Azide-alkyne "click" reaction performed on oligonucleotides with the universal nucleoside 7-octadiynyl-7-deaza-2′-deoxyinosine

• Published in: Nucleic Acids Symposium Series Vol. 52; no. 1; pp. 471 - 472
• Main Authors: Ming, Xin, Leonard, Peter, Heindl, Dieter, Seela, Frank
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 2008
• Subjects: DNA - chemistry; Fluorescent Dyes - chemistry; Inosine - analogs & derivatives; Inosine - chemistry; Oligonucleotides - chemistry

Abstract Oligonucleotides containing 7-substituted 7-deaza-2′- deoxyinosine derivatives bearing alkynyl groups were prepared. The octa-1,7-diynyl derivative was functionalized with the non-fluorescent 3- azidocoumarin by the Huisgen-Sharpless-Meldal cycloaddition to afford a highly fluorescent oligo...