Immuno-radiometric assays for human salivary acidic proline-rich proteins and their N- and C-terminal fragments

An immuno-radiometric assay was developed for acidic proline-rich proteins from human saliva, and assays designed which specifically detect the N- or C-terminal parts of the proteins. The immuno-radiometric assay depends on the binding of antigen to paper discs coated with specific antibodies. Subse...

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Bibliographic Details
Published inArchives of oral biology Vol. 28; no. 1; pp. 13 - 17
Main Authors Bennick, A., Madapallimattam, G.
Format Journal Article
LanguageEnglish
Published England Elsevier Ltd 1983
Subjects
Dentistry
Humans
Peptide Fragments - analysis
Peptides - analysis
Proline-Rich Protein Domains
Radioimmunoassay - methods
Saliva - analysis
Salivary Proline-Rich Proteins
Salivary Proteins and Peptides - analysis
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Summary:An immuno-radiometric assay was developed for acidic proline-rich proteins from human saliva, and assays designed which specifically detect the N- or C-terminal parts of the proteins. The immuno-radiometric assay depends on the binding of antigen to paper discs coated with specific antibodies. Subsequently, the discs are incubated with radioactive specific antibodies. The amount of radioactive antibodies bound to the discs depends on the amount of antigen already adhering to the discs.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0003-9969
1879-1506
DOI:10.1016/0003-9969(83)90021-3