Individual identification and paternity testing of bulls using microsatellite

The polymorphism distributions of 14 microsatellite loci were detected using the Bovine Paternity PCR Typing Kit (including 11 X-STR) and 3 selected Y-STR microsatellite DNA markers. The genetic diversity were evaluated, and the feasibility of the application to individual identification and paterni...

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Bibliographic Details
Published inYíchuán Vol. 31; no. 3; p. 285
Main Authors Wang, Jing, Liu, Chou-Sheng, Zhang, Li-Ping, Wang, Zhi-Gang, Yu, Fu-Qing, Zhang, Gui-Xiang, Song, Xiu-Zhu, Han, Xu, Wei, Yu-Chun
Format Journal Article
LanguageChinese
Published China 01.03.2009
Subjects
Animals
Cattle
Chromosomes, Mammalian - genetics
DNA Fingerprinting - methods
Genetic Variation - genetics
Male
Microsatellite Repeats - genetics
Polymerase Chain Reaction
Polymorphism, Genetic - genetics
Y Chromosome - genetics
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Summary:The polymorphism distributions of 14 microsatellite loci were detected using the Bovine Paternity PCR Typing Kit (including 11 X-STR) and 3 selected Y-STR microsatellite DNA markers. The genetic diversity were evaluated, and the feasibility of the application to individual identification and paternity testing were discussed. The results showed that all the 14 microsatellite loci had genetic polymorphisms in bulls, and the polymorphism information content (PIC) in loci MCM158 was the biggest (0.888), while the ETH10 was the lowest (0.482). Power of discrimination (DP) value of the 14 STR loci ranged from 0.715 to 0.968. The Cumulate DP (CDP) was 99.99%, and the Cumulate PE (CPE) also reached 99.99%. These results indicate that the 14 microsatellites can be applied to the individual identification.
ISSN:0253-9772
DOI:10.3724/SP.J.1005.2009.00285