Shear Stress Inhibits Smooth Muscle Cell–Induced Inflammatory Gene Expression in Endothelial Cells: Role of NF-κB
OBJECTIVES—Vascular endothelial cells (ECs) are influenced by shear stress and neighboring smooth muscle cells (SMCs). We investigated the inflammation-relevant gene expression in EC/SMC cocultures under static condition and in response to shear stress. MATERIALS AND METHODS—Under static condition,...
Saved in:
Published in | Arteriosclerosis, thrombosis, and vascular biology Vol. 25; no. 5; pp. 963 - 969 |
---|---|
Main Authors | , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Philadelphia, PA
American Heart Association, Inc
01.05.2005
Hagerstown, MD Lippincott |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | OBJECTIVES—Vascular endothelial cells (ECs) are influenced by shear stress and neighboring smooth muscle cells (SMCs). We investigated the inflammation-relevant gene expression in EC/SMC cocultures under static condition and in response to shear stress.
MATERIALS AND METHODS—Under static condition, DNA microarrays and reverse-transcription polymerase chain reaction identified 23 inflammation-relevant genes in ECs whose expression was significantly affected by coculture with SMCs, with 18 upregulated and 5 downregulated. Application of shear stress (12 dynes/cm) to the EC side of the coculture for 6 hours inhibited most of the proinflammatory gene expressions in ECs induced by coculture with SMCs. Inhibition of nuclear factor-κB (NF-κB) activation by the p65-antisense, lactacystin, and N-acetyl-cysteine blocked the coculture-induced EC expression of proinflammatory genes, indicating that the NF-κB binding sites in the promoters of these genes play a significant role in their expression as a result of coculture with SMCs. Chromatin immunoprecipitation assays demonstrated the in vivo regulation of NF-κB recruitment to selected target promoters. Shear stress inhibited the SMC coculture-induced NF-κB activation in ECs and monocytic THP-1 cell adhesion to ECs.
CONCLUSIONS—Our findings suggest that shear stress plays an inhibitory role in the proinflammatory gene expression in ECs located in close proximity to SMCs. |
---|---|
AbstractList | Objectives—
Vascular endothelial cells (ECs) are influenced by shear stress and neighboring smooth muscle cells (SMCs). We investigated the inflammation-relevant gene expression in EC/SMC cocultures under static condition and in response to shear stress.
Materials and Methods—
Under static condition, DNA microarrays and reverse-transcription polymerase chain reaction identified 23 inflammation-relevant genes in ECs whose expression was significantly affected by coculture with SMCs, with 18 upregulated and 5 downregulated. Application of shear stress (12 dynes/cm
2
) to the EC side of the coculture for 6 hours inhibited most of the proinflammatory gene expressions in ECs induced by coculture with SMCs. Inhibition of nuclear factor-κB (NF-κB) activation by the p65-antisense, lactacystin, and N-acetyl-cysteine blocked the coculture-induced EC expression of proinflammatory genes, indicating that the NF-κB binding sites in the promoters of these genes play a significant role in their expression as a result of coculture with SMCs. Chromatin immunoprecipitation assays demonstrated the in vivo regulation of NF-κB recruitment to selected target promoters. Shear stress inhibited the SMC coculture-induced NF-κB activation in ECs and monocytic THP-1 cell adhesion to ECs.
Conclusions—
Our findings suggest that shear stress plays an inhibitory role in the proinflammatory gene expression in ECs located in close proximity to SMCs.
ECs are influenced by shear stress and SMCs. DNA microarrays showed increased proinflammatory gene expressions in ECs by static SMC coculture. Shear stress inhibits these coculture-induced expressions. NF-κB is involved in these coculture and shear stress modulations of gene expressions. Our results suggest shear stress as a protective regulator against inflammation. OBJECTIVES—Vascular endothelial cells (ECs) are influenced by shear stress and neighboring smooth muscle cells (SMCs). We investigated the inflammation-relevant gene expression in EC/SMC cocultures under static condition and in response to shear stress. MATERIALS AND METHODS—Under static condition, DNA microarrays and reverse-transcription polymerase chain reaction identified 23 inflammation-relevant genes in ECs whose expression was significantly affected by coculture with SMCs, with 18 upregulated and 5 downregulated. Application of shear stress (12 dynes/cm) to the EC side of the coculture for 6 hours inhibited most of the proinflammatory gene expressions in ECs induced by coculture with SMCs. Inhibition of nuclear factor-κB (NF-κB) activation by the p65-antisense, lactacystin, and N-acetyl-cysteine blocked the coculture-induced EC expression of proinflammatory genes, indicating that the NF-κB binding sites in the promoters of these genes play a significant role in their expression as a result of coculture with SMCs. Chromatin immunoprecipitation assays demonstrated the in vivo regulation of NF-κB recruitment to selected target promoters. Shear stress inhibited the SMC coculture-induced NF-κB activation in ECs and monocytic THP-1 cell adhesion to ECs. CONCLUSIONS—Our findings suggest that shear stress plays an inhibitory role in the proinflammatory gene expression in ECs located in close proximity to SMCs. |
Author | Hsieh, Hsing-Pang Usami, Shunichi Chang, Shun-Fu Lee, Ding-Yu Chien, Shu Lee, Chih-I Chiu, Jeng-Jiann Chen, Li-Jing Lee, Pei-Ling Tsai, Min-Chien |
AuthorAffiliation | From the Division of Medical Engineering Research (J.-J.C., L.-J.C., S.-F.C., P.-L.L., C.-I.L., M.-C.T., D.-Y.L.), National Health Research Institutes, Miaoli, Taiwan; the Institute of Biomedical Engineering (J.-J.C.), National Yang-Ming University, Taipei, Taiwan; and the Division of Biotechnology and Pharmaceutical Research (H.-P.H.), National Health Research Institutes, Miaoli, Taiwan; and the Departments of Bioengineering Medicine and Whitaker Institute of Biomedical Engineering (S.U., S.C.), University of California San Diego, La Jolla, Calif |
AuthorAffiliation_xml | – name: From the Division of Medical Engineering Research (J.-J.C., L.-J.C., S.-F.C., P.-L.L., C.-I.L., M.-C.T., D.-Y.L.), National Health Research Institutes, Miaoli, Taiwan; the Institute of Biomedical Engineering (J.-J.C.), National Yang-Ming University, Taipei, Taiwan; and the Division of Biotechnology and Pharmaceutical Research (H.-P.H.), National Health Research Institutes, Miaoli, Taiwan; and the Departments of Bioengineering Medicine and Whitaker Institute of Biomedical Engineering (S.U., S.C.), University of California San Diego, La Jolla, Calif |
Author_xml | – sequence: 1 givenname: Jeng-Jiann surname: Chiu fullname: Chiu, Jeng-Jiann organization: From the Division of Medical Engineering Research (J.-J.C., L.-J.C., S.-F.C., P.-L.L., C.-I.L., M.-C.T., D.-Y.L.), National Health Research Institutes, Miaoli, Taiwan; the Institute of Biomedical Engineering (J.-J.C.), National Yang-Ming University, Taipei, Taiwan; and the Division of Biotechnology and Pharmaceutical Research (H.-P.H.), National Health Research Institutes, Miaoli, Taiwan; and the Departments of Bioengineering Medicine and Whitaker Institute of Biomedical Engineering (S.U., S.C.), University of California San Diego, La Jolla, Calif – sequence: 2 givenname: Li-Jing surname: Chen fullname: Chen, Li-Jing – sequence: 3 givenname: Shun-Fu surname: Chang fullname: Chang, Shun-Fu – sequence: 4 givenname: Pei-Ling surname: Lee fullname: Lee, Pei-Ling – sequence: 5 givenname: Chih-I surname: Lee fullname: Lee, Chih-I – sequence: 6 givenname: Min-Chien surname: Tsai fullname: Tsai, Min-Chien – sequence: 7 givenname: Ding-Yu surname: Lee fullname: Lee, Ding-Yu – sequence: 8 givenname: Hsing-Pang surname: Hsieh fullname: Hsieh, Hsing-Pang – sequence: 9 givenname: Shunichi surname: Usami fullname: Usami, Shunichi – sequence: 10 givenname: Shu surname: Chien fullname: Chien, Shu |
BackLink | http://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16785407$$DView record in Pascal Francis |
BookMark | eNpFkMtOGzEUhq2KSuX2DlYlljM9Ht_G7CAKNBIXqYFuLePxKAOOHdkTUXZ9h75NH4KH4ElwEiSOF8eL__uP9B2gvRCDQ-g7gZoQQX4Aqc_uftdQhnAlgdaMUslqor6gfcIbVjFBxV75g1QVF6z5hg5yfix51jSwj8b5wpmE52NyOeNZWAwPw5jxfBnjuMDX62y9wxPn_dvff7PQra3rSqr3Zrk0Y0wv-NIFh6d_Vht-iAEPAU9DV2DnB-O3aD7Fv2KpiT2-uahe_58foa-98dkdf-xDdH8xvZv8rK5uL2eTs6vKNi3hFe2ASUstB9ZJJ7iQVDjVdLQzbSugb5XiXBjChVCtNL0T0D4oSS2VnTTU0UN0uuu1KeacXK9XaVia9KIJ6I0_DUQXf_rTn97600QV-GQHr0y2xvfJBDvkzwYhW85Alhzb5Z6jH13KT3797JIuWv242DQzKoBXDQAvD6Da3qLv6p2Dgg |
CODEN | ATVBFA |
Cites_doi | 10.1074/jbc.M307528200 10.1152/physrev.00041.2003 10.1093/hmg/7.6.1039 10.1161/atvb.18.1.75 10.1074/jbc.M314172200 10.1007/BF02584424 10.1016/S1357-2725(96)00159-8 10.1016/S0039-6060(98)70141-2 10.1161/res.88.6.615 10.1182/blood-2002-08-2560 10.1016/j.jbiomech.2003.08.012 10.3109/15419069809109147 10.1007/BF02634313 10.1006/jsre.1996.4978 10.1080/10623320210714 10.1161/01.cir.0000089373.49941.c4 10.1161/circ.103.4.597 10.1038/82176 |
ContentType | Journal Article |
Copyright | 2005 American Heart Association, Inc. 2005 INIST-CNRS |
Copyright_xml | – notice: 2005 American Heart Association, Inc. – notice: 2005 INIST-CNRS |
DBID | IQODW AAYXX CITATION |
DOI | 10.1161/01.ATV.0000159703.43374.19 |
DatabaseName | Pascal-Francis CrossRef |
DatabaseTitle | CrossRef |
DatabaseTitleList | CrossRef |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Medicine |
EISSN | 1524-4636 |
EndPage | 969 |
ExternalDocumentID | 10_1161_01_ATV_0000159703_43374_19 16785407 00043605-200505000-00015 |
GroupedDBID | - .Z2 01R 08R 0R 1J1 23N 2WC 3O- 40H 4Q1 4Q2 4Q3 53G 55 5GY 5RE 5VS 71W 77Y 7O 7O~ AAAXR AAMOA AAMTA AAPBV AARTV AAXQO ABBUW ABXVJ ABZAD ACDDN ACEWG ACGFS ACGOD ACPRK ACWDW ACWRI ACXNZ ADACO ADBBV ADFPA ADNKB AE3 AENEX AFFNX AFUWQ AHMBA AHULI AHVBC AIJEX AJIOK AJNYG AJYGW ALMA_UNASSIGNED_HOLDINGS AMJPA ASCII AWKKM BAWUL BOYCO C1A C45 CS3 DIK DUNZO E.X E3Z EBS EJD EX3 F2K F2L F2M F2N F5P FL- FRP FW0 GJ GX1 H0 H0~ H13 HZ IKYAY IN IN~ J5H JF9 JG8 JK3 JK8 K8S KD2 KMI KQ8 L-C L7B LI0 N9A N~7 N~B N~M O0- O9- OAG OAH OB2 OCUKA ODA OHASI OK1 OL1 OLG OLH OLU OLV OLW OLY OLZ OPUJH ORVUJ OUVQU OVD OVDNE OVIDH OVLEI OWW OWY OXXIT P-K P2P PQEST PQQKQ PZZ RAH RHF RIG RLZ RSW S4R S4S V2I WOQ WOW X3V X3W X7M Z2 ZA5 ZGI --- .3C .55 .GJ 0R~ AAGIX AAHPQ AAQKA AASOK AAUGY ABASU ABDIG ABQRW ACCJW ACILI ADGGA AE6 AEETU AFDTB AGINI AHJKT AHOMT AHRYX AJNWD AKALU AKULP ALMTX AMKUR AMNEI AOHHW AYCSE BS7 DIWNM EEVPB FCALG GNXGY GQDEL HZ~ IKREB IPNFZ IQODW OJAPA OWU OWV OWX OWZ T8P TEORI TR2 TSPGW VVN W3M W8F XXN XYM YFH ZZMQN AAAAV AAIQE AASCR AAYXX ABJNI ABVCZ ACXJB ADHPY AHQNM AINUH AJZMW CITATION ERAAH HLJTE |
ID | FETCH-LOGICAL-c2815-3d047c3c504d7e656736e92d3da8860f899556a1566987afe608b973c37d7a3e3 |
ISSN | 1079-5642 |
IngestDate | Fri Aug 23 00:17:19 EDT 2024 Sun Oct 22 16:07:59 EDT 2023 Thu Aug 13 19:50:12 EDT 2020 |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 5 |
Keywords | Endothelial cell endothelial cells Cardiovascular disease Smooth muscle cDNA microarray Gene expression Vascular disease smooth muscle cells coculture Complementary DNA Atherosclerosis Shear stress Inflammatory cell |
Language | English |
License | CC BY 4.0 |
LinkModel | OpenURL |
MergedId | FETCHMERGED-LOGICAL-c2815-3d047c3c504d7e656736e92d3da8860f899556a1566987afe608b973c37d7a3e3 |
PageCount | 7 |
ParticipantIDs | crossref_primary_10_1161_01_ATV_0000159703_43374_19 pascalfrancis_primary_16785407 wolterskluwer_health_00043605-200505000-00015 |
ProviderPackageCode | L-C C45 7O~ AARTV ADFPA OLH ASCII OLG AAMOA ODA ABZAD ABBUW JK3 ADNKB JK8 H0~ 1J1 OLV OLU JG8 OLW OLZ OLY F2K F2M F2L F2N OHASI AHVBC AJNYG FL- KMI K8S OVLEI AJIOK OPUJH V2I S4R S4S 4Q1 DUNZO OAG 4Q2 OVDNE 4Q3 AMJPA OAH OVD 71W AHULI OB2 ACEWG .Z2 N~7 IKYAY OVIDH AWKKM 40H N~B OUVQU ORVUJ X3V X3W ACDDN ACWRI BOYCO AIJEX AAXQO AAMTA AAAXR E.X OWW OCUKA OWY 01R ACXNZ OL1 ABXVJ IN~ KD2 OXXIT 77Y ACWDW JF9 FW0 |
PublicationCentury | 2000 |
PublicationDate | 2005-May |
PublicationDateYYYYMMDD | 2005-05-01 |
PublicationDate_xml | – month: 05 year: 2005 text: 2005-May |
PublicationDecade | 2000 |
PublicationPlace | Philadelphia, PA Hagerstown, MD |
PublicationPlace_xml | – name: Philadelphia, PA – name: Hagerstown, MD |
PublicationTitle | Arteriosclerosis, thrombosis, and vascular biology |
PublicationYear | 2005 |
Publisher | American Heart Association, Inc Lippincott |
Publisher_xml | – name: American Heart Association, Inc – name: Lippincott |
References | e_1_3_2_9_2 e_1_3_2_15_2 e_1_3_2_8_2 e_1_3_2_16_2 e_1_3_2_7_2 e_1_3_2_17_2 e_1_3_2_6_2 e_1_3_2_18_2 e_1_3_2_1_2 e_1_3_2_10_2 e_1_3_2_5_2 e_1_3_2_11_2 e_1_3_2_4_2 e_1_3_2_12_2 e_1_3_2_3_2 e_1_3_2_13_2 e_1_3_2_2_2 e_1_3_2_14_2 |
References_xml | – ident: e_1_3_2_11_2 doi: 10.1074/jbc.M307528200 – ident: e_1_3_2_15_2 doi: 10.1152/physrev.00041.2003 – ident: e_1_3_2_13_2 doi: 10.1093/hmg/7.6.1039 – ident: e_1_3_2_4_2 doi: 10.1161/atvb.18.1.75 – ident: e_1_3_2_18_2 doi: 10.1074/jbc.M314172200 – ident: e_1_3_2_1_2 doi: 10.1007/BF02584424 – ident: e_1_3_2_10_2 doi: 10.1016/S1357-2725(96)00159-8 – ident: e_1_3_2_6_2 doi: 10.1016/S0039-6060(98)70141-2 – ident: e_1_3_2_7_2 doi: 10.1161/res.88.6.615 – ident: e_1_3_2_8_2 doi: 10.1182/blood-2002-08-2560 – ident: e_1_3_2_9_2 doi: 10.1016/j.jbiomech.2003.08.012 – ident: e_1_3_2_14_2 doi: 10.3109/15419069809109147 – ident: e_1_3_2_3_2 doi: 10.1007/BF02634313 – ident: e_1_3_2_16_2 doi: 10.1006/jsre.1996.4978 – ident: e_1_3_2_2_2 doi: 10.1080/10623320210714 – ident: e_1_3_2_17_2 doi: 10.1161/01.cir.0000089373.49941.c4 – ident: e_1_3_2_5_2 doi: 10.1161/circ.103.4.597 – ident: e_1_3_2_12_2 doi: 10.1038/82176 |
SSID | ssj0004220 |
Score | 2.1021307 |
Snippet | OBJECTIVES—Vascular endothelial cells (ECs) are influenced by shear stress and neighboring smooth muscle cells (SMCs). We investigated the... Objectives— Vascular endothelial cells (ECs) are influenced by shear stress and neighboring smooth muscle cells (SMCs). We investigated the... |
SourceID | crossref pascalfrancis wolterskluwer |
SourceType | Aggregation Database Index Database Publisher |
StartPage | 963 |
SubjectTerms | Atherosclerosis (general aspects, experimental research) Biological and medical sciences Blood and lymphatic vessels Blood vessels and receptors Cardiology. Vascular system Diseases of the peripheral vessels. Diseases of the vena cava. Miscellaneous Fundamental and applied biological sciences. Psychology Medical sciences Vertebrates: cardiovascular system |
Title | Shear Stress Inhibits Smooth Muscle Cell–Induced Inflammatory Gene Expression in Endothelial Cells: Role of NF-κB |
URI | http://ovidsp.ovid.com/ovidweb.cgi?T=JS&NEWS=n&CSC=Y&PAGE=fulltext&D=ovft&AN=00043605-200505000-00015 |
Volume | 25 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1bb9MwFLbKkBASQlxFuUx-YE-TSxInvvDWTq3K2CoBLdpblIuzVazpRBoh8dP4Ebzxfzi-pEnREJeXqHEuVny-HtvH3_mM0MtMgctTEXg_JjwSslSTAERBsiKXoc6FjExS2OmMTRfh8Vl01uv96LCW6k06yL5em1fyP1aFMrCrzpL9B8tuXwoF8BvsC0ewMBz_ysZmO-om3WNZXixTvQxQrdbQ_IeruoLbD3VknsDEu9YL_VApIGBlV9bhtUor_FsmrGE8qjLXGVmXOoyuH6xMxOC9YyDOJuTgaHwwGo66Q9qhJoUu17oy6HGtZIHefGGVNmc6Nr9lvDrVp-3ax_TNwrFszskxYLVsr4xnNmoA5a5_NcVDm2b14aIuyaRuyk_GY8s3XpKT5vYmmhG13EEbzdiqUnR8sscliZgV4Roo56eDkGits64jtxnUDrBRxytL50OVO5PX9x3MN_kQg-H8o5G1hJEe-MNBSCkPB86x7wh2_9KRbumNPowAtLDhDXQz4DLSTNO37zoi9kFgRTLcdzktXKj_1e9r3xk33bkCoyWXhd17Bc6_rDW3ovpkUis6A6T5PXTXzWzw0ML0Puqp8gG6deq4Gw9RZdCKLVpxg1Zs0YotWnEXrbiLVqzRilu0wkXcQat5sMKvscYqXhcYsPr92-gRWkzG86MpcVt-kCwQfkRo7oU8o1nkhTlXMNfglCkZ5DRPhGBeISQ0J0t00EEKnhSKeSKVnGaU5zyhij5Ge-W6VE8QllzkTHG4V6YhD5JUFioVVEtsBrkqRB_RpkHjK6vsEpsZMfNjz4_BDHFrhtiYIfZlH-3vtH37qLN5H5EdY8Q2nzk2a-4M0B6YzSOdoIMfPf3TC5-h2-0f5Tna23yu1QsYCG_SfQOrn0hvrZU |
link.rule.ids | 315,786,790,27955,27956 |
linkProvider | Colorado Alliance of Research Libraries |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Shear+stress+inhibits+smooth+muscle+cell-induced+inflammatory+gene+expression+in+endothelial+cells+%3A+Role+of+NF-%CE%BAB&rft.jtitle=Arteriosclerosis%2C+thrombosis%2C+and+vascular+biology&rft.au=CHIU%2C+Jeng-Jiann&rft.au=CHEN%2C+Li-Jing&rft.au=CHANG%2C+Shun-Fu&rft.au=LEE%2C+Pei-Ling&rft.date=2005-05-01&rft.pub=Lippincott&rft.issn=1079-5642&rft.eissn=1524-4636&rft.volume=25&rft.issue=5&rft.spage=963&rft.epage=969&rft_id=info:doi/10.1161%2F01.ATV.0000159703.43374.19&rft.externalDBID=n%2Fa&rft.externalDocID=16785407 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1079-5642&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1079-5642&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1079-5642&client=summon |