Relation between Structure and Anti-viral Activity in Interferon System

• Published in: YAKUGAKU ZASSHI Vol. 115; no. 5; pp. 390 - 393
• Main Authors: SUGIYAMA, Kenji, VOSS, Tilman, AHORN, Horst, FOGY, Ingrid MAURER, BARSOUMIAN, Edward L.
• Format: Journal Article
• Language: English; Japanese
• Published: Japan The Pharmaceutical Society of Japan 01.05.1995
• Subjects: Animals; anti-viral activity; CHO Cells; Cricetinae; Encephalomyocarditis virus - drug effects; Escherichia coli; Female; Glycosylation; Humans; IFN-α2; IFN-ω1; Interferon alpha-2; Interferon Type I - chemistry; Interferon Type I - pharmacology; Interferon-alpha - chemistry; Interferon-alpha - pharmacology; Mice; Recombinant Proteins - chemistry; Recombinant Proteins - pharmacology; Spodoptera; Structure-Activity Relationship
• ISSN: 0031-6903; 1347-5231
• DOI: 10.1248/yakushi1947.115.5_390

The interferons (IFN) were discovered in 1957 as biological agents interfering with viral replication. IFNs were initially classified according to their sources as leukocyte, fibroblast and immune IFNs. Both leukocyte and fibroblast IFNs are designated as Type I IFNs and immune IFN as a Type II IFN. There are almost twenty related IFNs which belong to the group of Type I IFN. IFN-α2 and IFN-ω1 are described as members of the large group of Type I IFN. Both natural IFN-α2 and IFN-ω1 are isolated from human leukocytes. IFN-α2 carries an O-linked carbohydrate moiety, whereas IFN-ω1 has an N-linked complex oligosaccharide. Recombinant IFN-α2 expressed in E coli. and Sf-9 cells have been characterized. Moreover, recombinant IFN-ω1 expressed in CHO cells and Sf-9 cells have also been characterized. In the present study, the recombinant human IFN-α2 and IFN-ω1 were analyzed for their anti-viral activity compared with native products.