DNA Methylation Profiling of Heterogeneous Sporadic LAM and Matched Lung Tissue

• Published in: American journal of respiratory cell and molecular biology Vol. 70; no. 1; pp. 81 - 84
• Main Authors: Powell, Ryan M, Moravec, Jiří C, Jones, Greg T, Bhat, Basharat, Lin, Susan M, Planer, Joseph D, Krymskaya, Vera P, Cantu, Edward, Pattison, Sharon, Morison, Ian M, Gray, Bronwyn, Eccles, Michael R, Macaulay, Erin C
• Format: Journal Article
• Language: English
• Published: United States American Thoracic Society 01.01.2024
• Subjects: Cell growth; Cell proliferation; CpG islands; Deoxyribonucleic acid; DNA; DNA fingerprinting; DNA methylation; DNA probes; Gene silencing; Genomes; Lungs; Respiratory system; Tissues; Tumorigenesis
• ISSN: 1044-1549; 1535-4989
• DOI: 10.1165/rcmb.2023-0300LE

Powell et al discuss DNA methylation Pprofiling of heterogeneous sporadic LAM and matched lung tissue. They investigate the DNA methylation profiles of heterogeneous sporadic lymphangioleiomyomatosis (SLAM) tissue and matched control lung tissue. SLAM is a rare neoplasm characterized by the invasion and proliferation of cells within the lungs. The researchers used DNA methylation as a biomarker to identify the tissue of origin for SLAM. They generated genome-wide DNA methylation profiles from SLAM samples and control lung samples and identified 4932 differentially methylated probes (DMPs) between the two groups. The DMPs were found to be associated with CpG islands and the first exon of genes, suggesting gene silencing in tumorigenesis. The DMPs also showed associations with FOX transcription factor binding motifs. In addition, the researchers performed in silico deconvolution to isolate a pure SLAM methylome and found that it clustered closely with ovarian and uterine samples.