LncRNA NEAT1 targets miR‐125/ADAM9 mediated NF‐κB pathway in inflammatory response of rosacea

• Published in: Skin research and technology Vol. 30; no. 7; pp. e13630 - n/a
• Main Authors: Xu, Sijia, Dong, Wenxin
• Format: Journal Article
• Language: English
• Published: Copenhagen John Wiley & Sons, Inc 01.07.2024; John Wiley and Sons Inc
• Subjects: ADAM9; Chromosome 5; Gene expression; Inflammation; inflammatory reaction; Inflammatory response; Interleukin 6; miR‐134‐3p; NEAT1; Original; Phenotypes; Proteins; rose acne; Signal transduction; Skin diseases; TLR2 protein; Toll-like receptors; Western blotting
• ISSN: 0909-752X; 1600-0846; 1600-0846
• DOI: 10.1111/srt.13630

Objective To investigate the role of NEAT1 targeted regulation of miR‐125/ADAM9 mediated NF‐κB pathway in inflammatory response in rosacea. Method HaCaT cell rosacea phenotype was induced by LL37. The connection targeted by NEAT1 and miR‐125a‐5p was confirmed by Double‐Luciferase report analysis. qPCR was employed to assess the levels of expression for NEAT1, miR‐125a‐5p, and ADAM9 genes. The levels of expression for ADAM9/TLR2/NF‐κB P65 pathway proteins in each batch of cells were determined by Western blotting. The levels of expression for inflammatory factors, including TNF‐α, IL‐1β, IL‐6, and IL‐18, were measured through ELISA experimentation. Results LL37 could successfully induce HaCaT cells to exhibit rosacea phenotype. The luciferase report experiment confirmed that NEAT1 could target and bind miR‐125a‐5p and inhibit its expression. ADAM9 exhibited increased expression in LL37‐induced HaCaT cells, showing a positive association with NEAT1 expression and inverse relationship with miR‐125a‐5p activation. LL37 treatment promoted the expression of ADAM9/TLR2/NF‐κB P65 pathway proteins. Silencing ADAM9 can inhibit the inflammatory signaling pathway and reduce the level of TNF‐α, IL‐1β, IL‐6, and IL‐18 in HaCaT cells. Conclusion NEAT1 can suppress the production of miR‐125a‐5p and activate the TLR2/NF‐κB inflammatory pathway mediated by ADAM9, thereby promoting the inflammatory response in rosacea.