Clone identification of a cutting cultivar shakain using cleaved amplified polymorphic sequence markers in Cryptomeria japonica

• Published in: Journal of the Japanese Forest Society Vol. 88; no. 3; pp. 169 - 173
• Main Authors: Kusano, R.(Kumamoto-ken. Forestry Research Center (Japan)), Ieiri, R, Matsumorto, A, Moriguchi, Y, Tsumura, Y
• Format: Journal Article
• Language: Japanese
• Published: THE JAPANESE FORESTRY SOCIETY 01.06.2006
• Subjects: BOUTURE; CAPS marker; CLASIFICACION; CLASSIFICATION; CLONE; clone classification; CLONES; CRYPTOMERIA JAPONICA; Cryptomeria japonica D. Don; cutting cultivar; CUTTINGS; ESQUEJES; GENETIC MARKERS; IDENTIFICACION; IDENTIFICATION; LAND VARIETIES; MARCADORES GENETICOS; MARQUEUR GENETIQUE; Shakain; VARIEDADES INDIGENAS; VARIETE INDIGENE
• DOI: 10.4005/jjfs.88.169
• ISSN: 1349-8509

Shakain is one of the cutting cultivars of Criptomeria japonica in Kumamoto Prefecture. We established a clone classification system using the CAPS (Cleaved Amplified Polymorphic Sequences) markers for clarifying the clone constitution of this cultivar and utilizing it exactly as a seed resource of cutting seedlings. As a result of screening 33 markers previously reported, six markers showing the stable PCR amplification, easy to genotype specification and co-dominance type were chosen. A classification result of 16 Shakain individuals by these CAPS markers accorded with a result from 6 microsatellite markers. The possibility of clone classification using this marker system was evaluated and we applied these markers for further analysis. Two hundred and twenty Shakain individuals which had been planted around Kumamoto Prefecture were classified into 27 DNA types, and two major DNA types of these accounted for 78.6%. According to the ratio of DNA types in each afforestation area, it was likely that a cultivar Shakain was derived from the selection in the general seedling afforestation area.