Effect of Bmi-1-mediated NF- κ B signaling pathway on the stem-like properties of CD133 + human liver cancer cells

• Published in: Cancer biomarkers : section A of Disease markers Vol. 22; no. 3; pp. 575 - 585
• Main Authors: Ma, De-Qiang, Zhang, Yin-Hua, Ding, De-Ping, Li, Juan, Chen, Lin-Li, Tian, You-You, Ao, Kang-Jian
• Format: Journal Article
• Language: English
• Published: London, England SAGE Publications 01.01.2018; Sage Publications Ltd
• Subjects: Apoptosis; Biological properties; Bmi protein; Cancer; Cell cycle; Cell migration; Colonies; Flow cytometry; Hepatocytes; Immunofluorescence; Liver; Liver cancer; NF-κB protein; Nuclear transport; Oct-4 protein; Programmable logic controllers; Signal transduction; Signaling; siRNA; Stem cells; Translocation; Western blotting; Liver cancer stem cells; Bmi-1; NF-κB pathway; CD133

OBJECTIVE: To investigate the impact of Bmi-1-mediated NF- κ B pathway on the biological characteristics of CD133 + liver cancer stem cells (LCSCs). METHODS: Flow cytometry was used to isolate CD133 + LCSC cells from Huh7, Hep3B, SK-hep1, and PLC/PRF-5 cells. CD133 + Huh7 cells were divided into Con...