A molecular and parasitological survey on cutaneous leishmaniasis patients from historical city of Kashan in Isfahan province, center of Iran
To study cutaneous leishmaniasis (CL) for identifying the dominant Leishmania species on CL patients referred to medical health centers of historical Kashan city and suburbs located in Isfahan province in central part of Iran during 2010 to 2011. From 137 CL cases, were microscopically positive, the...
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| Published in | Asian Pacific journal of tropical disease Vol. 2; no. 6; pp. 421 - 425 |
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| Main Authors | , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Elsevier B.V
01.12.2012
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| Abstract | To study cutaneous leishmaniasis (CL) for identifying the dominant Leishmania species on CL patients referred to medical health centers of historical Kashan city and suburbs located in Isfahan province in central part of Iran during 2010 to 2011.
From 137 CL cases, were microscopically positive, the skin lesion serosity materials of 103 cases were cultured in monophasic culture media (RPMI 1 640). We used the PCR-RFLP method for characterization the Leishmania isolates, by using specific internal transcribed spacer (ITS1) primers and HAEШ as the restriction fast enzyme. DNA was extracted from 63 samples.
L. tropica is main species in 58 (92.1%) cases and L. major is identified in 5 (7.9%) cases. Indeed randomly two isolates were the species characterized as L. major produced ulcer at the base tail of BALB/c mice after 3 weeks but from three L. tropica isolates none of them produced any lesion during 6 months post inoculation.
The parasitological, epidemiological aspect and molecular methods of this study showed that, Kashan and suburb are anthroponetic CL area despite this city located in Isfahan province as an ancient focus of zoonotic CL in Iran. |
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| AbstractList | To study cutaneous leishmaniasis (CL) for identifying the dominant Leishmania species on CL patients referred to medical health centers of historical Kashan city and suburbs located in Isfahan province in central part of Iran during 2010 to 2011.
From 137 CL cases, were microscopically positive, the skin lesion serosity materials of 103 cases were cultured in monophasic culture media (RPMI 1 640). We used the PCR-RFLP method for characterization the Leishmania isolates, by using specific internal transcribed spacer (ITS1) primers and HAEШ as the restriction fast enzyme. DNA was extracted from 63 samples.
L. tropica is main species in 58 (92.1%) cases and L. major is identified in 5 (7.9%) cases. Indeed randomly two isolates were the species characterized as L. major produced ulcer at the base tail of BALB/c mice after 3 weeks but from three L. tropica isolates none of them produced any lesion during 6 months post inoculation.
The parasitological, epidemiological aspect and molecular methods of this study showed that, Kashan and suburb are anthroponetic CL area despite this city located in Isfahan province as an ancient focus of zoonotic CL in Iran. |
| Author | hajjaran, Homa Shiee, Mohammad Reza Afzali, Hassan Doroodgar, Abbas Shirzadi, Mohammad Reza Teimouri, Aref Mohebali, Mehdi saadat, Masoomeh Hashemi |
| Author_xml | – sequence: 1 givenname: Mohammad Reza surname: Shiee fullname: Shiee, Mohammad Reza organization: Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran – sequence: 2 givenname: Homa surname: hajjaran fullname: hajjaran, Homa email: hajaranh@tums.ac.ir, hhajjaran@yahoo.com organization: Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran – sequence: 3 givenname: Mehdi surname: Mohebali fullname: Mohebali, Mehdi organization: Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran – sequence: 4 givenname: Abbas surname: Doroodgar fullname: Doroodgar, Abbas organization: Department of Medical Parasitology, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran – sequence: 5 givenname: Masoomeh Hashemi surname: saadat fullname: saadat, Masoomeh Hashemi organization: Department of Medical Parasitology, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran – sequence: 6 givenname: Aref surname: Teimouri fullname: Teimouri, Aref organization: Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran – sequence: 7 givenname: Hassan surname: Afzali fullname: Afzali, Hassan organization: Department of Infection Diseases, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran – sequence: 8 givenname: Mohammad Reza surname: Shirzadi fullname: Shirzadi, Mohammad Reza organization: Zoonoses Control Unit, Center of Communicable Diseases Control, Ministry of Health, Treatment and Medical Education, Tehran, Iran |
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| CitedBy_id | crossref_primary_10_1007_s11686_022_00575_9 crossref_primary_10_1093_trstmh_trac081 crossref_primary_10_3389_fpubh_2021_661674 crossref_primary_10_1016_S1995_7645_13_60116_9 crossref_primary_10_1016_S2222_1808_14_60673_3 crossref_primary_10_1155_2013_789326 crossref_primary_10_1016_j_micpath_2020_104721 crossref_primary_10_14202_vetworld_2018_700_705 crossref_primary_10_3923_pjbs_2016_136_142 crossref_primary_10_1051_parasite_2022047 crossref_primary_10_1016_S1995_7645_13_60034_6 crossref_primary_10_1016_S1995_7645_13_60066_8 crossref_primary_10_1007_s00580_018_2658_9 |
| Cites_doi | 10.1016/j.actatropica.2006.01.010 10.1179/136485907X176436 10.1016/S2222-1808(12)60056-5 10.3347/kjp.2006.44.4.355 10.1016/j.exppara.2009.10.004 10.1002/jcla.20377 10.1016/S1995-7645(11)60045-X 10.1016/S2222-1808(12)60057-7 10.1111/j.1365-3156.2011.02729.x 10.1016/S1995-7645(10)60220-9 10.1016/S1995-7645(10)60192-7 10.1016/j.actatropica.2011.05.010 10.1016/j.exppara.2009.01.015 10.1007/s13149-010-0044-1 10.1590/S1413-86702011000100004 10.1111/j.1600-0560.2011.01861.x |
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