Gene characterization of Bradyrhizobium spp. strains contrasting in biological nitrogen fixation efficiency in soybean
Bacteria from genus Bradyrhizobium can establish symbiosis with soybean and supply the plant nitrogen demands via biological nitrogen fixation (BNF). This study aimed to characterize genes related to BNF efficiency in B. japonicum strains contrasting in BNF efficiency. These gene sequences were prev...
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Published in | Semina. Ciências agrárias : revista cultural e científica da Universidade Estadual de Londrina Vol. 41; no. 6supl2; pp. 3067 - 3080 |
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Format | Journal Article |
Language | English |
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01.01.2020
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Abstract | Bacteria from genus Bradyrhizobium can establish symbiosis with soybean and supply the plant nitrogen demands via biological nitrogen fixation (BNF). This study aimed to characterize genes related to BNF efficiency in B. japonicum strains contrasting in BNF efficiency. These gene sequences were previously identified in B. japonicum (strain S370) as probably related to the BNF efficiency in soybean using a DNA subtractive technique. These genes were amplified with primers based on B. japonicum USDA110 genome. The PCR products were digested with restriction endonucleases and the RFLP products were analyzed by horizontal electrophoresis. Among the four genes, only blr3208 and blr4511 amplified for most of the strains. Neither polymorphism of the restriction profile of blr3208 and blr4511 genes nor with endonuclease for PCR-RFLP was observed. The contrasting strains had blr3208 and blr4511 genes sequenced and the multiple alignment analysis of nucleotide sequences showed the presence of preserved internal regions, confirming the analysis with PCR-RFLP. The blr3208 and blr4511 genes are highly conserved among B. japonicum strains, which may be related to adaptive function during the evolutionary process of Bradyrhizobium genus. |
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AbstractList | Bacteria from genus Bradyrhizobium can establish symbiosis with soybean and supply the plant nitrogen demands via biological nitrogen fixation (BNF). This study aimed to characterize genes related to BNF efficiency in B. japonicum strains contrasting in BNF efficiency. These gene sequences were previously identified in B. japonicum (strain S370) as probably related to the BNF efficiency in soybean using a DNA subtractive technique. These genes were amplified with primers based on B. japonicum USDA110 genome. The PCR products were digested with restriction endonucleases and the RFLP products were analyzed by horizontal electrophoresis. Among the four genes, only blr3208 and blr4511 amplified for most of the strains. Neither polymorphism of the restriction profile of blr3208 and blr4511 genes nor with endonuclease for PCR-RFLP was observed. The contrasting strains had blr3208 and blr4511 genes sequenced and the multiple alignment analysis of nucleotide sequences showed the presence of preserved internal regions, confirming the analysis with PCR-RFLP. The blr3208 and blr4511 genes are highly conserved among B. japonicum strains, which may be related to adaptive function during the evolutionary process of Bradyrhizobium genus. |
Author | Paccola-Meirelles, Luzia Doretto Oliveira Filho, Olavo Bilac Quaresma de Valle, Juliana Silveira do Colauto, Nelson Barros Medeiros, Camila de Barcellos, Fernando Gomes Freitas, Janyeli Dorini Silva de Pereira, Gilberto Aguiar Linde, Giani Andrea |
Author_xml | – sequence: 1 givenname: Camila de orcidid: 0000-0002-2432-1250 surname: Medeiros fullname: Medeiros, Camila de – sequence: 2 givenname: Gilberto Aguiar orcidid: 0000-0002-2338-5586 surname: Pereira fullname: Pereira, Gilberto Aguiar – sequence: 3 givenname: Janyeli Dorini Silva de orcidid: 0000-0002-8350-6650 surname: Freitas fullname: Freitas, Janyeli Dorini Silva de – sequence: 4 givenname: Olavo Bilac Quaresma de orcidid: 0000-0001-7259-2066 surname: Oliveira Filho fullname: Oliveira Filho, Olavo Bilac Quaresma de – sequence: 5 givenname: Juliana Silveira do orcidid: 0000-0002-9463-5378 surname: Valle fullname: Valle, Juliana Silveira do – sequence: 6 givenname: Giani Andrea orcidid: 0000-0003-1220-2032 surname: Linde fullname: Linde, Giani Andrea – sequence: 7 givenname: Luzia Doretto orcidid: 0000-0001-6090-2872 surname: Paccola-Meirelles fullname: Paccola-Meirelles, Luzia Doretto – sequence: 8 givenname: Nelson Barros orcidid: 0000-0003-4390-8302 surname: Colauto fullname: Colauto, Nelson Barros – sequence: 9 givenname: Fernando Gomes orcidid: 0000-0002-8781-3754 surname: Barcellos fullname: Barcellos, Fernando Gomes |
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