Simultaneous determination of 25(OH)D2, 25(OH)D3 and 1α,25(OH)2D3 in human serum by derivatization-liquid chromatography-tandem mass spectrometry - Wang, Zi-qing; Hao, Li-ping; Meng, Zi-xuan; Zhang, Hao-ran; Kang, Wei-jun; Ai, Lian-feng (Analytical biochemistry, 2025)
Keywords: 1α,25-dihydroxyvitamin D3; 25-hydroxyergocalciferol; 25-Hydroxyvitamin D; 25-Hydroxyvitamin D 2 - blood; acetonitrile; Adult; adults; blood serum; Calcifediol - blood; centrifugation; Chromatography, Liquid - methods; Derivatization; Female; Humans; Limit of Detection; Liquid chromatography-tandem mass spectrometry; Male; metabolites; regression analysis; tandem mass spectrometry; Tandem Mass Spectrometry - methods; 1α,25-dihydroxyvitamin D3; Liquid chromatography-tandem mass spectrometry; Derivatization; 25-Hydroxyvitamin D