A combined protocol for isolation, culture, and patch-clamp recording of dorsal root ganglion neurons

The dorsal root ganglion (DRG) neurons are crucial in transmitting sensory information from the peripheral nervous system to the central nervous system, including touch, pain, temperature, and proprioception. Understanding the functions and mechanisms of DRG neurons is essential for studying sensory...

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Published inBiophysics reports Vol. 11; no. 2; pp. 87 - 95
Main Authors Wang, Ruolin, Lu, Yu, Zhao, Jianbo, Duan, Xueting, Chen, Yang, Zhang, Zhuoyu, Huang, Rong
Format Journal Article
LanguageEnglish
Published China Biophysics Reports Editorial Office 30.04.2025
Subjects
Protocol
Isolation
Sensory dorsal root ganglion neurons
Patch-clamp recording
Culture
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Abstract The dorsal root ganglion (DRG) neurons are crucial in transmitting sensory information from the peripheral nervous system to the central nervous system, including touch, pain, temperature, and proprioception. Understanding the functions and mechanisms of DRG neurons is essential for studying sensory processing and developing efficient treatments for sensory disorders. In addition, electrophysiological patch-clamp recording is a powerful and classical tool to study the functions and mechanisms of the nervous system. Building upon the strategies outlined in published works and our group's abundant research experience in DRG neurons' functions by patch-clamp, we have summarized and put forward a comprehensive step-by-step protocol combining juvenile rat DRG neuron isolation and culture, and patch-clamp recording. This protocol would be a powerful guidance document for neuroscience researchers to study sensory DRG neurons' physiological and pathological functions using electrophysiological tools.
AbstractList The dorsal root ganglion (DRG) neurons are crucial in transmitting sensory information from the peripheral nervous system to the central nervous system, including touch, pain, temperature, and proprioception. Understanding the functions and mechanisms of DRG neurons is essential for studying sensory processing and developing efficient treatments for sensory disorders. In addition, electrophysiological patch-clamp recording is a powerful and classical tool to study the functions and mechanisms of the nervous system. Building upon the strategies outlined in published works and our group's abundant research experience in DRG neurons' functions by patch-clamp, we have summarized and put forward a comprehensive step-by-step protocol combining juvenile rat DRG neuron isolation and culture, and patch-clamp recording. This protocol would be a powerful guidance document for neuroscience researchers to study sensory DRG neurons' physiological and pathological functions using electrophysiological tools.The dorsal root ganglion (DRG) neurons are crucial in transmitting sensory information from the peripheral nervous system to the central nervous system, including touch, pain, temperature, and proprioception. Understanding the functions and mechanisms of DRG neurons is essential for studying sensory processing and developing efficient treatments for sensory disorders. In addition, electrophysiological patch-clamp recording is a powerful and classical tool to study the functions and mechanisms of the nervous system. Building upon the strategies outlined in published works and our group's abundant research experience in DRG neurons' functions by patch-clamp, we have summarized and put forward a comprehensive step-by-step protocol combining juvenile rat DRG neuron isolation and culture, and patch-clamp recording. This protocol would be a powerful guidance document for neuroscience researchers to study sensory DRG neurons' physiological and pathological functions using electrophysiological tools.
The dorsal root ganglion (DRG) neurons are crucial in transmitting sensory information from the peripheral nervous system to the central nervous system, including touch, pain, temperature, and proprioception. Understanding the functions and mechanisms of DRG neurons is essential for studying sensory processing and developing efficient treatments for sensory disorders. In addition, electrophysiological patch-clamp recording is a powerful and classical tool to study the functions and mechanisms of the nervous system. Building upon the strategies outlined in published works and our group's abundant research experience in DRG neurons' functions by patch-clamp, we have summarized and put forward a comprehensive step-by-step protocol combining juvenile rat DRG neuron isolation and culture, and patch-clamp recording. This protocol would be a powerful guidance document for neuroscience researchers to study sensory DRG neurons' physiological and pathological functions using electrophysiological tools.
Author Huang, Rong
Wang, Ruolin
Chen, Yang
Zhao, Jianbo
Duan, Xueting
Lu, Yu
Zhang, Zhuoyu
AuthorAffiliation 1 Neuroscience Research Center, Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi’an Jiaotong University, Xi’an 710000, China
2 Neurological Department of Tongji Hospital, School of Medicine, Tongji University, Shanghai 200333, China
AuthorAffiliation_xml – name: 1 Neuroscience Research Center, Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi’an Jiaotong University, Xi’an 710000, China
– name: 2 Neurological Department of Tongji Hospital, School of Medicine, Tongji University, Shanghai 200333, China
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Issue 2
Keywords Isolation
Sensory dorsal root ganglion neurons
Patch-clamp recording
Culture
Language English
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Ruolin Wang and Yu Lu contributed equally to this work
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Title A combined protocol for isolation, culture, and patch-clamp recording of dorsal root ganglion neurons
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