Purification, sequence and crystallization of an anti-tissue factor Fab and its use for the crystallization of tissue factor

• Published in: Journal of crystal growth Vol. 122; no. 1; pp. 253 - 264
• Main Authors: Ruf, Wolfram, Stura, Enrico A., LaPolla, Robert J., Syed, Rashid, Edgington, Thomas S., Wilson, Ian A.
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Amsterdam Elsevier B.V 01.01.1992; Elsevier
• Subjects: Antigen-antibody reactions, antigen-antibody complexes, antibody-complement and others. Study of affinity. Antigen presentation; Biological and medical sciences; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Molecular immunology; Crystal growth; Purification; Nucleotide sequence; Peptide fragment; Tissue factor; Monoclonal antibody; Aminoacid sequence; Immune complex

The crystallization of proteins as Fab-antigen complexes may be advantageous in the crystallization of biologically important proteins, since many different monoclonal antibodies are often available against a given protein. From each of these, Fab fragments can be generated providing new opportuniti...