Characterization of Sporothrix schenckii by random amplification of polymorphic DNA assay

• Published in: Chinese medical journal Vol. 116; no. 2; pp. 239 - 242
• Main Author: 刘晓明 廉翠红 等
• Format: Journal Article
• Language: English
• Published: China Department of Dermatology, The First Affiliated Hospital, Dalian Medical University, Dalian 116011, China%Department of Bioengineering, Dalian University of Technology, Dalian 116012, China 01.02.2003
• Subjects: DNA, Fungal - analysis; Humans; Random Amplified Polymorphic DNA Technique; Sporothrix - genetics; 分枝孢菌; 多态性DNA阵列; 病原真菌; 随机扩增; Sporothrix schenckii; random amplification of polymorphic DNA assay; DNA typing
• ISSN: 0366-6999; 2542-5641

Objectives To investigate the DNA polymorphism of Sporothrix schenckii(S.schenckii)and to find the relationship between DNA patterns and geographic areas and clinical manifestations.Method The total DNA was extracted with hexadecyltrimethyl-ammonium gromide.Random Amplification of Polymorphic DNA(RAPD) assay was used to study DNA typing of 24 strains of S.schenckii collected from different aress and isolated from different clinical types.Results Of seven random primers used,three primers(OPAA1,OPD18 AND OPB07)gave good reactions,the sequences of which were 5'-ACCCGACCTG-3',5'-GAGAGCCAAC-3',5'-GGTGACGCAG-3' respectively.The RAPD patterns of the 24 isolated were not completely identical,showing certain degrees of hereditary variability.Different isolates showed a common conserved dna band with the same primer.Dirrerent clinical types showed different genotypes.Conclusion RAPD analysis is useful in DNA typing of S.schenckii,the DNA band type of which is related to geographic origin and Clinical manifestation.