Simultaneous Extraction of Bone Marrow RNA and DNA from Patients with Hematologic Diseases Using a Combined Magnetic Bead Method within 1 Hour

• Published in: The journal of applied laboratory medicine Vol. 9; no. 6; p. 1004
• Main Authors: Mu, Huijun, Zou, Jian, Zhang, Haiping
• Format: Journal Article
• Language: English
• Published: England 04.11.2024
• Subjects: Adult; Bone Marrow; DNA - genetics; DNA - isolation & purification; Female; Guanidines; Hematologic Diseases - genetics; Hematologic Diseases - therapy; Humans; Male; Middle Aged; Phenols; RNA - genetics; RNA - isolation & purification

TRIzolTM is widely used for RNA and DNA extraction. However, this method is laborious and time-consuming. The objective of this study was to validate a time-effective and labor-saving protocol. The TRIzol method was used to separate the aqueous phase, protein, and phenol layer of bone marrow samples...