Silencing of MGMT with small interference RNA reversed resistance in human BCUN-resistant glioma cell lines

• Published in: Chinese medical journal Vol. 124; no. 17; pp. 2605 - 2610
• Main Authors: Xie, Si-ming, Fang, Mao, Guo, Hui, Zhong, Xue-yun
• Format: Journal Article
• Language: English
• Published: China Department of Pathology Medical College,J inan University,Guangzhou,Guangdong 510632,China%Department of Traditional Chinese Medicine Medical College,J inan University,Guangzhou,Guangdong 510632,China 01.09.2011
• Subjects: Blotting, Western; Carmustine - pharmacology; Cell Line, Tumor; Cell Proliferation - drug effects; DNA甲基转移酶; Drug Resistance, Neoplasm - drug effects; Drug Resistance, Neoplasm - genetics; Glioma - genetics; Glioma - metabolism; Humans; MGMT; O-Methylguanine-DNA Methyltransferase - genetics; O-Methylguanine-DNA Methyltransferase - metabolism; Reverse Transcriptase Polymerase Chain Reaction; RNA, Small Interfering; Sincalide - metabolism; Western印迹; 小分子干扰RNA; 性逆转; 细胞系; 脑胶质瘤; 阻力; glioma; small interferencing RNA; 1,3-bis(2-chloroethyl)-l-nitrosourea; drug resistance; MGMT

Background Our previous study had cloned two glioma cell lines SWOZ1 and SWOZ2 isolated from parental glioma cell line SWO38. The 1,3-bis（2-chloroethyl）-l-nitrosourea （BCNU） resistance of SWOZ1 was higher than that of SWOZ2. Since Oe-methylguanine-DNA methyltransferase （MGMT） was thought to be close...