Genome-Wide Analysis of Cotton MYB Transcription Factors and the Functional Validation of GhMYB in Response to Drought Stress

• Published in: Plant and cell physiology Vol. 65; no. 1; pp. 79 - 94
• Main Authors: Su, Jiuchang, Zhan, Na, Cheng, Xiaoru, Song, Shanglin, Dong, Tianyu, Ge, Xiaoyang, Duan, Hongying
• Format: Journal Article
• Language: English
• Published: UK Oxford University Press 19.01.2024
• Subjects: Arabidopsis - genetics; Arabidopsis - metabolism; Droughts; Gene Expression Regulation, Plant; Genes, myb; Gossypium - genetics; Gossypium - metabolism; Multigene Family; Phylogeny; Plant Proteins - genetics; Plant Proteins - metabolism; Stress, Physiological - genetics; Transcription Factors - genetics; Transcription Factors - metabolism; Drought stress; Cotton; transcription factors; Functional validation; Genome-wide analysis; MYB transcription factors
• DOI: 10.1093/pcp/pcad125
• ISSN: 0032-0781

Abstract MYB transcription factors play important roles during abiotic stress responses in plants. However, little is known about the accurate systematic analysis of MYB genes in the four cotton species, Gossypium hirsutum, G. barbadense, G. arboreum and G. raimondii. Herein, we performed phylogenetic analysis and showed that cotton MYBs and Arabidopsis MYBs were clustered in the same subfamilies for each species. The identified cotton MYBs were distributed unevenly on chromosomes in various densities for each species, wherein genome-wide tandem and segment duplications were the main driving force of MYB family expansion. Synteny analysis suggested that the abundant collinearity pairs of MYBs were identified between G. hirsutum and the other three species, and that they might have undergone strong purification selection. Characteristics of conserved motifs, along with their consensus sequence, promoter cis elements and gene structure, revealed that MYB proteins might be highly conserved in the same subgroups for each species. Subsequent analysis of differentially expressed genes and expression patterns indicated that most GhMYBs might be involved in response to drought (especially) and salt stress, which was supported by the expression levels of nine GhMYBs using real-time quantitative PCR. Finally, we performed a workflow that combined virus-induced gene silencing and the heterologous transformation of Arabidopsis, which confirmed the positive roles of GhMYBs under drought conditions, as validated by determining the drought-tolerant phenotypes, damage index and/or water loss rate. Collectively, our findings not only expand our understanding of the relationships between evolution and function of MYB genes, but they also provide candidate genes for cotton breeding.