Simultaneous determination of 25(OH)D2, 25(OH)D3 and 1α,25(OH)2D3 in human serum by derivatization-liquid chromatography-tandem mass spectrometry

• Published in: Analytical biochemistry Vol. 701; p. 115821
• Main Authors: Wang, Zi-qing, Hao, Li-ping, Meng, Zi-xuan, Zhang, Hao-ran, Kang, Wei-jun, Ai, Lian-feng
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.06.2025
• Subjects: 1α,25-dihydroxyvitamin D3; 25-hydroxyergocalciferol; 25-Hydroxyvitamin D; 25-Hydroxyvitamin D 2 - blood; acetonitrile; Adult; adults; blood serum; Calcifediol - blood; centrifugation; Chromatography, Liquid - methods; Derivatization; Female; Humans; Limit of Detection; Liquid chromatography-tandem mass spectrometry; Male; metabolites; regression analysis; tandem mass spectrometry; Tandem Mass Spectrometry - methods; 1α,25-dihydroxyvitamin D3; Liquid chromatography-tandem mass spectrometry; Derivatization; 25-Hydroxyvitamin D

An LC-MS/MS method was developed and validated for simultaneously quantifying 25-hydroxyvitamin D2, D3, and 1α,25-dihydroxyvitamin D3 in human serum. Protein in 200 μL serum was precipitated with acetonitrile. After centrifugation, the metabolites were derivatized using (diacetoxyiodo)benzene (PIA)...