Evidence for direct action of testosterone on rat liver cells : in vivo and in vitro induction of unusual estrogen-binding protein
We demonstrate that on the rat liver, testosterone (T) induced differentiated functions and enhanced unusual estrogen-binding protein (UEBP) content through mechanisms dependent on cell activation by androgens, the presence of growth hormone (GH) and the hormonal status of the animal. To determine w...
Saved in:
Published in | The Journal of steroid biochemistry and molecular biology Vol. 42; no. 2; pp. 243 - 249 |
---|---|
Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Oxford
Elsevier Science
01.04.1992
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | We demonstrate that on the rat liver, testosterone (T) induced differentiated functions and enhanced unusual estrogen-binding protein (UEBP) content through mechanisms dependent on cell activation by androgens, the presence of growth hormone (GH) and the hormonal status of the animal. To determine whether liver cells are a target for androgens, we measured T effects on UEBP in gonadectomized adult male and female rats in vivo and in vitro. In ovariectomized rats, T increased 8- to 9-fold UEBP levels that remained constant during 10 days. Also in vitro, using hepatocytes from ovariectomized rats, T alone increased UEBP levels 3-fold in a dose-response pattern. Combining a fixed low dose of GH with different concentrations of T increased UEBP 2-fold above T alone. Whereas GH alone had no effects in ovariectomized rats, hepatocytes were responsive to GH, in a dose dependent pattern that was abolished when T was used together with GH. On the other hand, T alone had no effect in hypophysectomized-ovariectomized animals. The latter group was rendered T responsive after the simultaneous injection of GH with T that increased UEBP content 6.6-fold in vivo. Castrated males revealed a marked responsiveness to T and GH in vivo and in vitro, when added separately or in combination. The results obtained suggest a complex regulatory system and we conclude that T acts directly on rat liver as: (1) an inducer of sex differentiation; and (2) a regulator of UEBP production in males. In addition, liver regeneration studies in castrated-hypophysectomized males revealed the UEBP phenotype in daughter cells in the absence of treatment. |
---|---|
AbstractList | We demonstrate that on the rat liver, testosterone (T) induced differentiated functions and enhanced unusual estrogen-binding protein (UEBP) content through mechanisms dependent on cell activation by androgens, the presence of growth hormone (GH) and the hormonal status of the animal. To determine whether liver cells are a target for androgens, we measured T effects on UEBP in gonadectomized adult male and female rats in vivo and in vitro. In ovariectomized rats, T increased 8- to 9-fold UEBP levels that remained constant during 10 days. Also in vitro, using hepatocytes from ovariectomized rats, T alone increased UEBP levels 3-fold in a dose-response pattern. Combining a fixed low dose of GH with different concentrations of T increased UEBP 2-fold above T alone. Whereas GH alone had no effects in ovariectomized rats, hepatocytes were responsive to GH, in a dose dependent pattern that was abolished when T was used together with GH. On the other hand, T alone had no effect in hypophysectomized-ovariectomized animals. The latter group was rendered T responsive after the simultaneous injection of GH with T that increased UEBP content 6.6-fold in vivo. Castrated males revealed a marked responsiveness to T and GH in vivo and in vitro, when added separately or in combination. The results obtained suggest a complex regulatory system and we conclude that T acts directly on rat liver as: (1) an inducer of sex differentiation; and (2) a regulator of UEBP production in males. In addition, liver regeneration studies in castrated-hypophysectomized males revealed the UEBP phenotype in daughter cells in the absence of treatment. We demonstrate that on the rat liver, testosterone (T) induced differentiated functions and enhanced unusual estrogen-binding protein (UEBP) content through mechanisms dependent on cell activation by androgens, the presence of growth hormone (GH) and the hormonal status of the animal. The results obtained suggest a complex regulatory system and we conclude that T acts directly on rat liver as: (1) an inducer of sex differentiation; and (2) a regulator of UEBP production in males. In addition, liver regeneration studies in castrated-hypophysectomized males revealed the UEBP phenotype in daughter cells in the absence of treatment. These findings provide novel insights in eukaryotic systems where a T-imprinted phenotype continued to be expressed after withdrawal of hormonal determinants. |
Author | SMIRNOVA, O. V BOCHAROV, A. V KOVTUN, I. V VISHNYAKOVA, T. G ROZEN, V. B |
Author_xml | – sequence: 1 givenname: O. V surname: SMIRNOVA fullname: SMIRNOVA, O. V organization: M.V. Lomonosov Moscow state univ., school biology, lab. endocrinology, 119899 Moscow, Ussr – sequence: 2 givenname: T. G surname: VISHNYAKOVA fullname: VISHNYAKOVA, T. G organization: M.V. Lomonosov Moscow state univ., school biology, lab. endocrinology, 119899 Moscow, Ussr – sequence: 3 givenname: A. V surname: BOCHAROV fullname: BOCHAROV, A. V organization: M.V. Lomonosov Moscow state univ., school biology, lab. endocrinology, 119899 Moscow, Ussr – sequence: 4 givenname: I. V surname: KOVTUN fullname: KOVTUN, I. V organization: M.V. Lomonosov Moscow state univ., school biology, lab. endocrinology, 119899 Moscow, Ussr – sequence: 5 givenname: V. B surname: ROZEN fullname: ROZEN, V. B organization: M.V. Lomonosov Moscow state univ., school biology, lab. endocrinology, 119899 Moscow, Ussr |
BackLink | http://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5351155$$DView record in Pascal Francis https://www.ncbi.nlm.nih.gov/pubmed/1567787$$D View this record in MEDLINE/PubMed |
BookMark | eNqFkU-LFDEQxYOsrLOr30AhBxE9tFaS6aSzN1l2VFjwoueQv0ukJ1mT9IBXP7lpZxiP5pKi3q9eJbwrdJFy8gi9JPCeAOEfQHIYQHB4K-k7CcDYsHuCNmQSciCUwgXanJFn6KrWH7BCRFyiSzJyISaxQb_vDtH5ZD0OuWAXi7cNa9tiTjgH3HxtuTZf-mrcW0U3PMeDL9j6ea74BseED_GQsU7uWLeSe-GWs8eSlrroGXerkh98GkyXY3rAjyU3H9Nz9DToufoXp_safd_dfbv9PNx__fTl9uP9YKkQbfBMeklccJSYKYAllHNj2SRECNJID-DMNkBvT0YaZwQEFuzEje7HbCW7Rm-Ovn3vz6W_Ru1jXb-hk89LVYJK4MD4f0HCR8omIjq4PYK25FqLD-qxxL0uvxQBtWak1gDUGoCSVP3NSO362KuT_2L23v0bOobS9dcnXVer51B0srGesZGNhIwj-wM-R53I |
CitedBy_id | crossref_primary_10_1016_j_biomed_2012_09_001 crossref_primary_10_1016_0303_7207_94_90037_X crossref_primary_10_1134_S1062360409050026 crossref_primary_10_1007_BF02935682 crossref_primary_10_1016_0960_0760_93_90023_P crossref_primary_10_1007_BF02446019 crossref_primary_10_1016_j_transproceed_2005_10_123 |
Cites_doi | 10.1016/0022-4731(90)90254-P 10.1677/joe.0.1110205 10.1016/S0021-9258(19)52451-6 10.1210/endo-128-1-349 10.1677/joe.0.1100123 10.1016/0022-4731(85)90193-1 10.1210/endo-122-3-991 10.1210/endo-120-4-1551 10.1146/annurev.ph.45.030183.000411 10.1016/0003-9861(89)90358-5 10.1042/bj0620315 10.1146/annurev.ph.45.030183.000345 10.1016/0022-4731(83)90237-6 10.1016/S0074-7696(08)60583-5 10.1016/0022-4731(83)90058-4 |
ContentType | Journal Article |
Copyright | 1992 INIST-CNRS |
Copyright_xml | – notice: 1992 INIST-CNRS |
DBID | IQODW CGR CUY CVF ECM EIF NPM AAYXX CITATION 7TK 7X8 |
DOI | 10.1016/0960-0760(92)90033-F |
DatabaseName | Pascal-Francis Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed CrossRef Neurosciences Abstracts MEDLINE - Academic |
DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) CrossRef Neurosciences Abstracts MEDLINE - Academic |
DatabaseTitleList | MEDLINE Neurosciences Abstracts |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Chemistry |
EISSN | 1879-1220 |
EndPage | 249 |
ExternalDocumentID | 10_1016_0960_0760_92_90033_F 1567787 5351155 |
Genre | Journal Article |
GroupedDBID | --- --K --M -~X .55 .GJ .~1 08R 0R~ 123 1B1 1RT 1~. 1~5 29L 3O- 4.4 457 4G. 53G 5VS 7-5 71M 8P~ 9JM AACTN AAEDT AAEDW AAIAV AAIKJ AAKOC AALRI AAOAW AAQFI AAQXK AAUGY AAXUO ABFNM ABFRF ABGSF ABMAC ABPIF ABPTK ABUDA ABXDB ABYKQ ACDAQ ACGFS ACIUM ACPRK ACRLP ADBBV ADEZE ADMUD ADUVX AEBSH AEFWE AEHWI AEKER AENEX AFKWA AFTJW AFXIZ AGHFR AGRDE AGUBO AGYEJ AHHHB AIEXJ AIKHN AITUG AJBFU AJOXV ALMA_UNASSIGNED_HOLDINGS AMFUW AMRAJ ASPBG AVWKF AXJTR AZFZN BKOJK BLXMC CS3 DOVZS DU5 EBS EFJIC EJD EO8 EO9 EP2 EP3 F5P FDB FEDTE FGOYB FIRID FNPLU FYGXN G-2 G-Q GBLVA HLW HZ~ IH2 IHE IQODW J1W KOM L7B LX3 M41 MO0 N9A O-L O9- OAUVE OZT P-8 P-9 P2P PC. Q38 R2- RIG ROL RPZ SBG SDF SDG SDP SES SEW SPCBC SSU SSZ T5K WUQ X7M ZGI ZXP ~G- AAXKI ABJNI AFJKZ AKRWK CGR CUY CVF ECM EIF HVGLF NPM AAYXX CITATION 7TK 7X8 |
ID | FETCH-LOGICAL-c277t-e39e91dfd21b8f0c1266bc3877ff9b9e00db4f01268b9bdb70f3fc86baaaab493 |
ISSN | 0960-0760 |
IngestDate | Fri Aug 16 21:05:27 EDT 2024 Fri Aug 16 23:35:46 EDT 2024 Thu Sep 26 18:47:25 EDT 2024 Sat Sep 28 08:39:45 EDT 2024 Sun Oct 29 17:08:44 EDT 2023 |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 2 |
Keywords | Androgen Rat Liver STH Rodentia Estrogen Binding protein Testosterone Vertebrata Mammalia Adenohypophyseal hormone Hepatocyte Sexual dimorphism Sex steroid hormone |
Language | English |
License | CC BY 4.0 |
LinkModel | OpenURL |
MergedId | FETCHMERGED-LOGICAL-c277t-e39e91dfd21b8f0c1266bc3877ff9b9e00db4f01268b9bdb70f3fc86baaaab493 |
Notes | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
PMID | 1567787 |
PQID | 16523817 |
PQPubID | 23462 |
PageCount | 7 |
ParticipantIDs | proquest_miscellaneous_72906036 proquest_miscellaneous_16523817 crossref_primary_10_1016_0960_0760_92_90033_F pubmed_primary_1567787 pascalfrancis_primary_5351155 |
PublicationCentury | 1900 |
PublicationDate | 1992-Apr |
PublicationDateYYYYMMDD | 1992-04-01 |
PublicationDate_xml | – month: 04 year: 1992 text: 1992-Apr |
PublicationDecade | 1990 |
PublicationPlace | Oxford |
PublicationPlace_xml | – name: Oxford – name: England |
PublicationTitle | The Journal of steroid biochemistry and molecular biology |
PublicationTitleAlternate | J Steroid Biochem Mol Biol |
PublicationYear | 1992 |
Publisher | Elsevier Science |
Publisher_xml | – name: Elsevier Science |
References | Gustafasson (10.1016/0960-0760(92)90033-F_BIB1) 1983; 45 Jeffery (10.1016/0960-0760(92)90033-F_BIB7) 1986; 110 Gleiberman (10.1016/0960-0760(92)90033-F_BIB19) 1985; 95 Miroshnichenko (10.1016/0960-0760(92)90033-F_BIB13) 1983; 18 El'chanininova (10.1016/0960-0760(92)90033-F_BIB8) 1988; 53 Smirnova (10.1016/0960-0760(92)90033-F_BIB5) 1985; 23 Jansson (10.1016/0960-0760(92)90033-F_BIB4) 1987; 120 Smirnova (10.1016/0960-0760(92)90033-F_BIB6) 1986; 32 Smirnov (10.1016/0960-0760(92)90033-F_BIB12) 1988; 34 Cammisa (10.1016/0960-0760(92)90033-F_BIB11) 1988; 122 Smirnova (10.1016/0960-0760(92)90033-F_BIB14) 1990; 35 Smirnova (10.1016/0960-0760(92)90033-F_BIB9) 1988; 34 Lowry (10.1016/0960-0760(92)90033-F_BIB15) 1951; 193 Vind (10.1016/0960-0760(92)90033-F_BIB17) 1990; 275 Song (10.1016/0960-0760(92)90033-F_BIB21) 1991; 128 Sarkar (10.1016/0960-0760(92)90033-F_BIB20) 1986; 111 Oleinik (10.1016/0960-0760(92)90033-F_BIB10) 1990; 36 Rozen (10.1016/0960-0760(92)90033-F_BIB18) 1990 Gustafsson (10.1016/0960-0760(92)90033-F_BIB3) 1983; 120 Burton (10.1016/0960-0760(92)90033-F_BIB16) 1956; 62 Roy (10.1016/0960-0760(92)90033-F_BIB2) 1983; 45 |
References_xml | – volume: 35 start-page: 457 year: 1990 ident: 10.1016/0960-0760(92)90033-F_BIB14 article-title: In vivo and in vitro estimation of the direct effect of estrogen on rat hepatocytes tested by the changes in the unusual estrogen-binding protein content publication-title: J. Steroid Biochem. doi: 10.1016/0022-4731(90)90254-P contributor: fullname: Smirnova – volume: 111 start-page: 205 year: 1986 ident: 10.1016/0960-0760(92)90033-F_BIB20 article-title: Cellular interaction in the hormonal induction of α2μ-globulin in rat liver publication-title: Endocrinology doi: 10.1677/joe.0.1110205 contributor: fullname: Sarkar – volume: 193 start-page: 1 year: 1951 ident: 10.1016/0960-0760(92)90033-F_BIB15 article-title: Protein measurement with the Folin phenol reagent publication-title: J. Biol. Chem. doi: 10.1016/S0021-9258(19)52451-6 contributor: fullname: Lowry – volume: 36 start-page: 61 year: 1990 ident: 10.1016/0960-0760(92)90033-F_BIB10 article-title: Specificities of sex-differentiating action of sex steroids on androgen receptors in rat liver publication-title: Problemy Endocr. (U.S.S.R.) contributor: fullname: Oleinik – volume: 128 start-page: 349 year: 1991 ident: 10.1016/0960-0760(92)90033-F_BIB21 article-title: Androgen receptor messenger ribonuclein acid (mRNA) in the rat liver publication-title: Endocrinology doi: 10.1210/endo-128-1-349 contributor: fullname: Song – volume: 34 start-page: 68 year: 1988 ident: 10.1016/0960-0760(92)90033-F_BIB9 article-title: Determination of sex differentation of the level of androgen receptors in rat liver cytosol publication-title: Problemy Endocr. (U.S.S.R.) contributor: fullname: Smirnova – volume: 110 start-page: 123 year: 1986 ident: 10.1016/0960-0760(92)90033-F_BIB7 article-title: Effect of hypophysectomy and growth hormone infusion on rat hepatic carbonic anhydrases publication-title: J. Endocr. doi: 10.1677/joe.0.1100123 contributor: fullname: Jeffery – volume: 34 start-page: 74 year: 1988 ident: 10.1016/0960-0760(92)90033-F_BIB12 article-title: Regulation of steroid hormone dynamics by steroid interaction with intracellular steromoduline proteins publication-title: Problemy Endocr. (U.S.S.R.) contributor: fullname: Smirnov – volume: 23 start-page: 461 year: 1985 ident: 10.1016/0960-0760(92)90033-F_BIB5 article-title: The unusual estrogen-binding protein (UEBP) of rat liver: the role of sex steroids and hypophysis in its regulation publication-title: J. Steroid Biochem. doi: 10.1016/0022-4731(85)90193-1 contributor: fullname: Smirnova – volume: 122 start-page: 991 year: 1988 ident: 10.1016/0960-0760(92)90033-F_BIB11 article-title: Hormonal regulation of pseudocholineesterase activity in cultured rat hepatocytes publication-title: Endocrinology doi: 10.1210/endo-122-3-991 contributor: fullname: Cammisa – volume: 120 start-page: 1551 year: 1987 ident: 10.1016/0960-0760(92)90033-F_BIB4 article-title: Differential effects of neonatal and adult androgen exposure on the growth hormone secretory pattern in male rats publication-title: Endocrinology doi: 10.1210/endo-120-4-1551 contributor: fullname: Jansson – volume: 32 start-page: 65 year: 1986 ident: 10.1016/0960-0760(92)90033-F_BIB6 article-title: A study of some mechanisms of the hypophysis and somatotropin in the hormonal regulation of the level of unusual estrogen-binding protein in rat liver publication-title: Problem Endocr. (U.S.S.R.) contributor: fullname: Smirnova – volume: 45 start-page: 51 year: 1983 ident: 10.1016/0960-0760(92)90033-F_BIB1 article-title: Sex steroid induced changes in hepatic enzymes publication-title: A. Rev. Physiol. doi: 10.1146/annurev.ph.45.030183.000411 contributor: fullname: Gustafasson – volume: 53 start-page: 452 year: 1988 ident: 10.1016/0960-0760(92)90033-F_BIB8 article-title: Molecular and hormone-binding properties of a partially purified preparation of the androgen receptor from the livers of male rats publication-title: Biokhimiya (U.S.S.R.) contributor: fullname: El'chanininova – volume: 275 start-page: 140 year: 1990 ident: 10.1016/0960-0760(92)90033-F_BIB17 article-title: Effect of cytochrome P-450 inducing agents on the monooxygenation of testosterone in long-term cultures of hepatocytes from male and female rats publication-title: Archs Biochem. Biophys. doi: 10.1016/0003-9861(89)90358-5 contributor: fullname: Vind – volume: 62 start-page: 315 year: 1956 ident: 10.1016/0960-0760(92)90033-F_BIB16 article-title: A study of the conditions and the mechanisms of the diphenylamine reaction for the colorimetric estimation of deoxyribomicleic acid publication-title: Biochem. J. doi: 10.1042/bj0620315 contributor: fullname: Burton – volume: 45 start-page: 37 year: 1983 ident: 10.1016/0960-0760(92)90033-F_BIB2 article-title: Sexual dimorphism in the liver publication-title: A. Rev. Physiol. doi: 10.1146/annurev.ph.45.030183.000345 contributor: fullname: Roy – volume: 120 start-page: 691 year: 1983 ident: 10.1016/0960-0760(92)90033-F_BIB3 article-title: Regulation of sexual dimorphism hepatic steroid metabolism by the somatostatin-growth hormone axis publication-title: J. Steroid Biochem. doi: 10.1016/0022-4731(83)90237-6 contributor: fullname: Gustafsson – start-page: 156 year: 1990 ident: 10.1016/0960-0760(92)90033-F_BIB18 article-title: The main principles and hormonal mechanisms of sex differentiation of liver functions publication-title: Suzdal (U.S.S.R.) contributor: fullname: Rozen – volume: 95 start-page: 229 year: 1985 ident: 10.1016/0960-0760(92)90033-F_BIB19 article-title: Cell position and cell interactions in expression of fetal phenotype of hepatocyte publication-title: Intern. Rev. Cytol. doi: 10.1016/S0074-7696(08)60583-5 contributor: fullname: Gleiberman – volume: 18 start-page: 403 year: 1983 ident: 10.1016/0960-0760(92)90033-F_BIB13 article-title: The unusual estrogen-binding protein (UEBP) of male rat liver: structural determinants of ligands publication-title: J. Steroid Biochem. doi: 10.1016/0022-4731(83)90058-4 contributor: fullname: Miroshnichenko |
SSID | ssj0003317 |
Score | 1.4518292 |
Snippet | We demonstrate that on the rat liver, testosterone (T) induced differentiated functions and enhanced unusual estrogen-binding protein (UEBP) content through... |
SourceID | proquest crossref pubmed pascalfrancis |
SourceType | Aggregation Database Index Database |
StartPage | 243 |
SubjectTerms | Animals Biological and medical sciences Carrier Proteins - biosynthesis Female Fundamental and applied biological sciences. Psychology Hypophysectomy Liver - cytology Liver - metabolism Liver - physiology Liver Regeneration Liver. Bile. Biliary tracts Male Orchiectomy Ovariectomy Rats Receptors, Estrogen Testosterone - physiology Vertebrates: digestive system |
Title | Evidence for direct action of testosterone on rat liver cells : in vivo and in vitro induction of unusual estrogen-binding protein |
URI | https://www.ncbi.nlm.nih.gov/pubmed/1567787 https://search.proquest.com/docview/16523817 https://search.proquest.com/docview/72906036 |
Volume | 42 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1bb9MwFLZK9wASQtwmCgz8ABKoSpe7E97GRNmoAAl1Y29RHNuiApKpTSrBG3-Q38Q5tpNmgyFGHyLrpDm28n2Jj51zIeRJLLwgiQPmKMaEExaedHjupk6iIukFUrKc40Lx7bv44Ch8cxKdDAY_e15LTc0nxfc_xpX8D6ogA1wxSvYSyHZKQQBtwBeOgDAc_wnjtiSo9hU0k9PY1v7GT_9YNAbzIFRgSIIIsB5_QTeMMe7Wa1e4w3K8Xqwrm4IJ2vWygoZoOiVN2awwwgR0LSsYCK6khYlgr7BQZt-43YSZaQNXd70AA3eBVblMWTnd09e2JO_YpoDqbfMssUgr4v5-cjxp5ceL1afyW_7ZnppPXnenXlYYN1at9Tuud8msWteNiWprpcIG-_k9lxi7SRm7Dn477L-vQ7_HS7__8jUJn-w87ptUqL9NEWa3otMMdnzqP8VJGavaTTfTYusKcG627HwYW_c41JShpiz1M60lm14hWz5Lo2hItvZmHz7OOtsgCHQN6K73NpjTi3c72bPUf25Hc8ZYun6ar-C5VabgysUrIm0ZzW-SGxZxumf4eYsMZHmbXN1vIb9DfrQ8pcBTanhKDU9ppWifpxREwFOqeUo1T1_Qw5IiSylwhy6wDVSkHUtRhWUpPc9Sall6lxxNX833Dxxb-sMpfMZqRwapTD2hhO_xRLmFB3YkL4KEMaVSnkrXFTxUYFzFCU-54MxVgSqSmOfw42EabJNhCaO-Ryj3itD1RFhEeRAqJbgoEoYL6yiXDMzZEXHae5ydmgwv2d-wHZGdM0B0F0X4TT6KRuRxC0wG9xnvVF7KqlllXhyhgcwu_gfD4gtgU47ItkF0M6IIMz2y-5cc7ANybfNcPSTDetnIHbCia_7IUvMXYrbJAA |
link.rule.ids | 315,786,790,27955,27956 |
linkProvider | Library Specific Holdings |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Evidence+for+direct+action+of+testosterone+on+rat+liver+cells%3A+In+vivo+and+in+vitro+induction+of+unusual+estrogen-binding+protein&rft.jtitle=The+Journal+of+steroid+biochemistry+and+molecular+biology&rft.au=Smirnova%2C+O.V.&rft.au=Vishnyakova%2C+T.G.&rft.au=Bocharov%2C+A.V.&rft.au=Kovtun%2C+I.V.&rft.date=1992-04-01&rft.issn=0960-0760&rft.volume=42&rft.issue=2&rft.spage=243&rft.epage=249&rft_id=info:doi/10.1016%2F0960-0760%2892%2990033-F&rft.externalDBID=n%2Fa&rft.externalDocID=10_1016_0960_0760_92_90033_F |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0960-0760&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0960-0760&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0960-0760&client=summon |