Individual identification and paternity testing of bulls using microsatellite
The polymorphism distributions of 14 microsatellite loci were detected using the Bovine Paternity PCR Typing Kit (including 11 X-STR) and 3 selected Y-STR microsatellite DNA markers. The genetic diversity were evaluated, and the feasibility of the application to individual identification and paterni...
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Published in | Yíchuán Vol. 31; no. 3; p. 285 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | Chinese |
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China
01.03.2009
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Abstract | The polymorphism distributions of 14 microsatellite loci were detected using the Bovine Paternity PCR Typing Kit (including 11 X-STR) and 3 selected Y-STR microsatellite DNA markers. The genetic diversity were evaluated, and the feasibility of the application to individual identification and paternity testing were discussed. The results showed that all the 14 microsatellite loci had genetic polymorphisms in bulls, and the polymorphism information content (PIC) in loci MCM158 was the biggest (0.888), while the ETH10 was the lowest (0.482). Power of discrimination (DP) value of the 14 STR loci ranged from 0.715 to 0.968. The Cumulate DP (CDP) was 99.99%, and the Cumulate PE (CPE) also reached 99.99%. These results indicate that the 14 microsatellites can be applied to the individual identification. |
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AbstractList | The polymorphism distributions of 14 microsatellite loci were detected using the Bovine Paternity PCR Typing Kit (including 11 X-STR) and 3 selected Y-STR microsatellite DNA markers. The genetic diversity were evaluated, and the feasibility of the application to individual identification and paternity testing were discussed. The results showed that all the 14 microsatellite loci had genetic polymorphisms in bulls, and the polymorphism information content (PIC) in loci MCM158 was the biggest (0.888), while the ETH10 was the lowest (0.482). Power of discrimination (DP) value of the 14 STR loci ranged from 0.715 to 0.968. The Cumulate DP (CDP) was 99.99%, and the Cumulate PE (CPE) also reached 99.99%. These results indicate that the 14 microsatellites can be applied to the individual identification. |
Author | Zhang, Li-Ping Song, Xiu-Zhu Yu, Fu-Qing Wang, Zhi-Gang Liu, Chou-Sheng Zhang, Gui-Xiang Wang, Jing Wei, Yu-Chun Han, Xu |
Author_xml | – sequence: 1 givenname: Jing surname: Wang fullname: Wang, Jing email: jwang_423@126.com organization: Lanzhou Vocational Technology College, Lanzhou, China. jwang_423@126.com – sequence: 2 givenname: Chou-Sheng surname: Liu fullname: Liu, Chou-Sheng – sequence: 3 givenname: Li-Ping surname: Zhang fullname: Zhang, Li-Ping – sequence: 4 givenname: Zhi-Gang surname: Wang fullname: Wang, Zhi-Gang – sequence: 5 givenname: Fu-Qing surname: Yu fullname: Yu, Fu-Qing – sequence: 6 givenname: Gui-Xiang surname: Zhang fullname: Zhang, Gui-Xiang – sequence: 7 givenname: Xiu-Zhu surname: Song fullname: Song, Xiu-Zhu – sequence: 8 givenname: Xu surname: Han fullname: Han, Xu – sequence: 9 givenname: Yu-Chun surname: Wei fullname: Wei, Yu-Chun |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/19273442$$D View this record in MEDLINE/PubMed |
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SubjectTerms | Animals Cattle Chromosomes, Mammalian - genetics DNA Fingerprinting - methods Genetic Variation - genetics Male Microsatellite Repeats - genetics Polymerase Chain Reaction Polymorphism, Genetic - genetics Y Chromosome - genetics |
Title | Individual identification and paternity testing of bulls using microsatellite |
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