An Effective Method for Selecting siRNA Target Sequences in Mammalian Cells

RNA interference is a gene-silencing phenomenon triggered by dsRNA (double-stranded RNA) and has been widely used for studying gene functions. The short interfering RNA (siRNA) responsible for RNA interference, however, varies markedly in its gene-silencing efficacy. Because this efficacy depends on...

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Bibliographic Details
Published inCell cycle (Georgetown, Tex.) Vol. 3; no. 6; pp. 788 - 793
Main Authors Takasaki, Shigeru, Kotani, Shuji, Konagaya, Akihiko
Format Journal Article
LanguageEnglish
Published Taylor & Francis 01.06.2004
Subjects
Binding
Biology
Bioscience
Calcium
Cancer
Cell
Cycle
Landes
Organogenesis
Proteins
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Summary:RNA interference is a gene-silencing phenomenon triggered by dsRNA (double-stranded RNA) and has been widely used for studying gene functions. The short interfering RNA (siRNA) responsible for RNA interference, however, varies markedly in its gene-silencing efficacy. Because this efficacy depends on the selected target sequences, we developed an effective selection method based on the gene degradation measure (priority score) defined by positional features of individual nucleotides. We tested this method experimentally by using it to select new siRNA target sequences in the homo sapiens cyclin B1 gene (CCNB1) and confirmed that it selected highly effective gene-silencing sequences. The proposed method will therefore be useful for selecting new siRNA target sequences in mammalian cells.
ISSN:1538-4101
1551-4005
DOI:10.4161/cc.3.6.892