Generation of Synechocystis sp. PCC 6803 mutant with enhanced laccase-like activity

• Published in: Bioresource technology reports Vol. 20; p. 101266
• Main Authors: Hurtado-McCormick, Valentina, Commault, Audrey, Herdean, Andrei, Price, Shawn, Pernice, Mathieu, Ralph, Peter
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.12.2022
• Subjects: ABTS; anthraquinones; decolorization; Dye decolourisation; dyes; ethyl methanesulfonate; Laccase; microbiome; Mutagenesis; mutants; Synechocystis; Dye decolourisation; Synechocystis; Mutagenesis; ABTS; Laccase

Cyanobacteria offer a good alternative to fungi for laccase production at industrial scales. Random mutagenesis approaches with ethyl methanesulfonate were used in combination with enzymatic assays screenings to select a mutant of Synechocystis sp. PCC 6803, A2, with enhanced extracellular laccase-l...