Determination of L-ascorbic acid, tocopherol, carotene, and chlorophyll in various teas
L-Ascorbic aid, tocopherol, carotene and chlorophyll contents of various teas were determined by high performance liquid chromatography. Ascorbic acid was measured on Inertsil ODS-2 with amperometer at+500mV vs Ag/AgC1. Tocopherols were extracted from the sample with hexane and pretreated using flor...
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| Published in | NIPPON SHOKUHIN KOGYO GAKKAISHI Vol. 36; no. 11; pp. 927 - 933 |
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| Main Authors | , , , , , , |
| Format | Journal Article |
| Language | English Japanese |
| Published |
Japanese Society for Food Science and Technology
1989
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| Subjects | |
| Online Access | Get full text |
| ISSN | 0029-0394 |
| DOI | 10.3136/nskkk1962.36.11_927 |
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| Abstract | L-Ascorbic aid, tocopherol, carotene and chlorophyll contents of various teas were determined by high performance liquid chromatography. Ascorbic acid was measured on Inertsil ODS-2 with amperometer at+500mV vs Ag/AgC1. Tocopherols were extracted from the sample with hexane and pretreated using florisil mini column, and determined on LiChrosorb NH2 With a flouorescence detection. Carotene and chlorophylls were separated on Zorbax ODS and detected with visible wavelength detector. All kinds of those compounds examined were found much more in sencha than semi-fermented (Oolong) and fermented (Black) teas. The average contents of ascorbic acid, a-tocopherol, a and β-carotene in sencha were 167, 13.5, 3.0 and 40.2mg/100g, respectively. In sencha, carotenes(α+β; X) were correlated With chlorophylls (a+b; Y): Y= 6.73 X-86.5, r=0.912. Those compounds in the solutions decocted from teas by usual way were also determined. These compounds except ascorbic acid did not detected. |
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| AbstractList | L-Ascorbic aid, tocopherol, carotene and chlorophyll contents of various teas were determined by high performance liquid chromatography. Ascorbic acid was measured on Inertsil ODS-2 with amperometer at+500mV vs Ag/AgC1. Tocopherols were extracted from the sample with hexane and pretreated using florisil mini column, and determined on LiChrosorb NH2 With a flouorescence detection. Carotene and chlorophylls were separated on Zorbax ODS and detected with visible wavelength detector. All kinds of those compounds examined were found much more in sencha than semi-fermented (Oolong) and fermented (Black) teas. The average contents of ascorbic acid, a-tocopherol, a and β-carotene in sencha were 167, 13.5, 3.0 and 40.2mg/100g, respectively. In sencha, carotenes(α+β; X) were correlated With chlorophylls (a+b; Y): Y= 6.73 X-86.5, r=0.912. Those compounds in the solutions decocted from teas by usual way were also determined. These compounds except ascorbic acid did not detected. |
| Author | Tamase, K Yamamoto, M Sasaki, M Maeda, Y Yonetani, T Yamazoe, Y Kitada, Y. (Nara-ken. Inst. of Public Health (Japan)) |
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| References | 13) NELLS, H.J.C.F. and DELEENHEER, A.P.:Ana1. Chem., 55, 270 (1983). 4) KITADA, Y., TAMASE, K., SASAKI, M. and YAMAZOE, Y.: Nippon Shokuhin Kogyo Gakkaishi, 36, 592 (1989). 11) 山内亮・松下雪郎: 農化, 50, 525 (1976). 12) HSIEH, Y.C.: J. Chromatogr., 259, 515 (1983). 2) 中川致之: 食の科学, 28, 56 (1976). 15) 池ケ谷賢次郎・高柳博次・阿南豊正: 日食工誌, 31, 459 (1984). 1) GEY, K.F., STAHELIN, H.B. and BRUBACHER, G.B.: The 4th Biennial General Meeting of the Society for Free Radical Research, p.28, Kyoto (1988). 14) 山内亮・松下雪郎:農化, 50, 569 (1976). 10) 日本薬学会編: 衛生試験法・注解(金原出版,東京), p.1323 (1986). 8) WILSON, C.W, and SHAW, P.E.: J. Agric. Food Chem., 35, 329 (1987). 6) SHAW, P.E. and WILSGN, C.W.: J. Agric. Food Chem., 30, 396 (1982). 16) 河村真也・故倉宏至・松村康生・森友彦:農化, 61, 1567 (1987). 5) CARR, R.S. and NEFF, J.M.: Anal. Chem. 52, 2428 (1980). 9) 兼松弘・牛草寿昭・丸出武紀・新谷勲・松本太郎: 油化学. 32, 51 (1983). 3) 井崎やゑ子・吉田宏三・日高公雄・戸田和子: 栄食誌, 39, 485 (1986). 7) ASHOOR, S.H., MONFG, W.C. and WELTY, J.:J. Assoc. Off. Ana1. Chem., 67, 78 (1984). |
| References_xml | – reference: 4) KITADA, Y., TAMASE, K., SASAKI, M. and YAMAZOE, Y.: Nippon Shokuhin Kogyo Gakkaishi, 36, 592 (1989). – reference: 16) 河村真也・故倉宏至・松村康生・森友彦:農化, 61, 1567 (1987). – reference: 11) 山内亮・松下雪郎: 農化, 50, 525 (1976). – reference: 14) 山内亮・松下雪郎:農化, 50, 569 (1976). – reference: 8) WILSON, C.W, and SHAW, P.E.: J. Agric. Food Chem., 35, 329 (1987). – reference: 9) 兼松弘・牛草寿昭・丸出武紀・新谷勲・松本太郎: 油化学. 32, 51 (1983). – reference: 6) SHAW, P.E. and WILSGN, C.W.: J. Agric. Food Chem., 30, 396 (1982). – reference: 7) ASHOOR, S.H., MONFG, W.C. and WELTY, J.:J. Assoc. Off. Ana1. Chem., 67, 78 (1984). – reference: 5) CARR, R.S. and NEFF, J.M.: Anal. Chem. 52, 2428 (1980). – reference: 10) 日本薬学会編: 衛生試験法・注解(金原出版,東京), p.1323 (1986). – reference: 15) 池ケ谷賢次郎・高柳博次・阿南豊正: 日食工誌, 31, 459 (1984). – reference: 13) NELLS, H.J.C.F. and DELEENHEER, A.P.:Ana1. Chem., 55, 270 (1983). – reference: 12) HSIEH, Y.C.: J. Chromatogr., 259, 515 (1983). – reference: 1) GEY, K.F., STAHELIN, H.B. and BRUBACHER, G.B.: The 4th Biennial General Meeting of the Society for Free Radical Research, p.28, Kyoto (1988). – reference: 2) 中川致之: 食の科学, 28, 56 (1976). – reference: 3) 井崎やゑ子・吉田宏三・日高公雄・戸田和子: 栄食誌, 39, 485 (1986). |
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| Title | Determination of L-ascorbic acid, tocopherol, carotene, and chlorophyll in various teas |
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