Complete sequence and gene organization of the genome of a hyper-thermophilic archaebacterium, Pyrococcus horikoshii OT3

• Published in: DNA research Vol. 5; no. 2; pp. 55 - 76
• Main Authors: Kawarabayasi, Y, Sawada, M, Horikawa, H, Haikawa, Y, Hino, Y, Yamamoto, S, Sekine, M, Baba, S, Kosugi, H, Hosoyama, A, Nagai, Y, Sakai, M, Ogura, K, Otsuka, R, Nakazawa, H, Takamiya, M, Ohfuku, Y, Funahashi, T, Tanaka, T, Kudoh, Y, Yamazaki, J, Kushida, N, Oguchi, A, Aoki, K, Kikuchi, H
• Format: Journal Article
• Language: English
• Published: England 1998
• Subjects: Chromosomes, Archaeal; Codon; DNA, Archaeal - genetics; DNA, Archaeal - isolation & purification; Genes, Archaeal; Genetic Vectors; Genome; Genomic Library; Molecular Sequence Data; Open Reading Frames; Polymerase Chain Reaction; Pyrococcus - genetics; Restriction Mapping; RNA, Archaeal - genetics; RNA, Transfer - genetics; rRNA Operon - genetics; Sequence Analysis, DNA

The complete sequence of the genome of a hyper-thermophilic archaebacterium, Pyrococcus horikoshii OT3, has been determined by assembling the sequences of the physical map-based contigs of fosmid clones and of long polymerase chain reaction (PCR) products which were used for gap-filling. The entire...