Desulfosporosinus metallidurans sp. nov., an acidophilic, metal-resistant sulfate-reducing bacterium from acid mine drainage
A novel, spore-forming, acidophilic and metal-resistant sulfate-reducing bacterium, strain OL T , was isolated from a microbial mat in a tailing dam at a gold ore mining site. Cells were slightly curved immotile rods, 0.5 µm in diameter and 2.0–3.0 µm long. Cells were stained Gram-negative, despite...
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Published in | International journal of systematic and evolutionary microbiology Vol. 71; no. 7 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
01.01.2021
|
Online Access | Get full text |
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Summary: | A novel, spore-forming, acidophilic and metal-resistant sulfate-reducing bacterium, strain OL
T
, was isolated from a microbial mat in a tailing dam at a gold ore mining site. Cells were slightly curved immotile rods, 0.5 µm in diameter and 2.0–3.0 µm long. Cells were stained Gram-negative, despite the Gram-positive cell structure revealed by electron microscopy of ultrathin layers. OL
T
grew at pH 4.0–7.0 with an optimum at 5.5. OL
T
utilised H
2
, lactate, pyruvate, malate, formate, propionate, ethanol, glycerol, glucose, fructose, sucrose, peptone and tryptone as electron donors for sulfate reduction. Sulfate, sulfite, thiosulfate, nitrate and fumarate were used as electron acceptors in the presence of lactate. Elemental sulfur, iron (III), and arsenate did not serve as electron acceptors. The major cellular fatty acids were C
16:1
ω7
c
(39.0 %) and C
16 : 0
(12.1 %). The draft genome of OL
T
was 5.29 Mb in size and contained 4909 protein-coding genes. The 16S rRNA gene sequence placed OL
T
within the phylum
Firmicutes
, class
Clostridia
, family
Peptococcaceae
, genus
Desulfosporosinus. Desulfosporosinus nitroreducens
59.4B
T
was the closest relative with 97.6 % sequence similarity. On the basis of phenotypic and phylogenetic characteristics, strain OL
T
represents a novel species within the genus
Desulfosporosinus
, for which we propose the name
Desulfosporosinus metallidurans
sp. nov. with the type strain OL
T
(=DSM 104464
T
=VKM В−3021
T
). |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1466-5026 1466-5034 |
DOI: | 10.1099/ijsem.0.004876 |