Quantitation of amyloid beta peptides Aβ₁–₃₈, Aβ₁–₄₀, and Aβ₁–₄₂ in human cerebrospinal fluid by ultra-performance liquid chromatography–tandem mass spectrometry

• Published in: Analytical biochemistry Vol. 419; no. 2; pp. 133 - 139
• Main Authors: Lame, Mary E, Chambers, Erin E, Blatnik, Matthew
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 15.12.2011
• Subjects: Alzheimer disease; amyloid; antibodies; brain; cerebrospinal fluid; clinical trials; cross reaction; humans; immunoassay; immunoassays; ions; manufacturing; monitoring; peptides; quality control; rats; solid phase extraction; tandem mass spectrometry; ultra-performance liquid chromatography

Critical events in Alzheimer’s disease (AD) involve an imbalance between the production and clearance of amyloid beta (Aβ) peptides from the brain. Current methods for Aβ quantitation rely heavily on immuno-based techniques. However, these assays require highly specific antibodies and reagents that...