Thyroid growth stimulating activity in highly purified IgG-fractions of patients with nonimmune thyroid diseases

Two different proliferation assays have been used to measure the proliferative potential of IgG-fractions from 57 patients with nontoxic goiter of an iodine-deficient area: primary human thyroid epithelial cells (TEC) and the thouroughly investigated FRTL-5 cell line. IgG-fractions from patients wit...

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Bibliographic Details
Published inJournal of endocrinological investigation Vol. 12; no. 9; p. 631
Main Authors Oethinger, M D, Wenzel, B E, Scriba, P C
Format Journal Article
LanguageEnglish
Published Italy 01.10.1989
Subjects
Adolescent
Adult
Aged
Biological Assay
Cell Division
Cells, Cultured
Epithelial Cells
Female
Humans
Immunoglobulin G - analysis
Immunoglobulin G - physiology
Immunoglobulins, Thyroid-Stimulating
Male
Middle Aged
Thyroid Diseases - immunology
Thyroid Gland - cytology
Thyroid Gland - growth & development
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Summary:Two different proliferation assays have been used to measure the proliferative potential of IgG-fractions from 57 patients with nontoxic goiter of an iodine-deficient area: primary human thyroid epithelial cells (TEC) and the thouroughly investigated FRTL-5 cell line. IgG-fractions from patients with nontoxic goiter (n = 30), nontoxic recurrent goiter (n = 8), toxic-nodular goiter (n = 15) and carcinoma of the thyroid (n = 4) were highly purified on DEAE-Sepharose and additionally Protein A-Sepharose in some cases. The two proliferation assays gave contradictory results: primary cultures of human thyroid epithelial cells (TEC) could not be stimulated by any of the patient's IgG-fractions nor by bTSH. The FRTL-5 cells, however, were stimulated with 10 microU/ml bTSH by 326% +/- 96% (range: 222% - 497%, p less than 0.001). In one experimental series, 72% of all patients exceeded mean + 2 SD of normal controls, when the stimulation index was referred to the effect of bTSH (NTG: 77%, Rec. G.: 88%, Tox. G.: 53%, Ca. thyroid: 75%). With a different method of calculation - stimulation index referred to the basal value - the number of patients above mean + 2 SD of normal controls decreased to 30% (NTG: 33%, Rec. G.: 12.5%, Tox. G.: 33%, Ca. thyroid: 25%). Statistical analysis, however, of results of different patient groups compared to the normal control group failed to show any significance.
ISSN:0391-4097
1720-8386
DOI:10.1007/BF03350024