Rapid Analysis of Polar Components in Ophiocordyceps sinensis by Conventional Liquid Chromatography System
Objective To develop a rapid high-performance liquid chromatography coupled with diode array detection （HPLC-DAD） method for the simultaneous determination of six polar compounds in Ophiocordyceps sinensis. Methods A poroshell SB Aq column （50 mm × 4.6 mm, 2.7 μm） and gradient elution were used; The...
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| Published in | Chinese herbal medicines Vol. 6; no. 3; pp. 217 - 221 |
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| Main Authors | , , , , |
| Format | Journal Article |
| Language | English |
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Elsevier B.V
01.08.2014
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| Abstract | Objective To develop a rapid high-performance liquid chromatography coupled with diode array detection （HPLC-DAD） method for the simultaneous determination of six polar compounds in Ophiocordyceps sinensis. Methods A poroshell SB Aq column （50 mm × 4.6 mm, 2.7 μm） and gradient elution were used; The detection wavelength of compounds was set at 260 nm. The chromatographic peaks of the six investigated compounds in sample were identified by comparing their retention times with reference compounds. Results All calibration curves showed good linearity （r〉 0.999） within the tested ranges. The intra- and inter-day precisions of the six analytes were less than 0.8% and 2.1%, respectively, and the recoveries of the six analytes were between 95% and 103%. The validated method was successfully applied to the determination of six polar compounds in O. sinensis samples. Conclusion The poroshell SB Aq column is suitable for the rapid analysis of polar components in Chinese materia medica on conventional HPLC system and the developed HPLC method is also helpful to the quality control of O. sinensis. |
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| AbstractList | To develop a rapid high-performance liquid chromatography coupled with diode array detection (HPLC-DAD) method for the simultaneous determination of six polar compounds in Ophiocordyceps sinensis.
A poroshell SB Aq column (50 mm × 4.6 mm, 2.7 μm) and gradient elution were used; The detection wavelength of compounds was set at 260 nm. The chromatographic peaks of the six investigated compounds in sample were identified by comparing their retention times with reference compounds.
All calibration curves showed good linearity (r > 0.999) within the tested ranges. The intra- and inter-day precisions of the six analytes were less than 0.8% and 2.1%, respectively, and the recoveries of the six analytes were between 95% and 103%. The validated method was successfully applied to the determination of six polar compounds in O. sinensis samples.
The poroshell SB Aq column is suitable for the rapid analysis of polar components in Chinese materia medica on conventional HPLC system and the developed HPLC method is also helpful to the quality control of O. sinensis. Objective To develop a rapid high-performance liquid chromatography coupled with diode array detection （HPLC-DAD） method for the simultaneous determination of six polar compounds in Ophiocordyceps sinensis. Methods A poroshell SB Aq column （50 mm × 4.6 mm, 2.7 μm） and gradient elution were used; The detection wavelength of compounds was set at 260 nm. The chromatographic peaks of the six investigated compounds in sample were identified by comparing their retention times with reference compounds. Results All calibration curves showed good linearity （r〉 0.999） within the tested ranges. The intra- and inter-day precisions of the six analytes were less than 0.8% and 2.1%, respectively, and the recoveries of the six analytes were between 95% and 103%. The validated method was successfully applied to the determination of six polar compounds in O. sinensis samples. Conclusion The poroshell SB Aq column is suitable for the rapid analysis of polar components in Chinese materia medica on conventional HPLC system and the developed HPLC method is also helpful to the quality control of O. sinensis. |
| Author | Wen-qing Li Wen-jia Li Zheng-ming Qian Shi-lin Chen Li Xiang |
| AuthorAffiliation | HEC R＆D Center, Dongguan 523859, China Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China |
| Author_xml | – sequence: 1 givenname: Wen-qing surname: Li fullname: Li, Wen-qing organization: HEC R&D Center, Dongguan 523859, China – sequence: 2 givenname: Wen-jia surname: Li fullname: Li, Wen-jia organization: HEC R&D Center, Dongguan 523859, China – sequence: 3 givenname: Zheng-ming surname: Qian fullname: Qian, Zheng-ming email: qianzhengming@hecpharm.com organization: HEC R&D Center, Dongguan 523859, China – sequence: 4 givenname: Shi-lin surname: Chen fullname: Chen, Shi-lin organization: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China – sequence: 5 givenname: Li surname: Xiang fullname: Xiang, Li organization: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China |
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| Cites_doi | 10.1016/j.jpba.2006.01.046 10.1093/chromsci/46.3.233 10.1002/bmc.980 10.1016/j.chroma.2008.07.026 10.1016/j.jpba.2008.09.038 10.1016/j.jchromb.2012.09.005 10.1016/j.jpba.2011.08.005 10.1016/j.chroma.2010.06.062 10.1002/jssc.201100295 10.1016/j.talanta.2007.03.034 |
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| Keywords | adenosine uridine polar compounds inosine guanosine Ophiocordyceps sinensis high-performance liquid chromatography uracil cordycepin |
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| Notes | 12-1410/R Objective To develop a rapid high-performance liquid chromatography coupled with diode array detection （HPLC-DAD） method for the simultaneous determination of six polar compounds in Ophiocordyceps sinensis. Methods A poroshell SB Aq column （50 mm × 4.6 mm, 2.7 μm） and gradient elution were used; The detection wavelength of compounds was set at 260 nm. The chromatographic peaks of the six investigated compounds in sample were identified by comparing their retention times with reference compounds. Results All calibration curves showed good linearity （r〉 0.999） within the tested ranges. The intra- and inter-day precisions of the six analytes were less than 0.8% and 2.1%, respectively, and the recoveries of the six analytes were between 95% and 103%. The validated method was successfully applied to the determination of six polar compounds in O. sinensis samples. Conclusion The poroshell SB Aq column is suitable for the rapid analysis of polar components in Chinese materia medica on conventional HPLC system and the developed HPLC method is also helpful to the quality control of O. sinensis. adenosine; cordycepin; guanosine; high-performance liquid chromatography; inosine;Ophiocordyceps sinensis; polar compounds; uracil; uridine |
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| Snippet | Objective To develop a rapid high-performance liquid chromatography coupled with diode array detection （HPLC-DAD） method for the simultaneous determination of... To develop a rapid high-performance liquid chromatography coupled with diode array detection (HPLC-DAD) method for the simultaneous determination of six polar... |
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| SubjectTerms | adenosine cordycepin guanosine high-performance liquid chromatography HPLC-DAD inosine Ophiocordyceps sinensis polar compounds uracil uridine 二极管阵列检测器 传统 冬虫夏草 极性化合物 极性组分 液相色谱系统 高效液相色谱 |
| Title | Rapid Analysis of Polar Components in Ophiocordyceps sinensis by Conventional Liquid Chromatography System |
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