On-line coupling of micellar electrokinetic chromatography to electrospray mass spectrometry

The possibility of selectivity enhancement in capillary electrophoresis-mass spectrometry (CE-MS) by hyphenating micellar electrokinetic chromatography (MEKC) and electrospray mass spectrometry (MS) is described for two quaternary ammonium compounds. Direct coupling of MEKC to MS is hazardous becaus...

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Published inJournal of Chromatography A Vol. 712; no. 1; pp. 219 - 225
Main Authors Lamoree, M.H., Tjaden, U.R., van der Greef, J.
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 29.09.1995
Subjects
Buffers
Capillary Action
Chromatography - methods
Electrophoresis
Kinetics
Mass Spectrometry - methods
Micelles
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Abstract The possibility of selectivity enhancement in capillary electrophoresis-mass spectrometry (CE-MS) by hyphenating micellar electrokinetic chromatography (MEKC) and electrospray mass spectrometry (MS) is described for two quaternary ammonium compounds. Direct coupling of MEKC to MS is hazardous because of the contamination of the ion source due to presence of an excess of micelle forming agent in the MEKC buffer. Therefore, a coupled-capillary setup with the possibilities of voltage switching and buffer renewal has been designed. Such a system allows on-line heartcutting of the zones of interest in the MEKC capillary with subsequent transfer via a second capillary to the mass spectrometer.
AbstractList The possibility of selectivity enhancement in capillary electrophoresis-mass spectrometry (CE-MS) by hyphenating micellar electrokinetic chromatography (MEKC) and electrospray mass spectrometry (MS) is described for two quaternary ammonium compounds. Direct coupling of MEKC to MS is hazardous because of the contamination of the ion source due to presence of an excess of micelle forming agent in the MEKC buffer. Therefore, a coupled-capillary setup with the possibilities of voltage switching and buffer renewal has been designed. Such a system allows on-line heartcutting of the zones of interest in the MEKC capillary with subsequent transfer via a second capillary to the mass spectrometer.
Author Tjaden, U.R.
Lamoree, M.H.
van der Greef, J.
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Snippet The possibility of selectivity enhancement in capillary electrophoresis-mass spectrometry (CE-MS) by hyphenating micellar electrokinetic chromatography (MEKC)...
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SubjectTerms Buffers
Capillary Action
Chromatography - methods
Electrophoresis
Kinetics
Mass Spectrometry - methods
Micelles
Title On-line coupling of micellar electrokinetic chromatography to electrospray mass spectrometry
URI https://dx.doi.org/10.1016/0021-9673(95)00507-J
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