Functional Characterization of a Higher Plant Sphingolipid Δ4-Desaturase: Defining the Role of Sphingosine and Sphingosine-1-Phosphate in Arabidopsis

• Published in: Plant physiology (Bethesda) Vol. 149; no. 1; pp. 487 - 498
• Main Authors: Michaelson, Louise V., Zäuner, Simone, Markham, Jonathan E., Haslam, Richard P., Desikan, Radhika, Mugford, Sarah, Albrecht, Sandra, Warnecke, Dirk, Sperling, Petra, Heinz, E., Napier, Johnathan A.
• Format: Journal Article
• Language: English
• Published: American Society of Plant Biologists 2009
• Subjects: Alleles; Biochemical Processes and Macromolecular Structures; Fatty acids; Genes; Guard cells; Lipids; Open reading frames; Plants; Pollen; Polymerase chain reaction; Sphingolipids

The role of Δ4-unsaturated sphingolipid long-chain bases such as sphingosine was investigated in Arabidopsis (Arabidopsis thaliana). Identification and functional characterization of the sole Arabidopsis ortholog of the sphingolipid Δ4-desaturase was achieved by heterologous expression in Pichia pas...