Reliable calibration and validation of phenomenological and hybrid models of high-cell-density fed-batch cultures subject to metabolic overflow

• Published in: Computers & chemical engineering Vol. 186; p. 108706
• Main Authors: Ibáñez, Francisco, Puentes-Cantor, Hernán, Bárzaga-Martell, Lisbel, Saa, Pedro A., Agosin, Eduardo, Pérez-Correa, José Ricardo
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.07.2024
• Subjects: Dynamic optimization; Fed-batch fermentation; High-density cultures; Hybrid models; Overflow metabolism; Physics-informed neural networks; Overflow metabolism; High-density cultures; Hybrid models; Physics-informed neural networks; Dynamic optimization; Fed-batch fermentation

Fed-batch cultures are the preferred operation mode for industrial bioprocesses requiring high cellular densities. Avoids accumulation of major fermentation by-products due to metabolic overflow, increasing process productivity. Reproducible operation at high cell densities is challenging (>100 g...