Abortive phage-infection and UV-protection markers on Col I plasmids from epidemic strains of Salmonella

• Published in: Journal of medical microbiology Vol. 25; no. 3; pp. 221 - 225
• Main Author: Barker, Ruth M
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.03.1988; Society for General Microbiology
• Subjects: Bacteriology; Biological and medical sciences; Escherichia coli - genetics; Escherichia coli - radiation effects; Fundamental and applied biological sciences. Psychology; Humans; Microbiology; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains; Plasmids; Salmonella - genetics; Salmonella - radiation effects; Salmonella Infections - microbiology; Salmonella Phages - genetics; Salmonella typhimurium - genetics; Salmonella typhimurium - isolation & purification; Salmonella typhimurium - radiation effects; Ultraviolet Rays; Epidemic; Human origin; Salmonella; Biological marker; Strain; Virus; Infection; Plasmid; Ultraviolet radiation; Bacteria; Escherichieae; Protection; Enterobacteriaceae; Phage
• DOI: 10.1099/00222615-25-3-221
• ISSN: 0022-2615

Department of Microbiology, University of Dundee Medical School, Ninewells Hospital, Dundee DD1 9SY Received June 8, 1987 Accepted June 15, 1987 Cultures of Escherichia coli carrying ColI plasmids received in conjugation from strains of Salmonella typhimurium and S. agona were examined for abortive infection (Abi) of phage BF23 and for enhanced resistance to the lethal action of UV-irradiation (Uvr). The Abi character of stored cultures of E. coli was also compared with the reaction of the same stock culture tested 5 years before. Seven of the eight potential types differentiated by three characters were represented among 160 ColI plasmids: ColIa Abi + Uvr + (3 plasmids), ColIa Abi – Uvr + (1), ColIa Abi – Uvr – (2), ColIb Abi + Uvr + (85), ColIb Abi + Uvr – (5), ColIb Abi – Uvr + (4), ColIb Abi – Uvr – (60). Recognition that different plasmid types could be carried by strains of a clone proved useful in the interpretation of the epidemic spread of strains of S. typhimurium of phage type/biotype 141/9f in Scotland and in tracing the ancestry of a recently emerged rhamnose non-fermenting mutant strain of S. agona.