The effects of a series of prostaglandins on in vitro models of the allergic response and cellular immunity

• Published in: Prostaglandins Vol. 2; no. 6; pp. 519 - 528
• Main Authors: Lichtenstein, L.M., Gillespie, E., Bourne, H.R., Henney, C.S.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.12.1972
• Subjects: Allergens; Animals; Basophils - drug effects; Buffers; Chromium Isotopes; Cyclic AMP - metabolism; Fluorometry; Histamine H1 Antagonists; Histamine Release; Humans; Hypersensitivity, Delayed; Hypersensitivity, Immediate; Immunity, Cellular - drug effects; Immunoglobulin E - biosynthesis; Leukocytes - immunology; Lymphocytes - immunology; Mast Cells - immunology; Mice; Mice, Inbred C57BL - immunology; Pollen; Prostaglandins - pharmacology; Protein Binding; Tritium
• ISSN: 0090-6980
• DOI: 10.1016/S0090-6980(72)80040-6

We report here on the interaction of prostaglandins with two in vitro models of immunologic responses: the antigen induced, IgE mediated release of histamine from isolated human leukocytes and the cytolysis of DBA/2 mouse mastocytoma cells by sensitized splenic lymphocytes from C57B1 mice. Both reactions were similarly and markedly inhibited by prostaglandins in the order E1=E2>A 2=A 1>B2>Fla=F2 α≅=0. The increase in cyclic AMP levels in the effector cells (mouse lymphocytes and human leukocytes) as a result of treatment with the prostaglandins was also studied: the order of activity in this function was the same as that noted for inhibition of the immunologic responses. These data support the hypothesis that the level of intracellular cyclic AMP regulates the expression of immediate and delayed hypersensitivity.