Analysis of platelet-derived growth factor receptor alpha expression in adult mouse testis
Background: Platelet-derived growth factor receptor alpha (PDGFRα) is essential for various biological processes, including fetal Leydig cell differentiation. The PDGFRαEGFP mouse model, which expresses an eGFP fusion gene under the native Pdgfrα promoter, serves as a valuable resource for exploring...
Saved in:
| Published in | Journal of animal reproduction & biotechnology (Online) Vol. 39; no. 2; pp. 81 - 87 |
|---|---|
| Main Authors | , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
한국동물생명공학회(구 한국수정란이식학회)
30.06.2024
The Korean Society of Animal Reproduction and Biotechnology 사단법인 한국동물생명공학회 |
| Subjects | |
| Online Access | Get full text |
| ISSN | 2671-4639 2671-4663 |
| DOI | 10.12750/JARB.39.2.81 |
Cover
| Abstract | Background: Platelet-derived growth factor receptor alpha (PDGFRα) is essential for various biological processes, including fetal Leydig cell differentiation. The PDGFRαEGFP mouse model, which expresses an eGFP fusion gene under the native Pdgfrα promoter, serves as a valuable resource for exploring PDGFRα’s expression and function in vivo. This study investigates PDGFRα expression in adult testicular cells using PDGFRαEGFP mouse model. Methods: Genotyping PCR and gel electrophoresis were used to confirm the zygosity of PDGFRαEGFP mice. Histological examination and fluorescence imaging were used to identify PDGFRα expression within testicular tissue. Immunohistochemical analysis assessed the co-expression of PDGFRα with c-Kit, ANO-1, and TASK-1 in testicular cells. Results: Genotyping confirmed the heterozygous status of the mice, which is crucial for studies due to the embryonic lethal phenotype observed in homozygotes. Histological and fluorescence imaging revealed that PDGFRα+ cells were primarily located in the interstitial spaces of the testis, specifically within Leydig cells and peritubular myoid cells (PMCs). Immunohistochemical results showed PDGFRα co-localization with c-Kit and ANO-1 in Leydig cells and a complete co-localization with TASK-1 in both Leydig cells and PMCs. Conclusions: The findings demonstrate specific expression of PDGFRα in Leydig cells and PMCs in adult testicular tissue. The co-expression of PDGFRα with c-Kit, ANO-1, and TASK-1 suggests complex regulatory mechanisms, possibly influencing testicular function and broader physiological processes. |
|---|---|
| AbstractList | Background: Platelet-derived growth factor receptor alpha (PDGFRα) is essential for various biological processes, including fetal Leydig cell differentiation. The PDGFRαEGFP mouse model, which expresses an eGFP fusion gene under the native Pdgfrα promoter, serves as a valuable resource for exploring PDGFRα’s expression and function in vivo. This study investigates PDGFRα expression in adult testicular cells using PDGFRαEGFP mouse model. Methods: Genotyping PCR and gel electrophoresis were used to confirm the zygosity of PDGFRαEGFP mice. Histological examination and fluorescence imaging were used to identify PDGFRα expression within testicular tissue. Immunohistochemical analysis assessed the co-expression of PDGFRα with c-Kit, ANO-1, and TASK-1 in testicular cells. Results: Genotyping confirmed the heterozygous status of the mice, which is crucial for studies due to the embryonic lethal phenotype observed in homozygotes. Histological and fluorescence imaging revealed that PDGFRα+ cells were primarily located in the interstitial spaces of the testis, specifically within Leydig cells and peritubular myoid cells (PMCs). Immunohistochemical results showed PDGFRα co-localization with c-Kit and ANO-1 in Leydig cells and a complete co-localization with TASK-1 in both Leydig cells and PMCs. Conclusions: The findings demonstrate specific expression of PDGFRα in Leydig cells and PMCs in adult testicular tissue. The co-expression of PDGFRα with c-Kit, ANO-1, and TASK-1 suggests complex regulatory mechanisms, possibly influencing testicular function and broader physiological processes. Background: Platelet-derived growth factor receptor alpha (PDGFRα) is essential for various biological processes, including fetal Leydig cell differentiation. The PDGFRαEGFP mouse model, which expresses an eGFP fusion gene under the native Pdgfrα promoter, serves as a valuable resource for exploring PDGFRα’s expression and function in vivo. This study investigates PDGFRα expression in adult testicular cells using PDGFRαEGFP mouse model. Methods: Genotyping PCR and gel electrophoresis were used to confirm the zygosity of PDGFRαEGFP mice. Histological examination and fluorescence imaging were used to identify PDGFRα expression within testicular tissue. Immunohistochemical analysis assessed the co-expression of PDGFRα with c-Kit, ANO-1, and TASK-1 in testicular cells. Results: Genotyping confirmed the heterozygous status of the mice, which is crucial for studies due to the embryonic lethal phenotype observed in homozygotes. Histological and fluorescence imaging revealed that PDGFRα+ cells were primarily located in the interstitial spaces of the testis, specifically within Leydig cells and peritubular myoid cells (PMCs). Immunohistochemical results showed PDGFRα co-localization with c-Kit and ANO-1 in Leydig cells and a complete co-localization with TASK-1 in both Leydig cells and PMCs. Conclusions: The findings demonstrate specific expression of PDGFRα in Leydig cells and PMCs in adult testicular tissue. The co-expression of PDGFRα with c-Kit, ANO-1, and TASK-1 suggests complex regulatory mechanisms, possibly influencing testicular function and broader physiological processes. KCI Citation Count: 1 |
| Author | Kang, Dawon Kim, Eun-Jin Woo, Min Seok Ko, Eun-A Lee, Chung Eun Lee, Dong Kun |
| Author_xml | – sequence: 1 givenname: Min Seok orcidid: 0000-0002-0357-9666 surname: Woo fullname: Woo, Min Seok organization: Department of Physiology, College of Medicine and Institute of Medical Sciences, Gyeongsang National University, Jinju 52727, Korea – sequence: 2 givenname: Eun-Jin orcidid: 0000-0003-1068-9334 surname: Kim fullname: Kim, Eun-Jin organization: Department of Physiology, College of Medicine and Institute of Medical Sciences, Gyeongsang National University, Jinju 52727, Korea – sequence: 3 givenname: Dong Kun orcidid: 0000-0001-9969-0117 surname: Lee fullname: Lee, Dong Kun organization: Department of Physiology, College of Medicine and Institute of Medical Sciences, Gyeongsang National University, Jinju 52727, Korea, Department of Convergence Medical Science, Gyeongsang National University, Jinju 52727, Korea – sequence: 4 givenname: Chung Eun orcidid: 0000-0003-4469-7201 surname: Lee fullname: Lee, Chung Eun organization: Department of Thoracic and Cardiovascular Surgery, College of Medicine, Gyeongsang National University Hospital, Gyeongsang National University, Jinju 52727, Korea – sequence: 5 givenname: Eun-A orcidid: 0000-0002-1585-6886 surname: Ko fullname: Ko, Eun-A organization: Department of Physiology, Colledge of Medicine, Jeju National University, Jeju 63243, Korea – sequence: 6 givenname: Dawon orcidid: 0000-0001-7402-7298 surname: Kang fullname: Kang, Dawon organization: Department of Physiology, College of Medicine and Institute of Medical Sciences, Gyeongsang National University, Jinju 52727, Korea, Department of Convergence Medical Science, Gyeongsang National University, Jinju 52727, Korea |
| BackLink | https://www.kci.go.kr/kciportal/ci/sereArticleSearch/ciSereArtiView.kci?sereArticleSearchBean.artiId=ART003095177$$DAccess content in National Research Foundation of Korea (NRF) |
| BookMark | eNp1kc1r3DAQxUVJoek2x94FvRW81aclH7ehTbYECiG99CLG8nhXWccykjZN_vs4uyWHQE4zPH7zZpj3kZyMcURCPnO25MJo9u3X6vr7UjZLsbT8HTkVteGVqmt58tLL5gM5yzm0TGtjrbL8lPxdjTA85pBp7Ok0QMEBS9VhCvfY0U2K_8qW9uBLTDShx-m5gWHaAsWHKeFsF0caRgrdfij0Lu4z0oK5hPyJvO9hyHj2vy7In58_bs4vq6vfF-vz1VXlhZG8EjXK3gvVIcxnKUTV1aoRCF4byTjXLTBZa21bbqTuoRW-gcYCF7aVpm7lgnw9-o6pdzsfXIRwqJvodsmtrm_WjjMtjTF2htdHuItw66YU7iA9HiYOQkwbB6kEP6Dre2Fa1apGNlJJwSwztVbWstZw0LO4IF-OXruYELLfxgGSu437NP80OyUNM2amqiPlU8w5Yf-ylTN3iM49R-dk44SzfOblK96HAmV-c0kQhjemngB9R51M |
| CitedBy_id | crossref_primary_10_4196_kjpp_24_309 |
| Cites_doi | 10.1111/j.1447-0578.2006.00118.x 10.1101/gad.1052503 10.1016/j.mce.2021.111179 10.1242/dev.00721 10.1016/j.bbrc.2022.03.149 10.12750/JARB.37.2.87 10.1371/journal.pone.0182265 10.1101/2023.07.21.549565 10.1016/S1043-2760(01)00518-5 10.1111/j.1582-4934.2011.01510.x 10.12750/JARB.34.4.311 10.1016/j.diff.2022.02.006 10.1016/j.reprotox.2023.108497 10.1002/mrd.23574 10.1083/jcb.131.4.1105 10.12750/JARB.38.4.291 10.7554/eLife.36865 10.1083/jcb.149.5.1019 |
| ContentType | Journal Article |
| DBID | AAYXX CITATION KROLR DOA ACYCR |
| DOI | 10.12750/JARB.39.2.81 |
| DatabaseName | CrossRef Korea Scholar DOAJ Directory of Open Access Journals Korean Citation Index |
| DatabaseTitle | CrossRef |
| DatabaseTitleList | |
| Database_xml | – sequence: 1 dbid: DOA name: DOAJ Directory of Open Access Journals url: https://www.doaj.org/ sourceTypes: Open Website |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Agriculture |
| EISSN | 2671-4663 |
| EndPage | 87 |
| ExternalDocumentID | oai_kci_go_kr_ARTI_10537778 oai_doaj_org_article_ff27b4b493934320807654880b71a539 437077 10_12750_JARB_39_2_81 |
| GroupedDBID | AAYXX ALMA_UNASSIGNED_HOLDINGS CITATION GROUPED_DOAJ M1Z M~E OK1 KROLR ACYCR |
| ID | FETCH-LOGICAL-c2731-26e3fc24dea5574ee4d6492eac5730115ba036558b1735fab2c9a98a128b376b3 |
| IEDL.DBID | DOA |
| ISSN | 2671-4639 |
| IngestDate | Wed Sep 10 03:51:38 EDT 2025 Wed Aug 27 01:31:22 EDT 2025 Sun Nov 10 04:24:29 EST 2024 Tue Jul 01 02:41:09 EDT 2025 Thu Apr 24 23:05:39 EDT 2025 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 2 |
| Keywords | myoblasts platelet-derived growth factor alpha leydig cells testis receptors |
| Language | English |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c2731-26e3fc24dea5574ee4d6492eac5730115ba036558b1735fab2c9a98a128b376b3 |
| Notes | https://www.e-jarb.org/journal/view.html?uid=2692&vmd=Full |
| ORCID | 0000-0002-1585-6886 0000-0001-7402-7298 0000-0003-4469-7201 0000-0002-0357-9666 0000-0001-9969-0117 0000-0003-1068-9334 |
| OpenAccessLink | https://doaj.org/article/ff27b4b493934320807654880b71a539 |
| PageCount | 7 |
| ParticipantIDs | nrf_kci_oai_kci_go_kr_ARTI_10537778 doaj_primary_oai_doaj_org_article_ff27b4b493934320807654880b71a539 koreascholar_journals_437077 crossref_primary_10_12750_JARB_39_2_81 crossref_citationtrail_10_12750_JARB_39_2_81 |
| ProviderPackageCode | CITATION AAYXX |
| PublicationCentury | 2000 |
| PublicationDate | 20240630 |
| PublicationDateYYYYMMDD | 2024-06-30 |
| PublicationDate_xml | – month: 06 year: 2024 text: 20240630 day: 30 |
| PublicationDecade | 2020 |
| PublicationTitle | Journal of animal reproduction & biotechnology (Online) |
| PublicationYear | 2024 |
| Publisher | 한국동물생명공학회(구 한국수정란이식학회) The Korean Society of Animal Reproduction and Biotechnology 사단법인 한국동물생명공학회 |
| Publisher_xml | – name: 한국동물생명공학회(구 한국수정란이식학회) – name: The Korean Society of Animal Reproduction and Biotechnology – name: 사단법인 한국동물생명공학회 |
| References | ref13 ref12 ref15 ref14 ref11 ref10 ref2 ref1 ref17 ref16 ref18 ref8 ref7 ref9 ref4 ref3 ref6 ref5 |
| References_xml | – ident: ref3 doi: 10.1111/j.1447-0578.2006.00118.x – ident: ref2 doi: 10.1101/gad.1052503 – ident: ref18 doi: 10.1016/j.mce.2021.111179 – ident: ref7 doi: 10.1242/dev.00721 – ident: ref8 doi: 10.1016/j.bbrc.2022.03.149 – ident: ref9 doi: 10.12750/JARB.37.2.87 – ident: ref6 doi: 10.1371/journal.pone.0182265 – ident: ref13 doi: 10.1101/2023.07.21.549565 – ident: ref15 doi: 10.1016/S1043-2760(01)00518-5 – ident: ref10 doi: 10.1111/j.1582-4934.2011.01510.x – ident: ref16 doi: 10.12750/JARB.34.4.311 – ident: ref1 doi: 10.1016/j.diff.2022.02.006 – ident: ref11 doi: 10.1016/j.reprotox.2023.108497 – ident: ref14 doi: 10.1002/mrd.23574 – ident: ref5 doi: 10.1083/jcb.131.4.1105 – ident: ref17 doi: 10.12750/JARB.38.4.291 – ident: ref12 doi: 10.7554/eLife.36865 – ident: ref4 doi: 10.1083/jcb.149.5.1019 |
| SSID | ssib055788481 ssj0002785674 ssib053376818 |
| Score | 2.2599795 |
| Snippet | Background: Platelet-derived growth factor receptor alpha (PDGFRα) is essential for various biological processes, including fetal Leydig cell differentiation.... |
| SourceID | nrf doaj koreascholar crossref |
| SourceType | Open Website Aggregation Database Enrichment Source Index Database |
| StartPage | 81 |
| SubjectTerms | leydig cells myoblasts platelet-derived growth factor alpha receptors testis 축산학 |
| TableOfContents | ABSTRACT INTRODUCTION MATERIALS AND METHODS Animal Genotyping Hematoxylin and eosin (H&E) staining Immunohistochemistry (IHC) RESULTS Genotyping of PDGFRαEGFP mice Identification of PDGFRα in PDGFRαEGFP mousetesticular tissue Localization of c-Kit, ANO-1, and TASK1 in PDGFRα+cells DISCUSSION REFERENCES |
| Title | Analysis of platelet-derived growth factor receptor alpha expression in adult mouse testis |
| URI | http://db.koreascholar.com/Article/Detail/437077 https://doaj.org/article/ff27b4b493934320807654880b71a539 https://www.kci.go.kr/kciportal/ci/sereArticleSearch/ciSereArtiView.kci?sereArticleSearchBean.artiId=ART003095177 |
| Volume | 39 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| ispartofPNX | 한국동물생명공학회지, 2024, 39(2), , pp.81-87 |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1NT9wwELUqTu2hoh-IBVpZatUTgdixY-e4Wy2iSPRQFQlxsezYXmBX2dWyRZz62ztjh-32UHHhkkSREzkzY88bZ_yGkM-N8JpXsS4EY7YQKorCCd8WZabBbbwLuN5x_r0-vRBnl_Jyo9QX5oRleuAsuOMYuXLCCXgO90ACwFFY6lyXTjErq7R1r2zKjWAKLAkwDMDov7OuBLtc88bf5t9tsk4UzbxWEESBn-4JOJHv_Phs-GN0hPtYjjT7x2ElXn_wUdM5pozn8BO8UreMG17pZJu87uEkHebPeENehO4teTWcLHtKjfCOXD3yjtB5pIsZYEtQVeHB8u6DpxMIw1fXNJfdoTD9hQVepC24NDz0abIdveloouqguFQQ6ArJOe7ek4uT8c-vp0VfUaFoAaawgtehii0XPlgQiQhB-Fo0HCZfmUa6dBY8mpTaMVXJaB1vG9toC07MgURdtUO2unkXdglloBjGvfLKBqG0103NIit9XUYRWLQDcvgoNtP2dONY9WJmMOxAKRuUsqkaw41mA_Jl3XyReTb-13CEOlg3QnrsdAOMxvRGY54ymgE52NSg6cftnRGVKpUakE-gUTNtb9Lr8TyZm-nSQIzxDbolK6WU3nuOnuyTlxxgUs5APCBbq-Wv8AFgzsp9TBYNx_Pf4z87vPLK |
| linkProvider | Directory of Open Access Journals |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Analysis+of+platelet-derived+growth+factor+receptor+alpha+expression+in+adult+mouse+testis&rft.jtitle=Journal+of+animal+reproduction+%26+biotechnology+%28Online%29&rft.au=%EC%9A%B0%EB%AF%BC%EC%84%9D&rft.au=%EA%B9%80%EC%9D%80%EC%A7%84&rft.au=%EC%9D%B4%EB%8F%99%EA%B7%BC&rft.au=%EC%9D%B4%EC%A0%95%EC%9D%80&rft.date=2024-06-30&rft.pub=%EC%82%AC%EB%8B%A8%EB%B2%95%EC%9D%B8+%ED%95%9C%EA%B5%AD%EB%8F%99%EB%AC%BC%EC%83%9D%EB%AA%85%EA%B3%B5%ED%95%99%ED%9A%8C&rft.issn=2671-4639&rft.eissn=2671-4663&rft.spage=81&rft.epage=87&rft_id=info:doi/10.12750%2FJARB.39.2.81&rft.externalDBID=n%2Fa&rft.externalDocID=oai_kci_go_kr_ARTI_10537778 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2671-4639&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2671-4639&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2671-4639&client=summon |