Analysis of Transcriptional Profiles to Discover Biomarker Candidates in Mycobacterium avium subsp. paratuberculosis-Infected Macrophages, RAW 264.7

• Published in: Journal of microbiology and biotechnology Vol. 23; no. 8; pp. 1167 - 1175
• Main Authors: Cha, S.B., Seoul National University, Seoul, Republic of korea, Yoo, A., Seoul National University, Seoul, Republic of korea, Park, H.T., Seoul National University, Seoul, Republic of korea, Sung, K.Y., Seoul National University, Seoul, Republic of korea, Shin, M.K., Seoul National University, Seoul, Republic of korea, Yoo, H.S., Seoul National University, Seoul, Republic of korea
• Format: Journal Article
• Language: English
• Published: Seoul Korean Society for Applied Microbiology 01.08.2013; 한국미생물·생명공학회
• Subjects: Animals; Biological and medical sciences; biomarker,transcriptional change; Biomarkers; Biotechnology; Cell Line; Fundamental and applied biological sciences. Psychology; Gene Expression Profiling; Host-Pathogen Interactions; Macrophages - microbiology; Mice; MYCOBACTERIUM AVIUM; Mycobacterium avium subsp. paratuberculosis - growth & development; Real-Time Polymerase Chain Reaction; Time Factors; 생물학; transcriptional change; Disease; Transcription; Paratuberculosis; Biological marker; Mycobacterium avium; Mycobacterial infection; biomarker; Infection; Mycobacteriales; Johne's disease; Bacteriosis; Mycobacteriaceae; Bacteria; Actinomycetes; Macrophage
• DOI: 10.4014/jmb.1302.02021
• ISSN: 1017-7825

Paratuberculosis (PTB) or Johne’s disease is one of the most serious chronic debilitating diseases of ruminants worldwide that is caused by Mycobacterium avium subsp. paratuberculosis (MAP). MAP is a slow-growing bacterium that has very long latent periods, resulting in difficulties in diagnosing and controlling the disease, especially regarding the diagnosis of fecal shedders of MAP without any clinical signs. Based on this situation, attempts were made to identify biomarkers that show early responses to MAP infection in a macrophage cell line, RAW 264.7. In response to the infection with the bacterium, a lot of genes were turned on and/or off in the cells. Of the altered genes, three different categories were identified based on the time-dependent gene expression patterns. Those genes were considered as possible candidates for biomarkers of MAP infection after confirmation by quantitative RT-PCR analysis. To the best of our knowledge, this is the first attempt at discovering the host transcriptomic biomarkers of PTB, although further investigation will be required to determine whether these biomarker candidates are associated within the natural host.