Augmentation of antibody-dependent cell-mediated hepatocytotoxicity by culture supernatant from lipopolysaccharide (LPS)-activated Kupffer cells
A significant liver cell damage was induced when the isolated liver cells coated with the specific antibody against the liver cell membrane were cultured with peripheral blood mononuclear cells and their culture fluid was added to the isolated liver cells. These were demonstrated by measuring the de...
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| Published in | Kanzo Vol. 25; no. 12; pp. 1552 - 1556 |
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| Main Authors | , , , , , , |
| Format | Journal Article |
| Language | Japanese |
| Published |
The Japan Society of Hepatology
1984
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| Subjects | |
| Online Access | Get full text |
| ISSN | 0451-4203 1881-3593 |
| DOI | 10.2957/kanzo.25.1552 |
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| Abstract | A significant liver cell damage was induced when the isolated liver cells coated with the specific antibody against the liver cell membrane were cultured with peripheral blood mononuclear cells and their culture fluid was added to the isolated liver cells. These were demonstrated by measuring the decrease in protein synthesis of liver cells. These liver cell injuries by antibody-dependent cell-mediated cytotoxicity (ADCC) were significantly potentiated by adding the culture supernatant of LPS-activated Kupffer cells to the effector cells. Since interleukin-1 activity was detected in the culture supernatant of LPS-activated Kupffer cells, it is likely that augmentation of ADCC by activated Kupffer cells is attributable to interleukin-1 at least partially. These results suggest a possibility that Kupffer cells may modulate the ADCC reaction. |
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| AbstractList | A significant liver cell damage was induced when the isolated liver cells coated with the specific antibody against the liver cell membrane were cultured with peripheral blood mononuclear cells and their culture fluid was added to the isolated liver cells. These were demonstrated by measuring the decrease in protein synthesis of liver cells. These liver cell injuries by antibody-dependent cell-mediated cytotoxicity (ADCC) were significantly potentiated by adding the culture supernatant of LPS-activated Kupffer cells to the effector cells. Since interleukin-1 activity was detected in the culture supernatant of LPS-activated Kupffer cells, it is likely that augmentation of ADCC by activated Kupffer cells is attributable to interleukin-1 at least partially. These results suggest a possibility that Kupffer cells may modulate the ADCC reaction. |
| Author | YAMAMOTO, Sukeo MIYAJIMA, Keiji ARAI, Takayuki MORISAWA, Seiji KATOH, Hiroko TSUTSUI, Hiroko MIZOGUCHI, Yasuhiro |
| Author_xml | – sequence: 1 fullname: MIZOGUCHI, Yasuhiro organization: The Third Department of Internal Medicine, Osaka City University Medical School – sequence: 1 fullname: ARAI, Takayuki organization: The Third Department of Internal Medicine, Osaka City University Medical School – sequence: 1 fullname: MORISAWA, Seiji organization: The First Department of Biochemistry, Osaka City University Medical School – sequence: 1 fullname: YAMAMOTO, Sukeo organization: The Third Department of Internal Medicine, Osaka City University Medical School – sequence: 1 fullname: TSUTSUI, Hiroko organization: The Third Department of Internal Medicine, Osaka City University Medical School – sequence: 1 fullname: KATOH, Hiroko organization: The Third Department of Internal Medicine, Osaka City University Medical School – sequence: 1 fullname: MIYAJIMA, Keiji organization: The Third Department of Internal Medicine, Osaka City University Medical School |
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| References | 3) 沢井寛子,筒井ひろ子,阪上吉秀,他:ADCCによる免疫学的肝細胞障害のマクロファージ依存性.消化器と免疫9: 144-148, 1982 4) Richman LK, Klingenstein RJ, Richman JILLA,: The murine Kupffer cell I. Characterization of the cell serving accessory function in antigen specific T cell proliferation. J Immunol 123: 2602-2609, 1979 8) Munthe-Kass AC, Berg T, Seglen PO,: Mass isolation and culture of rat Kupffer cells. J Exp Med 141: 1-10, 1975 5) Nadler PI, Klingenstein RJ, Richman LK,: The murine Kupffer cell II. Accessory cell function in in vitro primary antibody responses, mitogen-induced proliferation, and stimulation of mixed lymphocyte responses. J Immunol 125: 2521-2525, 1980 7) 加藤寛子,溝口靖紘,箇井ひろ子,他:免疫性肝障害と性ホルモンに関する実験的研究.V.性ホルモンによるADCCの調節とインターロイキン-1の関与.日消誌81: 1398-1404, 1984 9) 熊谷勝男,伊藤恭吾,多田正人,他:再度,マクロファージの純粋分離法に関して.医学のあゆみ110: 611-624, 1979 12) Rogoff TM, Lipsky PE: Antigen presentation by isolated guinea pig Kupffer cells. J Immunol 124: 1740-1744, 1980 6) Mizoguchi Y, Shiba T, Monna T,: The possible involvement of Kupffer cell-mediated hepatocytotoxicity in th pathogenesis of liver injuries. Gastroenterologia Jpn 16: 377-383, 1981 10) Bellemann P, Gebhardt R, Mecke D: An improved method for the isolation of hepatocytes from liver slice. Selective removed of trypanblue dyeable cells. Analytical Biochem 81: 408-415, 1977 11) Lachman LB, Hacker MP, Handschumacher RE: Partial purification of human lymphocyte-activating factor (LAF) by ultrafiltration and electrophoretic techniques. J Immunol 119: 2019-2023, 1977 13) Branfield JMB, Wells M: Liver disease caused by Lysosomal enzyme from Kupffer cells. Lancet II: 836-836, 1978 1) 溝口靖紘,志波孝,東森俊博,他:慢性活動性肝炎における肝特異抗原をめぐる免疫学的肝細胞障害の発生機構:特に活性化マクロファージおよびADCC反応を中心にして.日内誌69: 1609-1617, 1980 2) 溝口靖紘,阪上吉秀,志波孝,他:ADCC反応による肝細胞障害機序.I. K-cellより産生分泌される細胞障害因子の部分精製.肝臓22: 1257-1263, 1981 |
| References_xml | – reference: 6) Mizoguchi Y, Shiba T, Monna T,: The possible involvement of Kupffer cell-mediated hepatocytotoxicity in th pathogenesis of liver injuries. Gastroenterologia Jpn 16: 377-383, 1981 – reference: 1) 溝口靖紘,志波孝,東森俊博,他:慢性活動性肝炎における肝特異抗原をめぐる免疫学的肝細胞障害の発生機構:特に活性化マクロファージおよびADCC反応を中心にして.日内誌69: 1609-1617, 1980 – reference: 2) 溝口靖紘,阪上吉秀,志波孝,他:ADCC反応による肝細胞障害機序.I. K-cellより産生分泌される細胞障害因子の部分精製.肝臓22: 1257-1263, 1981 – reference: 12) Rogoff TM, Lipsky PE: Antigen presentation by isolated guinea pig Kupffer cells. J Immunol 124: 1740-1744, 1980 – reference: 7) 加藤寛子,溝口靖紘,箇井ひろ子,他:免疫性肝障害と性ホルモンに関する実験的研究.V.性ホルモンによるADCCの調節とインターロイキン-1の関与.日消誌81: 1398-1404, 1984 – reference: 4) Richman LK, Klingenstein RJ, Richman JILLA,: The murine Kupffer cell I. Characterization of the cell serving accessory function in antigen specific T cell proliferation. J Immunol 123: 2602-2609, 1979 – reference: 8) Munthe-Kass AC, Berg T, Seglen PO,: Mass isolation and culture of rat Kupffer cells. J Exp Med 141: 1-10, 1975 – reference: 5) Nadler PI, Klingenstein RJ, Richman LK,: The murine Kupffer cell II. Accessory cell function in in vitro primary antibody responses, mitogen-induced proliferation, and stimulation of mixed lymphocyte responses. J Immunol 125: 2521-2525, 1980 – reference: 9) 熊谷勝男,伊藤恭吾,多田正人,他:再度,マクロファージの純粋分離法に関して.医学のあゆみ110: 611-624, 1979 – reference: 3) 沢井寛子,筒井ひろ子,阪上吉秀,他:ADCCによる免疫学的肝細胞障害のマクロファージ依存性.消化器と免疫9: 144-148, 1982 – reference: 10) Bellemann P, Gebhardt R, Mecke D: An improved method for the isolation of hepatocytes from liver slice. Selective removed of trypanblue dyeable cells. Analytical Biochem 81: 408-415, 1977 – reference: 11) Lachman LB, Hacker MP, Handschumacher RE: Partial purification of human lymphocyte-activating factor (LAF) by ultrafiltration and electrophoretic techniques. J Immunol 119: 2019-2023, 1977 – reference: 13) Branfield JMB, Wells M: Liver disease caused by Lysosomal enzyme from Kupffer cells. Lancet II: 836-836, 1978 |
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| SubjectTerms | antibody-depekdent cell-mediated cytotoxicity (ADCC) |
| Title | Augmentation of antibody-dependent cell-mediated hepatocytotoxicity by culture supernatant from lipopolysaccharide (LPS)-activated Kupffer cells |
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