The interaction of von Willebrand factor (vWf) with collagen: investigation of vWf-binding sites in the collagen molecule

• Published in: Thrombosis and haemostasis Vol. 59; no. 2; p. 186
• Main Authors: Fitzsimmons, C M, Cockburn, C G, Hornsey, V, Prowse, C V, Barnes, M J
• Format: Journal Article
• Language: English
• Published: Germany 1988
• Subjects: Amino Acids - metabolism; Binding Sites; Collagen - metabolism; Cyanogen Bromide; Fibronectins - metabolism; In Vitro Techniques; Peptide Fragments - isolation & purification; Peptide Fragments - metabolism; von Willebrand Factor - metabolism

Following fragmentation of the collagen molecule, we have examined the ability of the isolated fragments to bind vWf. In view of the importance of collagen tertiary and quaternary structure for binding, fragments were first renatured to restore triple-helical conformation and then polymerized. Resul...