Mucosal enzymes in human inflammatory bowel disease with reference to neutrophil granulocytes as mediators of tissue injury
1. Biopsies of rectal mucosa were obtained for histology and enzyme analysis from 32 patients with inflammatory and functional bowel disorders, and the biopsies were classified morphologically as active colitis, quiescent colitis or normal. 2. Supernatant fractions of biopsy homogenates were assayed...
Saved in:
Published in | Clinical science (1979) Vol. 57; no. 4; pp. 295 - 303 |
---|---|
Main Authors | , |
Format | Journal Article |
Language | English |
Published |
England
01.10.1979
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | 1. Biopsies of rectal mucosa were obtained for histology and enzyme analysis from 32 patients with inflammatory and functional bowel disorders, and the biopsies were classified morphologically as active colitis, quiescent colitis or normal. 2. Supernatant fractions of biopsy homogenates were assayed for their content of the proteolytic enzymes alpha-chymotrypsin, elastase and cathepsin D, and of protein, unsaturated vitamin B12-binding capacity, lysozyme, myeloperoxidase and N-acetyl-beta-glucosaminidase. 3. Mean unsaturated vitamin B12-binding capacity was significantly raised above normal in the active colitic mucosa, and mean lysozyme activity was raised above normal in both active and quiescent mucosae. 4. In active colitic mucosa there was no rise above normal in mean activities of any of the proteolytic enzymes, though a significant fall below normal occurred in mean N-acetyl-beta-glucosaminidase activity in the active colitic group. |
---|---|
AbstractList | 1. Biopsies of rectal mucosa were obtained for histology and enzyme analysis from 32 patients with inflammatory and functional bowel disorders, and the biopsies were classified morphologically as active colitis, quiescent colitis or normal.
2. Supernatant fractions of biopsy homogenates were assayed for their content of the proteolytic enzymes α-chymotrypsin, elastase and cathepsin D, and of protein, unsaturated vitamin B12-binding capacity, lysozyme, myeloperoxidase and N-acetyl-β-glucosaminidase.
3. Mean unsaturated vitamin B12-binding capacity was significantly raised above normal in the active colitic mucosa, and mean lysozyme activity was raised above normal in both active and quiescent mucosae.
4. In active colitic mucosa there was no rise above normal in mean activities of any of the proteolytic enzymes, though a significant fall below normal occurred in mean N-acetyl-β-glucosaminidase activity in the active colitic group. 1. Biopsies of rectal mucosa were obtained for histology and enzyme analysis from 32 patients with inflammatory and functional bowel disorders, and the biopsies were classified morphologically as active colitis, quiescent colitis or normal. 2. Supernatant fractions of biopsy homogenates were assayed for their content of the proteolytic enzymes alpha-chymotrypsin, elastase and cathepsin D, and of protein, unsaturated vitamin B12-binding capacity, lysozyme, myeloperoxidase and N-acetyl-beta-glucosaminidase. 3. Mean unsaturated vitamin B12-binding capacity was significantly raised above normal in the active colitic mucosa, and mean lysozyme activity was raised above normal in both active and quiescent mucosae. 4. In active colitic mucosa there was no rise above normal in mean activities of any of the proteolytic enzymes, though a significant fall below normal occurred in mean N-acetyl-beta-glucosaminidase activity in the active colitic group. |
Author | Vincenti, A C Kane, S P |
Author_xml | – sequence: 1 givenname: S P surname: Kane fullname: Kane, S P – sequence: 2 givenname: A C surname: Vincenti fullname: Vincenti, A C |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/228886$$D View this record in MEDLINE/PubMed |
BookMark | eNo9kDtPwzAUhS1UHuUxsDN4QmII-JHEzogQL6mIBebIcW9oUGIX31hV4M9jVFTd4dzh0yedc0xmzjsg5Jyza85ycWORFYqJqtgjc54rlmklyxmZM57LrBCCH5FjxE_GhEx3SA6E0FqXc_LzEq1H01Nw39MASDtHV3EwLj1tb4bBjD5MtPEb6OmyQzAIdNONKxqghQDOAh09dRDH4NerrqcfwbjYezuNyWaQDrDs_iRIfUvHDjFCcn_GMJ2S_db0CGf_eULeH-7f7p6yxevj893tIrNCVWNWtqqynPElbypZaFXkjayU4GUjl9qqnHPLC1sWpRZa2FSvEGBlw5lmDCot5Am53HrXwX9FwLEeOrTQ98aBj1irXFWq4iyBV1vQBo-YCtbr0A0mTDVn9d_O9W7nxF78S2OTKu7I7bDyFyvAey4 |
CitedBy_id | crossref_primary_10_1136_jcp_34_1_1 crossref_primary_10_1002__SICI_1520_6823_1997_5_6_275__AID_ROI3_3_0_CO_2_V crossref_primary_10_1016_0022_4804_92_90053_3 crossref_primary_10_1007_BF02552623 crossref_primary_10_1136_gut_33_2_242 crossref_primary_10_3109_00365528608996396 crossref_primary_10_1136_gut_25_5_455 crossref_primary_10_1136_gut_31_12_1358 crossref_primary_10_1016_0016_5085_89_90631_8 crossref_primary_10_1136_gut_26_12_1306 crossref_primary_10_1080_00365529850166248 crossref_primary_10_1097_00004836_200009000_00013 crossref_primary_10_1016_0002_9343_89_90230_1 crossref_primary_10_1136_gut_26_8_761 crossref_primary_10_3109_00365529008998554 crossref_primary_10_1136_jcp_36_11_1312 crossref_primary_10_3109_00365528708991488 |
ContentType | Journal Article |
DBID | CGR CUY CVF ECM EIF NPM AAYXX CITATION 7X8 |
DOI | 10.1042/cs0570295 |
DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed CrossRef MEDLINE - Academic |
DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) CrossRef MEDLINE - Academic |
DatabaseTitleList | CrossRef MEDLINE MEDLINE - Academic |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Medicine |
EISSN | 1470-8736 |
EndPage | 303 |
ExternalDocumentID | 10_1042_cs0570295 228886 |
Genre | Journal Article |
GroupedDBID | --- .55 .GJ 0R~ 0VX 29B 2WC 3O- 4.4 53G 5GY 5RE 5VS 6J9 AABGO AAHRG ABCQX ABJNI ACGFO ACGFS ADBBV AEGXH AENEX AFFNX AFFVI AI. AIAGR AIZAD ALMA_UNASSIGNED_HOLDINGS BAWUL CGR CS3 CUY CVF DU5 E3Z EBD EBS ECM EIF EJD EMOBN F5P GX1 H13 HZ~ L7B MV1 MVM NPM NTEUP O9- OHT P2P P6G RHI RPO SV3 TR2 VH1 WH7 X7M ZGI ZXP AAYXX CITATION 7X8 |
ID | FETCH-LOGICAL-c279t-6f79c101d1b9358754b397216b3d8c7411c15c6568282c23252ec3b10800e9823 |
ISSN | 0143-5221 |
IngestDate | Sat Aug 17 00:49:18 EDT 2024 Fri Aug 23 00:26:13 EDT 2024 Sat Sep 28 08:35:08 EDT 2024 |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 4 |
Language | English |
LinkModel | OpenURL |
MergedId | FETCHMERGED-LOGICAL-c279t-6f79c101d1b9358754b397216b3d8c7411c15c6568282c23252ec3b10800e9823 |
Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
PMID | 228886 |
PQID | 74797910 |
PQPubID | 23479 |
PageCount | 9 |
ParticipantIDs | proquest_miscellaneous_74797910 crossref_primary_10_1042_cs0570295 pubmed_primary_228886 |
PublicationCentury | 1900 |
PublicationDate | 1979-Oct |
PublicationDateYYYYMMDD | 1979-10-01 |
PublicationDate_xml | – month: 10 year: 1979 text: 1979-Oct |
PublicationDecade | 1970 |
PublicationPlace | England |
PublicationPlace_xml | – name: England |
PublicationTitle | Clinical science (1979) |
PublicationTitleAlternate | Clin Sci (Lond) |
PublicationYear | 1979 |
SSID | ssj0023232 |
Score | 1.3115077 |
Snippet | 1. Biopsies of rectal mucosa were obtained for histology and enzyme analysis from 32 patients with inflammatory and functional bowel disorders, and the... |
SourceID | proquest crossref pubmed |
SourceType | Aggregation Database Index Database |
StartPage | 295 |
SubjectTerms | Acetylglucosaminidase - metabolism Adolescent Adult Aged Carrier Proteins - metabolism Cathepsins - metabolism Chymotrypsin - metabolism Colitis, Ulcerative - enzymology Crohn Disease - enzymology Female Humans Intestinal Mucosa - enzymology Male Middle Aged Muramidase - metabolism Neutrophils - enzymology Pancreatic Elastase - metabolism Peroxidase - metabolism Vitamin B 12 - metabolism |
Title | Mucosal enzymes in human inflammatory bowel disease with reference to neutrophil granulocytes as mediators of tissue injury |
URI | https://www.ncbi.nlm.nih.gov/pubmed/228886 https://search.proquest.com/docview/74797910 |
Volume | 57 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3da9RAEF-0gvgiflQ8Pwfx7UhMdjeX5LEe2qq0CF7l3kJ2b0NPbFJ6CdL6zzv7kU3OKlRfwrGwCzfzY3Y-fjNLyGvBKt2vSQOhVBVwrhNNnJVBJJMs52W5ktJM-zyaHRzzj8tkOVCHTHdJK0J5-ce-kv_RKq6hXnWX7D9o1h-KC_gb9Ytf1DB-r6XjQ00314OB68uLU8Osckn5D3WFmj61FfS3zQ-lbZupxNjE6zBeFl3PI9W1583Zyfr7dB9vrg6vtwudjS03poxTmvd40KdcGB3h2d-67T7q-VZ3pTRea5yn-SjL8MlRar-E089hv_h1XWtuqOET7IXTeTikIPR2T2YbspIMI1rb6hwqa0l5GqGptdNNelNrZ1E7SPGx3bQvbbormJmxB1etOxoYVIncoI8Z-R3jCdq_3Wyeb2gq7ZwWfutNcoumeaI5oPtLzwlC79K8aOf_Tz-KitM3fuu2A_OXqMR4J4t75K4LK2DPYuQ-uaHqB-T2oSNOPCQ_HVTAQQXWNRiowBgqYKACDiqgoQIeKtA2MEAFxlCBcgMeKtBUYKECFiq75Pj9u8X8IHDvbgQShdIGsyrNJZrqVSx0lTxNuGBmyJNgq0yiCxrLOJEYCGC0TiWKLKFKMqHZqpHKM8oekZ26qdVjAnr0Us5msWBS8DJi2ixkq4RRgX5mLtMJedULsziz41WKK8qakJe9mAs0frqihaBtuk2BsTDCMY4mZNdK3x9CaZZlsyfXOf4puTOA-hnZac879Rw9zVa8MOj4BYuFfGY |
link.rule.ids | 315,786,790,27955,27956 |
linkProvider | Geneva Foundation for Medical Education and Research |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Mucosal+Enzymes+in+Human+Inflammatory+Bowel+Disease+with+Reference+to+Neutrophil+Granulocytes+as+Mediators+of+Tissue+Injury&rft.jtitle=Clinical+science+%281979%29&rft.au=Kane%2C+S.+P.&rft.au=Vincenti%2C+A.+C.&rft.date=1979-10-01&rft.issn=0143-5221&rft.eissn=1470-8736&rft.volume=57&rft.issue=4&rft.spage=295&rft.epage=303&rft_id=info:doi/10.1042%2Fcs0570295&rft.externalDBID=n%2Fa&rft.externalDocID=10_1042_cs0570295 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0143-5221&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0143-5221&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0143-5221&client=summon |