urine mainly as glucuronide conjugates of ALD and tetrahydroaldosterone. Measuring these urinary metabolites might be an alternative screening test to plasma ALD for primary aldosteronism. We report a validated LC–MS/MS method to measure both analytes simultaneously. Urine samples underwent enzymatic hydrolysis to release the analytes from their glucuronide conjugates followed by organic solvent extraction and LC–MS/MS. The analytical performance of this method was evaluated. The within‐batch and between‐batch coefficients of variation for urine ALD and urine THA were all ≤5.2 and ≤3.7%. The lower limit of quantification was 0.5 nmol/L, and the linearity was up to at least 2770 nmol/L for both analytes. No significant matrix interference and carryover were observed. Both analytes in urine were stable for at least 48 h at 10°C and at least 18 months at −80°C. Local reference intervals were established from 126 healthy normotensive Chinese subjects (53% women, age: 20–65 years). Reference intervals for urine ALD and tetrahydroaldosterone were 2–38 and 9–139 nmol/day, respectively. This validated method can be applied to screening and diagnosing primary aldosteronism. Aldosterone (ALD) is excreted in urine mainly as glucuronide conjugates of ALD and tetrahydroaldosterone. Measuring these urinary metabolites might be an alternative screening test to plasma ALD for primary aldosteronism. We report a validated LC-MS/MS method to measure both analytes simultaneously. Urine samples underwent enzymatic hydrolysis to release the analytes from their glucuronide conjugates followed by organic solvent extraction and LC-MS/MS. The analytical performance of this method was evaluated. The within-batch and between-batch coefficients of variation for urine ALD and urine THA were all ≤5.2 and ≤3.7%. The lower limit of quantification was 0.5 nmol/L, and the linearity was up to at least 2770 nmol/L for both analytes. No significant matrix interference and carryover were observed. Both analytes in urine were stable for at least 48 h at 10°C and at least 18 months at -80°C. Local reference intervals were established from 126 healthy normotensive Chinese subjects (53% women, age: 20-65 years). Reference intervals for urine ALD and tetrahydroaldosterone were 2-38 and 9-139 nmol/day, respectively. This validated method can be applied to screening and diagnosing primary aldosteronism. Aldosterone (ALD) is excreted in urine mainly as glucuronide conjugates of ALD and tetrahydroaldosterone. Measuring these urinary metabolites might be an alternative screening test to plasma ALD for primary aldosteronism. We report a validated LC-MS/MS method to measure both analytes simultaneously. Urine samples underwent enzymatic hydrolysis to release the analytes from their glucuronide conjugates followed by organic solvent extraction and LC-MS/MS. The analytical performance of this method was evaluated. The within-batch and between-batch coefficients of variation for urine ALD and urine THA were all ≤5.2 and ≤3.7%. The lower limit of quantification was 0.5 nmol/L, and the linearity was up to at least 2770 nmol/L for both analytes. No significant matrix interference and carryover were observed. Both analytes in urine were stable for at least 48 h at 10°C and at least 18 months at -80°C. Local reference intervals were established from 126 healthy normotensive Chinese subjects (53% women, age: 20-65 years). Reference intervals for urine ALD and tetrahydroaldosterone were 2-38 and 9-139 nmol/day, respectively. This validated method can be applied to screening and diagnosing primary aldosteronism.Aldosterone (ALD) is excreted in urine mainly as glucuronide conjugates of ALD and tetrahydroaldosterone. Measuring these urinary metabolites might be an alternative screening test to plasma ALD for primary aldosteronism. We report a validated LC-MS/MS method to measure both analytes simultaneously. Urine samples underwent enzymatic hydrolysis to release the analytes from their glucuronide conjugates followed by organic solvent extraction and LC-MS/MS. The analytical performance of this method was evaluated. The within-batch and between-batch coefficients of variation for urine ALD and urine THA were all ≤5.2 and ≤3.7%. The lower limit of quantification was 0.5 nmol/L, and the linearity was up to at least 2770 nmol/L for both analytes. No significant matrix interference and carryover were observed. Both analytes in urine were stable for at least 48 h at 10°C and at least 18 months at -80°C. Local reference intervals were established from 126 healthy normotensive Chinese subjects (53% women, age: 20-65 years). Reference intervals for urine ALD and tetrahydroaldosterone were 2-38 and 9-139 nmol/day, respectively. This validated method can be applied to screening and diagnosing primary aldosteronism. |
| Author | Tsui, Teresa Kam Chi Lo, Clara Wai Shan Ho, Chung Shun Chan, Alan Shek Lun Luk, Yue Kin Cheng, Jenny Yeuk Ki |
| Author_xml | – sequence: 1 givenname: Jenny Yeuk Ki orcidid: 0009-0004-0805-2945 surname: Cheng fullname: Cheng, Jenny Yeuk Ki email: cyk033@ha.org.hk organization: Prince of Wales Hospital – sequence: 2 givenname: Clara Wai Shan surname: Lo fullname: Lo, Clara Wai Shan organization: Prince of Wales Hospital – sequence: 3 givenname: Alan Shek Lun surname: Chan fullname: Chan, Alan Shek Lun organization: Prince of Wales Hospital – sequence: 4 givenname: Yue Kin surname: Luk fullname: Luk, Yue Kin organization: Prince of Wales Hospital – sequence: 5 givenname: Teresa Kam Chi surname: Tsui fullname: Tsui, Teresa Kam Chi organization: Prince of Wales Hospital – sequence: 6 givenname: Chung Shun surname: Ho fullname: Ho, Chung Shun organization: The Chinese University of Hong Kong |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/37354001$$D View this record in MEDLINE/PubMed |
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| Snippet | Aldosterone (ALD) is excreted in urine mainly as glucuronide conjugates of ALD and tetrahydroaldosterone. Measuring these urinary metabolites might be an... |
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| SubjectTerms | aldosterone LC–MS/MS tetrahydroaldosterone |
| Title | Simultaneous quantitation of urine aldosterone and tetrahydroaldosterone in healthy Chinese subjects using a validated LC–MS/MS method |
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