Significant reduction of cell invasiveness in nanoneedle insertion into a living cell with an electron-beam-deposited probe: impacts of probe geometry, speed and vibration

• Published in: Nanoscale Vol. 17; no. 12; pp. 7342 - 735
• Main Authors: Alam, Mohammad Shahidul, Penedo, Marcos, Ichikawa, Takehiko, Hosain, Mohammad Mubarak, Matsumoto, Kyosuke, Miyazawa, Keisuke, Fukuma, Takeshi
• Format: Journal Article
• Language: English
• Published: England Royal Society of Chemistry 24.03.2025
• Subjects: Animals; Atomic force microscopy; Carbon; Carbon - chemistry; Cell Membrane - chemistry; Cell Membrane - metabolism; Cell membranes; Damage; Deformation effects; Diameters; Electron beams; Electrons; Humans; Ion beams; Microscopy, Atomic Force; Nanotechnology - instrumentation; Vibration

Intracellular probing of living cells using atomic force microscopy (AFM) has advanced significantly, but it requires specially designed nanoprobes to achieve precision and minimize damage. The development of focused ion beam (FIB)-milled nanoprobes enabled this progress, allowing researchers to fab...