A novel thermostable α-galactosidase from the thermophilic fungus Thermomyces lanuginosus CBS 395.62/b: Purification and characterization

• Published in: Biochimica et biophysica acta. General subjects Vol. 1770; no. 1; pp. 55 - 62
• Main Authors: Rezessy-Szabó, Judit M., Nguyen, Quang D., Hoschke, Ágoston, Braet, Christophe, Hajós, Gyöngyi, Claeyssens, Marc
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 2007
• Subjects: Purification; Thermomyces lanuginosus; Thermophilic fungus; α-Galactosidase; Purification; α-Galactosidase; Thermophilic fungus; Thermomyces lanuginosus

High levels of an extracellular α-galactosidase are produced by the thermophilic fungus Thermomyces lanuginosus CBS 395.62/b when grown in submerse culture and induced by sucrose. The enzyme was purified 114-fold from the culture supernatant by (NH 4) 2SO 4 fractionation, and by chromatographical st...