Expression of cell cycle regulator genes in KB, a human squamous cell carcinoma cell line, after irradiation

• Published in: International journal of oncology Vol. 17; no. 5; p. 947
• Main Authors: Uno, M, Otsuki, T, Hiratsuka, J, Yoden, E, Aihara, T, Harada, T, Imajo, Y
• Format: Journal Article
• Language: English
• Published: Greece 01.11.2000
• Subjects: Apoptosis - genetics; Apoptosis - radiation effects; Carcinoma, Squamous Cell - pathology; Cell Cycle - genetics; Cell Cycle - radiation effects; Cell Cycle Proteins - biosynthesis; Cell Cycle Proteins - genetics; Cyclin-Dependent Kinase Inhibitor p16 - biosynthesis; Cyclin-Dependent Kinase Inhibitor p21; Cyclin-Dependent Kinase Inhibitor p27; Cyclin-Dependent Kinases - biosynthesis; Cyclin-Dependent Kinases - genetics; Cyclins - biosynthesis; Cyclins - genetics; DNA Damage; DNA, Neoplasm - genetics; DNA, Neoplasm - radiation effects; Fungal Proteins - biosynthesis; Fungal Proteins - genetics; G2 Phase - radiation effects; GADD45 Proteins; Gene Expression Regulation, Neoplastic - radiation effects; Genes, p16; Genes, p53; Humans; Intracellular Signaling Peptides and Proteins; KB Cells - metabolism; KB Cells - radiation effects; Microtubule-Associated Proteins - biosynthesis; Microtubule-Associated Proteins - genetics; Molecular Motor Proteins; Mouth Neoplasms - pathology; Neoplasm Proteins - biosynthesis; Neoplasm Proteins - genetics; Protein Biosynthesis; Proteins - genetics; S-Phase Kinase-Associated Proteins; Saccharomyces cerevisiae Proteins; Tumor Suppressor Protein p53 - biosynthesis; Tumor Suppressor Proteins

DNA damage induced by irradiation causes overexpression of the p53 gene, and subsequently the upregulation of p53 downstream genes involved in cell cycle modification. Irradiated malignant cells which possess wild-type p53 have been known to undergo G1 arrest due to p21/Cip1/Waf1 upregulation. Other...