Cingulin-nonmuscle myosin interaction plays a role in epithelial morphogenesis and cingulin nanoscale organization
Cingulin (CGN) tethers nonmuscle myosin 2B (NM2B; heavy chain encoded by MYH10) to tight junctions (TJs) to modulate junctional and apical cortex mechanics. Here, we studied the role of the CGN-nonmuscle myosin 2 (NM2) interaction in epithelial morphogenesis and nanoscale organization of CGN by expr...
Saved in:
Published in | Journal of cell science Vol. 137; no. 18 |
---|---|
Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
England
The Company of Biologists Ltd
15.09.2024
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | Cingulin (CGN) tethers nonmuscle myosin 2B (NM2B; heavy chain encoded by MYH10) to tight junctions (TJs) to modulate junctional and apical cortex mechanics. Here, we studied the role of the CGN-nonmuscle myosin 2 (NM2) interaction in epithelial morphogenesis and nanoscale organization of CGN by expressing wild-type and mutant CGN constructs in CGN-knockout Madin-Darby canine kidney (MDCK) epithelial cells. We show that the NM2-binding region of CGN is required to promote normal cyst morphogenesis of MDCK cells grown in three dimensions and to maintain the C-terminus of CGN in a distal position with respect to the ZO-2 (or TJP2)-containing TJ submembrane region, whereas the N-terminus of CGN is localized more proximal to the TJ membrane. We also show that the CGN mutant protein that causes deafness in human and mouse models is localized at TJs but does not bind to NM2B, resulting in decreased TJ membrane tortuosity. These results indicate that the interaction between CGN and NM2B regulates epithelial tissue morphogenesis and nanoscale organization of CGN and suggest that CGN regulates the auditory function of hair cells by organizing the actomyosin cytoskeleton to modulate the mechanics of the apical and junctional cortex. |
---|---|
AbstractList | Cingulin (CGN) tethers nonmuscle myosin 2B (NM2B; heavy chain encoded by MYH10) to tight junctions (TJs) to modulate junctional and apical cortex mechanics. Here, we studied the role of the CGN-nonmuscle myosin 2 (NM2) interaction in epithelial morphogenesis and nanoscale organization of CGN by expressing wild-type and mutant CGN constructs in CGN-knockout Madin-Darby canine kidney (MDCK) epithelial cells. We show that the NM2-binding region of CGN is required to promote normal cyst morphogenesis of MDCK cells grown in three dimensions and to maintain the C-terminus of CGN in a distal position with respect to the ZO-2 (or TJP2)-containing TJ submembrane region, whereas the N-terminus of CGN is localized more proximal to the TJ membrane. We also show that the CGN mutant protein that causes deafness in human and mouse models is localized at TJs but does not bind to NM2B, resulting in decreased TJ membrane tortuosity. These results indicate that the interaction between CGN and NM2B regulates epithelial tissue morphogenesis and nanoscale organization of CGN and suggest that CGN regulates the auditory function of hair cells by organizing the actomyosin cytoskeleton to modulate the mechanics of the apical and junctional cortex. Cingulin (CGN) tethers nonmuscle myosin 2B (NM2B; heavy chain encoded by MYH10 ) to tight junctions (TJs) to modulate junctional and apical cortex mechanics. Here, we studied the role of the CGN–nonmuscle myosin 2 (NM2) interaction in epithelial morphogenesis and nanoscale organization of CGN by expressing wild-type and mutant CGN constructs in CGN-knockout Madin–Darby canine kidney (MDCK) epithelial cells. We show that the NM2-binding region of CGN is required to promote normal cyst morphogenesis of MDCK cells grown in three dimensions and to maintain the C-terminus of CGN in a distal position with respect to the ZO-2 (or TJP2)-containing TJ submembrane region, whereas the N-terminus of CGN is localized more proximal to the TJ membrane. We also show that the CGN mutant protein that causes deafness in human and mouse models is localized at TJs but does not bind to NM2B, resulting in decreased TJ membrane tortuosity. These results indicate that the interaction between CGN and NM2B regulates epithelial tissue morphogenesis and nanoscale organization of CGN and suggest that CGN regulates the auditory function of hair cells by organizing the actomyosin cytoskeleton to modulate the mechanics of the apical and junctional cortex. Highlighted Article: The interaction between cingulin and nonmuscle myosin regulates cyst formation of MDCK cells and maintains the nanoscale organization of cingulin. The deafness-associated cingulin variant affects tight junction membrane mechanics. Cingulin (CGN) tethers nonmuscle myosin 2B (NM2B; heavy chain encoded by MYH10) to tight junctions (TJs) to modulate junctional and apical cortex mechanics. Here, we studied the role of the CGN-nonmuscle myosin 2 (NM2) interaction in epithelial morphogenesis and nanoscale organization of CGN by expressing wild-type and mutant CGN constructs in CGN-knockout Madin-Darby canine kidney (MDCK) epithelial cells. We show that the NM2-binding region of CGN is required to promote normal cyst morphogenesis of MDCK cells grown in three dimensions and to maintain the C-terminus of CGN in a distal position with respect to the ZO-2 (or TJP2)-containing TJ submembrane region, whereas the N-terminus of CGN is localized more proximal to the TJ membrane. We also show that the CGN mutant protein that causes deafness in human and mouse models is localized at TJs but does not bind to NM2B, resulting in decreased TJ membrane tortuosity. These results indicate that the interaction between CGN and NM2B regulates epithelial tissue morphogenesis and nanoscale organization of CGN and suggest that CGN regulates the auditory function of hair cells by organizing the actomyosin cytoskeleton to modulate the mechanics of the apical and junctional cortex.Cingulin (CGN) tethers nonmuscle myosin 2B (NM2B; heavy chain encoded by MYH10) to tight junctions (TJs) to modulate junctional and apical cortex mechanics. Here, we studied the role of the CGN-nonmuscle myosin 2 (NM2) interaction in epithelial morphogenesis and nanoscale organization of CGN by expressing wild-type and mutant CGN constructs in CGN-knockout Madin-Darby canine kidney (MDCK) epithelial cells. We show that the NM2-binding region of CGN is required to promote normal cyst morphogenesis of MDCK cells grown in three dimensions and to maintain the C-terminus of CGN in a distal position with respect to the ZO-2 (or TJP2)-containing TJ submembrane region, whereas the N-terminus of CGN is localized more proximal to the TJ membrane. We also show that the CGN mutant protein that causes deafness in human and mouse models is localized at TJs but does not bind to NM2B, resulting in decreased TJ membrane tortuosity. These results indicate that the interaction between CGN and NM2B regulates epithelial tissue morphogenesis and nanoscale organization of CGN and suggest that CGN regulates the auditory function of hair cells by organizing the actomyosin cytoskeleton to modulate the mechanics of the apical and junctional cortex. |
Author | Rouaud, Florian Mutero-Maeda, Annick Maupérin, Marine Citi, Sandra |
AuthorAffiliation | Department of Molecular and Cellular Biology , University of Geneva , 30, Quai E. Ansermet, 1205 Geneva , Switzerland |
AuthorAffiliation_xml | – name: Department of Molecular and Cellular Biology , University of Geneva , 30, Quai E. Ansermet, 1205 Geneva , Switzerland |
Author_xml | – sequence: 1 givenname: Florian surname: Rouaud fullname: Rouaud, Florian organization: Department of Molecular and Cellular Biology, University of Geneva, 30, Quai E. Ansermet, 1205 Geneva, Switzerland – sequence: 2 givenname: Marine surname: Maupérin fullname: Maupérin, Marine organization: Department of Molecular and Cellular Biology, University of Geneva, 30, Quai E. Ansermet, 1205 Geneva, Switzerland – sequence: 3 givenname: Annick surname: Mutero-Maeda fullname: Mutero-Maeda, Annick organization: Department of Molecular and Cellular Biology, University of Geneva, 30, Quai E. Ansermet, 1205 Geneva, Switzerland – sequence: 4 givenname: Sandra orcidid: 0000-0002-6537-4818 surname: Citi fullname: Citi, Sandra organization: Department of Molecular and Cellular Biology, University of Geneva, 30, Quai E. Ansermet, 1205 Geneva, Switzerland |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/39319625$$D View this record in MEDLINE/PubMed |
BookMark | eNpVkcFO3DAQhi0EgoX2wgOgHKtKAdvjxPGpqlalrYTEBc6WcSa7Ro6d2kml5ekx2gW1pzn833wz0n9OjkMMSMglo9eMC37zbPM1bzk0cERWTEhZKwbymKwo5axWDcAZOc_5mVIquZKn5AwUMNXyZkXS2oXN4l2oi3RcsvVYjbuYXahcmDEZO7sYqsmbXa5MlWLJS4aTm7fonfHVGNO0jRsMmF1BQl_Zg7IKJsRsTVmJaWOCezFvsk_kZDA-4-fDvCCPtz8e1r_qu_ufv9ff72rL226u-77BoRd84KKRVAnz1MjeKIkDCtsBdtQq4NLKjjUGOsMZArChH7AFKVoJF-Tb3jstTyP2FsOcjNdTcqNJOx2N0_8nwW31Jv7VjAmhhKDF8OVgSPHPgnnWo8sWvTcB45I1sPIXUAltQb_uUZtizgmHjzuM6reWdGlJ71sq8NW_n32g77XAK6VLk2U |
Cites_doi | 10.1016/j.devcel.2005.03.003 10.1016/j.cub.2013.03.039 10.1074/jbc.273.45.29745 10.1242/jcs.261776 10.1016/S1046-2023(03)00032-X 10.1083/jcb.17.2.375 10.1083/jcb.201304194 10.1086/379286 10.3389/fphar.2022.895703 10.1091/mbc.E21-07-0355 10.1016/j.bbamem.2020.183399 10.1101/cshperspect.a028761 10.1242/jcs.01399 10.1101/cshperspect.a029314 10.1083/jcb.147.7.1569 10.1016/j.semcdb.2014.08.011 10.1016/j.jbc.2022.101797 10.1038/s41467-023-43653-w 10.1242/jcs.260745 10.1038/ncomms12426 10.1146/annurev-cellbio-100814-125323 10.1038/s41575-023-00766-3 10.1002/embr.201338128 10.1016/j.cub.2014.06.068 10.1016/j.devcel.2020.07.022 10.1016/j.cell.2019.10.011 10.1038/ncb2106 10.1038/s41598-018-33418-7 10.1083/jcb.202307104 10.1111/j.1749-6632.2012.06506.x 10.15252/emmm.202317611 10.1111/nyas.13391 10.1242/jcs.101261 10.1242/jcs.229286 10.1016/j.cell.2019.10.006 10.1038/s41467-019-09074-4 10.1371/journal.pone.0104994 10.1091/mbc.e06-02-0122 10.1083/jcb.202208065 10.1016/j.cub.2018.02.003 10.1038/333272a0 10.1016/j.cub.2017.11.014 10.1074/jbc.M406563200 10.1007/s12551-019-00582-7 10.7554/eLife.46599 10.1074/jbc.M203717200 |
ContentType | Journal Article |
Copyright | 2024. Published by The Company of Biologists Ltd. 2024. Published by The Company of Biologists Ltd 2024 |
Copyright_xml | – notice: 2024. Published by The Company of Biologists Ltd. – notice: 2024. Published by The Company of Biologists Ltd 2024 |
DBID | CGR CUY CVF ECM EIF NPM AAYXX CITATION 7X8 5PM |
DOI | 10.1242/jcs.262353 |
DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed CrossRef MEDLINE - Academic PubMed Central (Full Participant titles) |
DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) CrossRef MEDLINE - Academic |
DatabaseTitleList | MEDLINE CrossRef MEDLINE - Academic |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Biology |
EISSN | 1477-9137 |
ExternalDocumentID | 10_1242_jcs_262353 39319625 |
Genre | Journal Article |
GrantInformation_xml | – fundername: Canton de Genève – fundername: Université de Genève – fundername: Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung grantid: 31003A_135730 – fundername: ; – fundername: ; grantid: 31003A_135730; 310030_200681; 31003A_172809; 31003A_152899 |
GroupedDBID | --- -DZ -~X 0R~ 18M 34G 39C 4.4 53G 5GY 5RE 85S AAJMC ABDNZ ABJNI ABPPZ ACGFO ACGFS ACIWK ACNCT ACPRK ADBBV AEILP AENEX AFFNX AFRAH AGGIJ ALMA_UNASSIGNED_HOLDINGS BAWUL BTFSW CGR CS3 CUY CVF DIK DU5 E3Z ECM EIF F5P F9R GX1 HZ~ IH2 INIJC KQ8 NPM O9- OK1 P2P R.V RCB RHF RHI RNS SJN TN5 TR2 UPT W2D WH7 WOQ YQT ~02 ~KM AAYXX CITATION 7X8 5PM |
ID | FETCH-LOGICAL-c268t-dd5efd42f2457094ab57da97efe4c83e80c9327c7815a38a21e331fdfe6374673 |
ISSN | 0021-9533 1477-9137 |
IngestDate | Fri Oct 04 05:33:24 EDT 2024 Sat Oct 26 02:06:05 EDT 2024 Fri Dec 06 07:47:57 EST 2024 Sat Nov 02 12:16:40 EDT 2024 |
IsDoiOpenAccess | true |
IsOpenAccess | true |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 18 |
Keywords | Hearing Epithelial cells Cyst morphogenesis Nonmuscle myosin Cingulin |
Language | English |
License | 2024. Published by The Company of Biologists Ltd. http://creativecommons.org/licenses/by/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
LinkModel | OpenURL |
MergedId | FETCHMERGED-LOGICAL-c268t-dd5efd42f2457094ab57da97efe4c83e80c9327c7815a38a21e331fdfe6374673 |
Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Handling Editor: Andrew Ewald The authors declare no competing or financial interests. Competing interests |
ORCID | 0000-0002-6537-4818 |
OpenAccessLink | https://pubmed.ncbi.nlm.nih.gov/PMC11449440 |
PMID | 39319625 |
PQID | 3109430736 |
PQPubID | 23479 |
ParticipantIDs | pubmedcentral_primary_oai_pubmedcentral_nih_gov_11449440 proquest_miscellaneous_3109430736 crossref_primary_10_1242_jcs_262353 pubmed_primary_39319625 |
PublicationCentury | 2000 |
PublicationDate | 2024-Sep-15 |
PublicationDateYYYYMMDD | 2024-09-15 |
PublicationDate_xml | – month: 09 year: 2024 text: 2024-Sep-15 day: 15 |
PublicationDecade | 2020 |
PublicationPlace | England |
PublicationPlace_xml | – name: England |
PublicationTitle | Journal of cell science |
PublicationTitleAlternate | J Cell Sci |
PublicationYear | 2024 |
Publisher | The Company of Biologists Ltd |
Publisher_xml | – name: The Company of Biologists Ltd |
References | Flinois (2024092509532417300_JCS262353C20) 2024; 137 Citi (2024092509532417300_JCS262353C9) 1988; 333 Rouaud (2024092509532417300_JCS262353C31) 2020; 1862 Rouaud (2024092509532417300_JCS262353C32) 2023; 322 Spadaro (2024092509532417300_JCS262353C35) 2017; 27 Guillemot (2024092509532417300_JCS262353C23) 2012; 125 Zhu (2024092509532417300_JCS262353C46) 2023; 15 Buckley (2024092509532417300_JCS262353C6) 2018; 10 Li (2024092509532417300_JCS262353C26) 2014; 15 Nietmann (2024092509532417300_JCS262353C30) 2023; 14 Citi (2024092509532417300_JCS262353C10) 2012; 1257 Fanning (2024092509532417300_JCS262353C18) 1998; 273 Dugina (2024092509532417300_JCS262353C16) 2022; 13 Macara (2024092509532417300_JCS262353C27) 2014; 24 Bryant (2024092509532417300_JCS262353C5) 2010; 12 Du (2024092509532417300_JCS262353C15) 2019; 10 Van Itallie (2024092509532417300_JCS262353C38) 2014; 36 Zhu (2024092509532417300_JCS262353C45) 2003; 73 Vasileva (2024092509532417300_JCS262353C41) 2022; 298 Citi (2024092509532417300_JCS262353C8) 2019; 11 Schwayer (2024092509532417300_JCS262353C33) 2019; 179 Varadarajan (2024092509532417300_JCS262353C39) 2019; 132 Horowitz (2024092509532417300_JCS262353C25) 2023; 20 Belardi (2024092509532417300_JCS262353C2) 2020; 54 Yano (2024092509532417300_JCS262353C43) 2018; 8 Aijaz (2024092509532417300_JCS262353C1) 2005; 8 Beutel (2024092509532417300_JCS262353C3) 2019; 179 Vasileva (2024092509532417300_JCS262353C40) 2017; 1405 Debnath (2024092509532417300_JCS262353C14) 2003; 30 Cordenonsi (2024092509532417300_JCS262353C12) 1999; 147 Blasky (2024092509532417300_JCS262353C4) 2015; 31 Mangan (2024092509532417300_JCS262353C28) 2016; 7 Yano (2024092509532417300_JCS262353C42) 2013; 203 Yap (2024092509532417300_JCS262353C44) 2017; 10 Charras (2024092509532417300_JCS262353C7) 2018; 28 Umeda (2024092509532417300_JCS262353C37) 2004; 279 Guillemot (2024092509532417300_JCS262353C21) 2006; 17 Tokuda (2024092509532417300_JCS262353C36) 2014; 9 Ebrahim (2024092509532417300_JCS262353C17) 2013; 23 Citi (2024092509532417300_JCS262353C11) 2024; 137 D'Atri (2024092509532417300_JCS262353C13) 2002; 277 Farquhar (2024092509532417300_JCS262353C19) 1963; 17 Sluysmans (2024092509532417300_JCS262353C34) 2021; 32 Guillemot (2024092509532417300_JCS262353C22) 2004; 117 Heuze (2024092509532417300_JCS262353C24) 2019; 8 Nguyen (2024092509532417300_JCS262353C29) 2024; 223 |
References_xml | – volume: 8 start-page: 777 year: 2005 ident: 2024092509532417300_JCS262353C1 article-title: Binding of GEF-H1 to the tight junction-associated adaptor cingulin results in inhibition of Rho signaling and G1/S phase transition publication-title: Dev. Cell doi: 10.1016/j.devcel.2005.03.003 contributor: fullname: Aijaz – volume: 23 start-page: 731 year: 2013 ident: 2024092509532417300_JCS262353C17 article-title: NMII forms a contractile transcellular sarcomeric network to regulate apical cell junctions and tissue geometry publication-title: Curr. Biol. doi: 10.1016/j.cub.2013.03.039 contributor: fullname: Ebrahim – volume: 273 start-page: 29745 year: 1998 ident: 2024092509532417300_JCS262353C18 article-title: The tight junction protein ZO-1 establishes a link between the transmembrane protein occludin and the actin cytoskeleton publication-title: J. Biol. Chem. doi: 10.1074/jbc.273.45.29745 contributor: fullname: Fanning – volume: 137 start-page: jcs261776 year: 2024 ident: 2024092509532417300_JCS262353C11 article-title: A short guide to the tight junction publication-title: J. Cell Sci. doi: 10.1242/jcs.261776 contributor: fullname: Citi – volume: 30 start-page: 256 year: 2003 ident: 2024092509532417300_JCS262353C14 article-title: Morphogenesis and oncogenesis of MCF-10A mammary epithelial acini grown in three-dimensional basement membrane cultures publication-title: Methods doi: 10.1016/S1046-2023(03)00032-X contributor: fullname: Debnath – volume: 17 start-page: 375 year: 1963 ident: 2024092509532417300_JCS262353C19 article-title: Junctional complexes in various epithelia publication-title: J. Cell Biol. doi: 10.1083/jcb.17.2.375 contributor: fullname: Farquhar – volume: 203 start-page: 605 year: 2013 ident: 2024092509532417300_JCS262353C42 article-title: The association of microtubules with tight junctions is promoted by cingulin phosphorylation by AMPK publication-title: J. Cell Biol. doi: 10.1083/jcb.201304194 contributor: fullname: Yano – volume: 73 start-page: 1082 year: 2003 ident: 2024092509532417300_JCS262353C45 article-title: Mutations in the gamma-actin gene (ACTG1) are associated with dominant progressive deafness (DFNA20/26) publication-title: Am. J. Hum. Genet. doi: 10.1086/379286 contributor: fullname: Zhu – volume: 13 start-page: 895703 year: 2022 ident: 2024092509532417300_JCS262353C16 article-title: Cytoplasmic beta and gamma actin isoforms reorganization and regulation in tumor cells in culture and tissue publication-title: Front. Pharmacol. doi: 10.3389/fphar.2022.895703 contributor: fullname: Dugina – volume: 32 start-page: 1 year: 2021 ident: 2024092509532417300_JCS262353C34 article-title: PLEKHA5, PLEKHA6 and PLEKHA7 bind to PDZD11 to target the Menkes ATPase ATP7A to the cell periphery and regulate copper homeostasis publication-title: Mol. Biol. Cell doi: 10.1091/mbc.E21-07-0355 contributor: fullname: Sluysmans – volume: 1862 start-page: 183399 year: 2020 ident: 2024092509532417300_JCS262353C31 article-title: Scaffolding proteins of vertebrate apical junctions: structure, functions and biophysics publication-title: Biochim. Biophys. Acta Biomembr. doi: 10.1016/j.bbamem.2020.183399 contributor: fullname: Rouaud – volume: 10 start-page: a028761 year: 2017 ident: 2024092509532417300_JCS262353C44 article-title: Mechanosensing and mechanotransduction at cell-cell junctions publication-title: Cold Spring Harb. Perspect. Biol. doi: 10.1101/cshperspect.a028761 contributor: fullname: Yap – volume: 117 start-page: 5245 year: 2004 ident: 2024092509532417300_JCS262353C22 article-title: Disruption of the cingulin gene does not prevent tight junction formation but alters gene expression publication-title: J. Cell Sci. doi: 10.1242/jcs.01399 contributor: fullname: Guillemot – volume: 10 start-page: a029314 year: 2018 ident: 2024092509532417300_JCS262353C6 article-title: Cell biology of tight junction barrier regulation and mucosal disease publication-title: Cold Spring Harb. Perspect. Biol. doi: 10.1101/cshperspect.a029314 contributor: fullname: Buckley – volume: 147 start-page: 1569 year: 1999 ident: 2024092509532417300_JCS262353C12 article-title: Cingulin contains globular and coiled-coil domains and interacts with ZO-1, ZO-2, ZO-3, and myosin publication-title: J. Cell Biol. doi: 10.1083/jcb.147.7.1569 contributor: fullname: Cordenonsi – volume: 36 start-page: 157 year: 2014 ident: 2024092509532417300_JCS262353C38 article-title: Architecture of tight junctions and principles of molecular composition publication-title: Semin. Cell Dev. Biol. doi: 10.1016/j.semcdb.2014.08.011 contributor: fullname: Van Itallie – volume: 298 start-page: 101797 year: 2022 ident: 2024092509532417300_JCS262353C41 article-title: Cingulin binds to the ZU5 domain of scaffolding protein ZO-1 to promote its extended conformation, stabilization, and tight junction accumulation publication-title: J. Biol. Chem. doi: 10.1016/j.jbc.2022.101797 contributor: fullname: Vasileva – volume: 14 start-page: 7989 year: 2023 ident: 2024092509532417300_JCS262353C30 article-title: Cytosolic actin isoforms form networks with different rheological properties that indicate specific biological function publication-title: Nat. Commun. doi: 10.1038/s41467-023-43653-w contributor: fullname: Nietmann – volume: 137 start-page: jcs260745 year: 2024 ident: 2024092509532417300_JCS262353C20 article-title: Paracingulin recruits CAMSAP3 to tight junctions and regulates microtubule and polarized epithelial cell organization publication-title: J. Cell Sci. doi: 10.1242/jcs.260745 contributor: fullname: Flinois – volume: 7 start-page: 12426 year: 2016 ident: 2024092509532417300_JCS262353C28 article-title: Cingulin and actin mediate midbody-dependent apical lumen formation during polarization of epithelial cells publication-title: Nat. Commun. doi: 10.1038/ncomms12426 contributor: fullname: Mangan – volume: 31 start-page: 575 year: 2015 ident: 2024092509532417300_JCS262353C4 article-title: Polarized protein transport and lumen formation during epithelial tissue morphogenesis publication-title: Annu. Rev. Cell Dev. Biol. doi: 10.1146/annurev-cellbio-100814-125323 contributor: fullname: Blasky – volume: 20 start-page: 417 year: 2023 ident: 2024092509532417300_JCS262353C25 article-title: Paracellular permeability and tight junction regulation in gut health and disease publication-title: Nat. Rev. Gastroenterol. Hepatol. doi: 10.1038/s41575-023-00766-3 contributor: fullname: Horowitz – volume: 15 start-page: 428 year: 2014 ident: 2024092509532417300_JCS262353C26 article-title: FIP5 phosphorylation during mitosis regulates apical trafficking and lumenogenesis publication-title: EMBO Rep. doi: 10.1002/embr.201338128 contributor: fullname: Li – volume: 24 start-page: R815 year: 2014 ident: 2024092509532417300_JCS262353C27 article-title: Epithelial homeostasis publication-title: Curr. Biol. doi: 10.1016/j.cub.2014.06.068 contributor: fullname: Macara – volume: 54 start-page: 792 year: 2020 ident: 2024092509532417300_JCS262353C2 article-title: A weak link with actin organizes tight junctions to control epithelial permeability publication-title: Dev. Cell doi: 10.1016/j.devcel.2020.07.022 contributor: fullname: Belardi – volume: 179 start-page: 923 year: 2019 ident: 2024092509532417300_JCS262353C3 article-title: Phase separation of zonula occludens proteins drives formation of tight junctions publication-title: Cell doi: 10.1016/j.cell.2019.10.011 contributor: fullname: Beutel – volume: 12 start-page: 1035 year: 2010 ident: 2024092509532417300_JCS262353C5 article-title: A molecular network for de novo generation of the apical surface and lumen publication-title: Nat. Cell Biol. doi: 10.1038/ncb2106 contributor: fullname: Bryant – volume: 8 start-page: 15550 year: 2018 ident: 2024092509532417300_JCS262353C43 article-title: AMPK-dependent phosphorylation of cingulin reversibly regulates its binding to actin filaments and microtubules publication-title: Sci. Rep. doi: 10.1038/s41598-018-33418-7 contributor: fullname: Yano – volume: 223 start-page: e202307104 year: 2024 ident: 2024092509532417300_JCS262353C29 article-title: Tight junction membrane proteins regulate the mechanical resistance of the apical junctional complex publication-title: J. Cell Biol. doi: 10.1083/jcb.202307104 contributor: fullname: Nguyen – volume: 1257 start-page: 125 year: 2012 ident: 2024092509532417300_JCS262353C10 article-title: Cingulin, paracingulin, and PLEKHA7: signaling and cytoskeletal adaptors at the apical junctional complex publication-title: Ann. NY Acad. Sci. doi: 10.1111/j.1749-6632.2012.06506.x contributor: fullname: Citi – volume: 15 start-page: e17611 year: 2023 ident: 2024092509532417300_JCS262353C46 article-title: Cingulin regulates hair cell cuticular plate morphology and is required for hearing in human and mouse publication-title: EMBO Mol. Med. doi: 10.15252/emmm.202317611 contributor: fullname: Zhu – volume: 1405 start-page: 160 year: 2017 ident: 2024092509532417300_JCS262353C40 article-title: Cell-specific diversity in the expression and organization of cytoplasmic plaque proteins of apical junctions publication-title: Ann. NY. Acad. Sci. doi: 10.1111/nyas.13391 contributor: fullname: Vasileva – volume: 125 start-page: 5005 year: 2012 ident: 2024092509532417300_JCS262353C23 article-title: Cingulin is dispensable for epithelial barrier function and tight junction structure, and plays a role in the control of claudin-2 expression and response to duodenal mucosa injury publication-title: J. Cell Sci. doi: 10.1242/jcs.101261 contributor: fullname: Guillemot – volume: 132 start-page: jcs229286 year: 2019 ident: 2024092509532417300_JCS262353C39 article-title: Multiscale dynamics of tight junction remodeling publication-title: J. Cell Sci. doi: 10.1242/jcs.229286 contributor: fullname: Varadarajan – volume: 179 start-page: 937 year: 2019 ident: 2024092509532417300_JCS262353C33 article-title: Mechanosensation of tight junctions depends on ZO-1 phase separation and flow publication-title: Cell doi: 10.1016/j.cell.2019.10.006 contributor: fullname: Schwayer – volume: 10 start-page: 1117 year: 2019 ident: 2024092509532417300_JCS262353C15 article-title: LMO7 deficiency reveals the significance of the cuticular plate for hearing function publication-title: Nat. Commun. doi: 10.1038/s41467-019-09074-4 contributor: fullname: Du – volume: 9 start-page: e104994 year: 2014 ident: 2024092509532417300_JCS262353C36 article-title: ZO-1 knockout by TALEN-mediated gene targeting in MDCK cells: involvement of ZO-1 in the regulation of cytoskeleton and cell shape publication-title: PLoS One doi: 10.1371/journal.pone.0104994 contributor: fullname: Tokuda – volume: 17 start-page: 3569 year: 2006 ident: 2024092509532417300_JCS262353C21 article-title: Cingulin regulates claudin-2 expression and cell proliferation through the small GTPase RhoA publication-title: Mol. Biol. Cell doi: 10.1091/mbc.e06-02-0122 contributor: fullname: Guillemot – volume: 322 start-page: e202208065 year: 2023 ident: 2024092509532417300_JCS262353C32 article-title: Cingulin and paracingulin tether myosins-2 to junctions to mechanoregulate the plasma membrane publication-title: J. Cell Biol. doi: 10.1083/jcb.202208065 contributor: fullname: Rouaud – volume: 28 start-page: R445 year: 2018 ident: 2024092509532417300_JCS262353C7 article-title: Tensile forces and mechanotransduction at cell-cell junctions publication-title: Curr. Biol. doi: 10.1016/j.cub.2018.02.003 contributor: fullname: Charras – volume: 333 start-page: 272 year: 1988 ident: 2024092509532417300_JCS262353C9 article-title: Cingulin, a new peripheral component of tight junctions publication-title: Nature doi: 10.1038/333272a0 contributor: fullname: Citi – volume: 27 start-page: 3783 year: 2017 ident: 2024092509532417300_JCS262353C35 article-title: Tension-dependent stretching activates ZO-1 to control the junctional localization of its interactors publication-title: Curr. Biol. doi: 10.1016/j.cub.2017.11.014 contributor: fullname: Spadaro – volume: 279 start-page: 44785 year: 2004 ident: 2024092509532417300_JCS262353C37 article-title: Establishment and characterization of cultured epithelial cells lacking expression of ZO-1 publication-title: J. Biol. Chem. doi: 10.1074/jbc.M406563200 contributor: fullname: Umeda – volume: 11 start-page: 783 year: 2019 ident: 2024092509532417300_JCS262353C8 article-title: The mechanobiology of tight junctions publication-title: Biophys. Rev. doi: 10.1007/s12551-019-00582-7 contributor: fullname: Citi – volume: 8 start-page: e46599 year: 2019 ident: 2024092509532417300_JCS262353C24 article-title: Myosin II isoforms play distinct roles in adherens junction biogenesis publication-title: Elife doi: 10.7554/eLife.46599 contributor: fullname: Heuze – volume: 277 start-page: 27757 year: 2002 ident: 2024092509532417300_JCS262353C13 article-title: Evidence for a functional interaction between cingulin and ZO-1 in cultured cells publication-title: J. Biol. Chem. doi: 10.1074/jbc.M203717200 contributor: fullname: D'Atri |
SSID | ssj0007297 |
Score | 2.5060544 |
Snippet | Cingulin (CGN) tethers nonmuscle myosin 2B (NM2B; heavy chain encoded by MYH10) to tight junctions (TJs) to modulate junctional and apical cortex mechanics.... Cingulin (CGN) tethers nonmuscle myosin 2B (NM2B; heavy chain encoded by MYH10 ) to tight junctions (TJs) to modulate junctional and apical cortex mechanics.... |
SourceID | pubmedcentral proquest crossref pubmed |
SourceType | Open Access Repository Aggregation Database Index Database |
SubjectTerms | Animals Dogs Epithelial Cells - metabolism Epithelium - growth & development Epithelium - metabolism Humans Madin Darby Canine Kidney Cells Mice Morphogenesis Myosin Heavy Chains - genetics Myosin Heavy Chains - metabolism Nonmuscle Myosin Type IIB - genetics Nonmuscle Myosin Type IIB - metabolism Protein Binding Tight Junctions - metabolism |
Title | Cingulin-nonmuscle myosin interaction plays a role in epithelial morphogenesis and cingulin nanoscale organization |
URI | https://www.ncbi.nlm.nih.gov/pubmed/39319625 https://www.proquest.com/docview/3109430736 https://pubmed.ncbi.nlm.nih.gov/PMC11449440 |
Volume | 137 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lj9MwELZgERIXxJuygIzgVqXk4Tx6RKutVrBZhGil3iK_slTaJlXSHLq_fmfipEloDwuXqHUqx535Ys-Mx98Q8kUK8CokxjWEAgfFdZgFVoKyOEb5U9_xdE3HEF8FFwv2fekvu5Sg-nTJVkzk7dFzJf-jVWgDveIp2X_Q7L5TaIDPoF-4gobhei8dn8G6g5nkFvjw66qEu-P1Lgfvv2aBKJoy4JsbvivH3CQSIkPIBs9h3GCofJ2DmPNrnO9WhqxZNl2OM57lJSiwLfx026nw0JbF8P-4WUy7LZyKVzV-Zpjl16Ew5tXG7M8XhsAg5kVvdz_GIhO5FXOtTMw3y1rmftwrWZn8g98w2IL3YxYuwwQLc2qzmWYZbhw7hu5loo-0tXNz87UBYXR00gcrAyd9WU5cMOYM9_CQWfvqZzJbXF4m8_Pl_CF5hKSJWGfhx6-OWR6cjLoQTzuKhsoW-v7a9Tw0Xg48kr8Ta3uWyvwZedqohX4zeHlOHujsBXlsio7uXpLiEDXUoIb2UENr1FBOETVwg3aooQPUUFAEbVFD96ihfdS8IovZ-fzswmoqb1jSDaKtpZSvU8Xc1GV-aE8ZF36o-DTUqWYygvfXlvBGhzKMHJ97EXcd7XlOqlIdeFi_xntNTuA_6LeECmZ7WgShFOBoTFkQBdwOtBJaKI_5So7I51akycYQrCTomILgExB8YgQ_Ip9aaScw_yGqeabzqkyQ2ZbhQhWMyBsj_X0_3hQXGNcfkWigl_0PkFt9eCdb_ak51h2HwWiZ_e4eDz4lTzqQvycn26LSH8BU3YqPNcbuAOtDndQ |
link.rule.ids | 230,314,780,784,885,27924,27925 |
linkProvider | Colorado Alliance of Research Libraries |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Cingulin-nonmuscle+myosin+interaction+plays+a+role+in+epithelial+morphogenesis+and+cingulin+nanoscale+organization&rft.jtitle=Journal+of+cell+science&rft.au=Rouaud%2C+Florian&rft.au=Maup%C3%A9rin%2C+Marine&rft.au=Mutero-Maeda%2C+Annick&rft.au=Citi%2C+Sandra&rft.date=2024-09-15&rft.issn=1477-9137&rft.eissn=1477-9137&rft.volume=137&rft.issue=18&rft_id=info:doi/10.1242%2Fjcs.262353&rft.externalDBID=NO_FULL_TEXT |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0021-9533&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0021-9533&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0021-9533&client=summon |