Chloroplast-diphenyl ether interactions. II

• Published in: Plant physiology (Bethesda) Vol. 78; no. 2; pp. 215 - 220
• Main Authors: WETTLAUFER, S. H, ALSCHER, R, STRICK, C
• Format: Journal Article
• Language: English
• Published: Rockville, MD American Society of Plant Physiologists 01.06.1985
• Subjects: Biological and medical sciences; Chemical control; Chloroplasts; Enzymes; Ethers; fisiologia; Fundamental and applied biological sciences. Psychology; Herbicides; Oxygen; Parasitic plants. Weeds; Phosphates; Photosynthesis; physiologie; physiology; Phytopathology. Animal pests. Plant and forest protection; Plants; plaste; plastidos; plastids; Spinach; spinacia oleracea; Thylakoids; Weeds; Vegetable crop; Dicotyledones; Angiospermae; Pesticides; Spermatophyta; Spinacia oleracea; Mechanism of action; Photosynthesis; Greenhouse study; Chloroplast; Herbicide; Chenopodiaceae
• DOI: 10.1104/pp.78.2.215
• ISSN: 0032-0889

Acifluorfen, a p-nitrodiphenyl ether herbicide, is inhibitory to those photosynthetic functions that require a functioning chloroplast envelope. Functions involving the stroma are also affected. Acifluorfen does not lyse intact spinach chloroplasts, yet does increase the sensitivity of CO2-dependent O2 evolution to exogenous inorganic phosphate without directly affecting the function of the phosphate translocator. Acifluorfen penetrates into the chloroplast stroma in a light-independent fashion. Once inside, it causes the inactivation of light and dithiothreitol-activated fructose 1,6-bisphosphatase. Light-activated glyceraldehyde-3-phosphate dehydrogenase (NADP) is also inactivated by acifluorfen. These data suggest that acifluorfen stimulates a pathway for inactivation of fructose 1,6-bisphosphatase and glyceraldehyde 3-phosphate dehydrogenase (NADP) which uses oxygen as a terminal oxidant and which involves thioredoxin and ferredoxin-thioredoxin reductase.