Single Cycle Selection of CD63-targeting Aptamers Using a Microscale Electrophoretic Filtration Device

• Published in: Bunseki kagaku Vol. 72; no. 3; pp. 111 - 116
• Main Authors: TOBITA, Arisa, TAKAO, Junku, ENDO, Tatsuro, HISAMOTO, Hideaki, SUEYOSHI, Kenji
• Format: Journal Article
• Language: English; Japanese
• Published: Tokyo Japan Science and Technology Agency 01.01.2023
• Subjects: Antibodies; Binding; Electrophoresis; Elution; Filtration; Gene sequencing; Hydrogels; Polyacrylamide; Titration calorimetry

Aptamers, which show properties of specific binding to their target molecules, are expected to become an alternative to antibodies for use as molecular recognition probes instead of antibodies. However, long and laborious selection procedures have hindered their application. To overcome the drawback...